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Genetic Variability Overrides the Impact of Parental Cell Type and Determines iPSC Differentiation Potential.

Kyttälä, Aija; Moraghebi, Roksana LU ; Valensisi, Cristina; Kettunen, Johannes; Andrus, Colin; Pasumarthy, Kalyan Kumar; Nakanishi, Mahito; Nishimura, Ken; Ohtaka, Manami and Weltner, Jere, et al. (2016) In Stem Cell Reports 6(2). p.200-212
Abstract
Reports on the retention of somatic cell memory in induced pluripotent stem cells (iPSCs) have complicated the selection of the optimal cell type for the generation of iPSC biobanks. To address this issue we compared transcriptomic, epigenetic, and differentiation propensities of genetically matched human iPSCs derived from fibroblasts and blood, two tissues of the most practical relevance for biobanking. Our results show that iPSC lines derived from the same donor are highly similar to each other. However, genetic variation imparts a donor-specific expression and methylation profile in reprogrammed cells that leads to variable functional capacities of iPSC lines. Our results suggest that integration-free, bona fide iPSC lines from... (More)
Reports on the retention of somatic cell memory in induced pluripotent stem cells (iPSCs) have complicated the selection of the optimal cell type for the generation of iPSC biobanks. To address this issue we compared transcriptomic, epigenetic, and differentiation propensities of genetically matched human iPSCs derived from fibroblasts and blood, two tissues of the most practical relevance for biobanking. Our results show that iPSC lines derived from the same donor are highly similar to each other. However, genetic variation imparts a donor-specific expression and methylation profile in reprogrammed cells that leads to variable functional capacities of iPSC lines. Our results suggest that integration-free, bona fide iPSC lines from fibroblasts and blood can be combined in repositories to form biobanks. Due to the impact of genetic variation on iPSC differentiation, biobanks should contain cells from large numbers of donors. (Less)
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Contribution to journal
publication status
published
subject
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Stem Cell Reports
volume
6
issue
2
pages
200 - 212
publisher
Cell Press
external identifiers
  • PMID:26777058
  • Scopus:84957849512
  • WOS:000369726500005
ISSN
2213-6711
DOI
10.1016/j.stemcr.2015.12.009
language
English
LU publication?
yes
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e003998d-71ad-4d7f-9ec2-70ded216e11b (old id 8577359)
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http://www.ncbi.nlm.nih.gov/pubmed/26777058?dopt=Abstract
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2016-02-03 17:44:45
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2016-11-06 04:24:59
@misc{e003998d-71ad-4d7f-9ec2-70ded216e11b,
  abstract     = {Reports on the retention of somatic cell memory in induced pluripotent stem cells (iPSCs) have complicated the selection of the optimal cell type for the generation of iPSC biobanks. To address this issue we compared transcriptomic, epigenetic, and differentiation propensities of genetically matched human iPSCs derived from fibroblasts and blood, two tissues of the most practical relevance for biobanking. Our results show that iPSC lines derived from the same donor are highly similar to each other. However, genetic variation imparts a donor-specific expression and methylation profile in reprogrammed cells that leads to variable functional capacities of iPSC lines. Our results suggest that integration-free, bona fide iPSC lines from fibroblasts and blood can be combined in repositories to form biobanks. Due to the impact of genetic variation on iPSC differentiation, biobanks should contain cells from large numbers of donors.},
  author       = {Kyttälä, Aija and Moraghebi, Roksana and Valensisi, Cristina and Kettunen, Johannes and Andrus, Colin and Pasumarthy, Kalyan Kumar and Nakanishi, Mahito and Nishimura, Ken and Ohtaka, Manami and Weltner, Jere and Van Handel, Ben and Parkkonen, Olavi and Sinisalo, Juha and Jalanko, Anu and Hawkins, R David and Woods, Niels-Bjarne and Otonkoski, Timo and Trokovic, Ras},
  issn         = {2213-6711},
  language     = {eng},
  month        = {01},
  number       = {2},
  pages        = {200--212},
  publisher    = {ARRAY(0xa0906e0)},
  series       = {Stem Cell Reports},
  title        = {Genetic Variability Overrides the Impact of Parental Cell Type and Determines iPSC Differentiation Potential.},
  url          = {http://dx.doi.org/10.1016/j.stemcr.2015.12.009},
  volume       = {6},
  year         = {2016},
}