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A Probing Feeding Strategy for E. coli Cultivations

Axelsson, Jan Peter; Åkesson, Mats LU and Hagander, Per LU (1999) Abstract book of 217th ACS National Meeting In Abstract book of 217th ACS National Meeting
Abstract
Accumulation of acetate tends to reduce growth and recombinant protein production in E. coli cultivations. Formation of acetate can be avoided by a proper substrate feeding strategy, but most strategies require considerable process knowledge to work well. A feedback strategy is presented that requires minimal a priori information. The key idea is to exploit a characteristic change in the cell metabolism at the onset of acetate formation. By superimposing short pulses in the substrate feed rate, on-line detection of acetate formation can be made using a standard dissolved oxygen sensor. A simple feedback algorithm is then used to adjust the feed rate to avoid acetate formation while maintaining a high growth rate. The strategy was used in... (More)
Accumulation of acetate tends to reduce growth and recombinant protein production in E. coli cultivations. Formation of acetate can be avoided by a proper substrate feeding strategy, but most strategies require considerable process knowledge to work well. A feedback strategy is presented that requires minimal a priori information. The key idea is to exploit a characteristic change in the cell metabolism at the onset of acetate formation. By superimposing short pulses in the substrate feed rate, on-line detection of acetate formation can be made using a standard dissolved oxygen sensor. A simple feedback algorithm is then used to adjust the feed rate to avoid acetate formation while maintaining a high growth rate. The strategy was used in fed-batch cultivations of recombinant E. coli. Acetate accumulation was avoided and a good production of recombinant protein could be achieved at the first attempt for each genetic construction. (Less)
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Abstract book of 217th ACS National Meeting
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Abstract book of 217th ACS National Meeting
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c85c97eb-a478-4e81-a789-a1838b0f62c5 (old id 8516719)
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2016-01-19 10:29:03
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@misc{c85c97eb-a478-4e81-a789-a1838b0f62c5,
  abstract     = {Accumulation of acetate tends to reduce growth and recombinant protein production in E. coli cultivations. Formation of acetate can be avoided by a proper substrate feeding strategy, but most strategies require considerable process knowledge to work well. A feedback strategy is presented that requires minimal a priori information. The key idea is to exploit a characteristic change in the cell metabolism at the onset of acetate formation. By superimposing short pulses in the substrate feed rate, on-line detection of acetate formation can be made using a standard dissolved oxygen sensor. A simple feedback algorithm is then used to adjust the feed rate to avoid acetate formation while maintaining a high growth rate. The strategy was used in fed-batch cultivations of recombinant E. coli. Acetate accumulation was avoided and a good production of recombinant protein could be achieved at the first attempt for each genetic construction.},
  author       = {Axelsson, Jan Peter and Åkesson, Mats and Hagander, Per},
  language     = {eng},
  series       = {Abstract book of 217th ACS National Meeting},
  title        = {A Probing Feeding Strategy for <em>E. coli</em> Cultivations},
  year         = {1999},
}