Advanced

Erratum : Binding of a new Ca2+ sensitizer, levosimendan, to recombinant human cardiac troponin C. A molecular modeling, fluorescence probe, and proton magnetic resonance study (Journal of Biological Chemistry (1994) 269 (28584-28590))

Pollesello, P.; Ovaska, M.; Kaivola, J.; Tilgmann, C. LU ; Lundstrom, K.; Kalkkinen, N.; Ulmanen, I.; Nissinen, E. and Taskinen, J. (1995) In Journal of Biological Chemistry 270(6). p.2880-2880
Abstract
The binding of a new calcium sensitizer, levosimendan, to human cardiac troponin C (cTnC) is described. Fluorescence studies done on dansylated recombinant human cTnC and a site-directed mutant showed that levosimendan modulated the calcium-induced conformational change in cTnC, and revealed the role of Asp-88 in the binding of the drug to the NH2-terminal domain of cTnC. Furthermore, NMR studies performed on the NH2-terminal fragment of cTnC showed a spatial proximity between levosimendan and Met81, Met85, and Phe77 in the drug-protein complex. These data were used to build an optimized model of the drug-protein complex, in which levosimendan binds cTnC at the hydrophobic pocket of the NH2-terminal domain. The role of the binding of... (More)
The binding of a new calcium sensitizer, levosimendan, to human cardiac troponin C (cTnC) is described. Fluorescence studies done on dansylated recombinant human cTnC and a site-directed mutant showed that levosimendan modulated the calcium-induced conformational change in cTnC, and revealed the role of Asp-88 in the binding of the drug to the NH2-terminal domain of cTnC. Furthermore, NMR studies performed on the NH2-terminal fragment of cTnC showed a spatial proximity between levosimendan and Met81, Met85, and Phe77 in the drug-protein complex. These data were used to build an optimized model of the drug-protein complex, in which levosimendan binds cTnC at the hydrophobic pocket of the NH2-terminal domain. The role of the binding of levosimendan to cTnC in the pharmacological action of this drug in vivo is discussed. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Calcium/metabolism*, Troponin C, simendan, Myocardium/metabolism*
in
Journal of Biological Chemistry
volume
270
issue
6
pages
1 pages
publisher
American Society for Biochemistry and Molecular Biology
external identifiers
  • Scopus:0028893722
ISSN
0021-9258
DOI
10.1074/jbc.270.6.2880
language
English
LU publication?
yes
id
8739a2d5-61c2-453b-a656-1194898e08c1
date added to LUP
2016-04-11 13:21:22
date last changed
2016-08-30 12:38:52
@misc{8739a2d5-61c2-453b-a656-1194898e08c1,
  abstract     = {The binding of a new calcium sensitizer, levosimendan, to human cardiac troponin C (cTnC) is described. Fluorescence studies done on dansylated recombinant human cTnC and a site-directed mutant showed that levosimendan modulated the calcium-induced conformational change in cTnC, and revealed the role of Asp-88 in the binding of the drug to the NH2-terminal domain of cTnC. Furthermore, NMR studies performed on the NH2-terminal fragment of cTnC showed a spatial proximity between levosimendan and Met81, Met85, and Phe77 in the drug-protein complex. These data were used to build an optimized model of the drug-protein complex, in which levosimendan binds cTnC at the hydrophobic pocket of the NH2-terminal domain. The role of the binding of levosimendan to cTnC in the pharmacological action of this drug in vivo is discussed.},
  author       = {Pollesello, P. and Ovaska, M. and Kaivola, J. and Tilgmann, C. and Lundstrom, K. and Kalkkinen, N. and Ulmanen, I. and Nissinen, E. and Taskinen, J.},
  issn         = {0021-9258},
  keyword      = {Calcium/metabolism*,Troponin C,simendan,Myocardium/metabolism*},
  language     = {eng},
  number       = {6},
  pages        = {2880--2880},
  publisher    = {ARRAY(0xb3b8748)},
  series       = {Journal of Biological Chemistry},
  title        = {Erratum : Binding of a new Ca2+ sensitizer, levosimendan, to recombinant human cardiac troponin C. A molecular modeling, fluorescence probe, and proton magnetic resonance study (Journal of Biological Chemistry (1994) 269 (28584-28590))},
  url          = {http://dx.doi.org/10.1074/jbc.270.6.2880},
  volume       = {270},
  year         = {1995},
}