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Nanohybrid polymer brushes on silica for bioseparation

Jiang, Lingdong LU ; Bagan Navarro, Hector LU ; Kamra, Tripta LU ; Zhou, Tongchang LU and Ye, Lei LU (2016) In Journal of Materials Chemistry. B 4(19). p.3247-3256
Abstract

Boronic acid based affinity materials are of great importance for effective enrichment of biomolecules containing a cis-diol structure, for example glycoproteins. In this work, we developed a new pH- and temperature-responsive boronate affinity material for effective separation of glycoproteins. A nanohybrid material composed of silica cores and flexible polymer brushes, denoted as Si@poly(NIPAm-co-GMA)@APBA, was prepared via surface-initiated atom transfer radical polymerization (SI-ATRP) in combination with Cu(i)-catalyzed azide-alkyne cycloaddition (CuAAC) click reaction. The size, morphology and composition of the obtained nanohybrid were characterized by dynamic light scattering (DLS), scanning electron microscopy (SEM), Fourier... (More)

Boronic acid based affinity materials are of great importance for effective enrichment of biomolecules containing a cis-diol structure, for example glycoproteins. In this work, we developed a new pH- and temperature-responsive boronate affinity material for effective separation of glycoproteins. A nanohybrid material composed of silica cores and flexible polymer brushes, denoted as Si@poly(NIPAm-co-GMA)@APBA, was prepared via surface-initiated atom transfer radical polymerization (SI-ATRP) in combination with Cu(i)-catalyzed azide-alkyne cycloaddition (CuAAC) click reaction. The size, morphology and composition of the obtained nanohybrid were characterized by dynamic light scattering (DLS), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), elemental analysis and thermogravimetric analysis (TGA). The density of polymer brushes on the surface of silica nanoparticles was determined to be 0.7 molecules per nm2. The maximum binding capacities of the nanohybrid Si@poly(NIPAm-co-GMA)@APBA for ovalbumin (OVA) and horseradish peroxidase (HRP) were determined to be 87.6 mg g-1 and 22.8 mg g-1, respectively. Glycoprotein binding on the nanohybrid could be controlled by varying the pH of the binding buffer. By increasing the temperature from 20 °C to 35 °C, glycoprotein binding onto the nanohybrid was decreased. This new pH- and temperature-responsive nanohybrid will be useful for a number of biotechnological and biomedical applications, for example, for protein separation and drug delivery.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Materials Chemistry. B
volume
4
issue
19
pages
10 pages
publisher
Royal Society of Chemistry
external identifiers
  • Scopus:84969964561
ISSN
2050-7518
DOI
10.1039/c6tb00241b
language
English
LU publication?
yes
id
85d206f3-1231-40bc-9d3d-cd0075240f14
date added to LUP
2016-07-19 07:50:04
date last changed
2016-07-19 07:50:04
@misc{85d206f3-1231-40bc-9d3d-cd0075240f14,
  abstract     = {<p>Boronic acid based affinity materials are of great importance for effective enrichment of biomolecules containing a cis-diol structure, for example glycoproteins. In this work, we developed a new pH- and temperature-responsive boronate affinity material for effective separation of glycoproteins. A nanohybrid material composed of silica cores and flexible polymer brushes, denoted as Si@poly(NIPAm-co-GMA)@APBA, was prepared via surface-initiated atom transfer radical polymerization (SI-ATRP) in combination with Cu(i)-catalyzed azide-alkyne cycloaddition (CuAAC) click reaction. The size, morphology and composition of the obtained nanohybrid were characterized by dynamic light scattering (DLS), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), elemental analysis and thermogravimetric analysis (TGA). The density of polymer brushes on the surface of silica nanoparticles was determined to be 0.7 molecules per nm<sup>2</sup>. The maximum binding capacities of the nanohybrid Si@poly(NIPAm-co-GMA)@APBA for ovalbumin (OVA) and horseradish peroxidase (HRP) were determined to be 87.6 mg g<sup>-1</sup> and 22.8 mg g<sup>-1</sup>, respectively. Glycoprotein binding on the nanohybrid could be controlled by varying the pH of the binding buffer. By increasing the temperature from 20 °C to 35 °C, glycoprotein binding onto the nanohybrid was decreased. This new pH- and temperature-responsive nanohybrid will be useful for a number of biotechnological and biomedical applications, for example, for protein separation and drug delivery.</p>},
  author       = {Jiang, Lingdong and Bagan Navarro, Hector and Kamra, Tripta and Zhou, Tongchang and Ye, Lei},
  issn         = {2050-7518},
  language     = {eng},
  number       = {19},
  pages        = {3247--3256},
  publisher    = {ARRAY(0x9eb8508)},
  series       = {Journal of Materials Chemistry. B},
  title        = {Nanohybrid polymer brushes on silica for bioseparation},
  url          = {http://dx.doi.org/10.1039/c6tb00241b},
  volume       = {4},
  year         = {2016},
}