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Localization of Tamoxifen in Human Breast Tumor by MALDI Mass Spectrometry Imaging

Végvári, Ákos LU ; Shavkunov, Alexander S; Fehniger, Thomas LU ; Grabau, Dorthe LU ; Niméus, Emma LU and Marko-Varga, György LU (2016) In Clinical and Translational Medicine 5(1).
Abstract
Background: Tamoxifen is used in endocrine treatment of breast cancer to inhibit estrogen signaling. A set of strati‐ ed ER‐positive and ER‐negative tumor sections was subjected to manual deposition of tamoxifen solution in order to investigate its spatial distribution upon exposure to interaction within thin tissue sections.

Methods: The localization of tamoxifen in tumor sections was assessed by matrix assisted laser deposition/ioniza‐ tion mass spectrometry imaging. The images of extracted ion maps were analyzed for comparison of signal intensity distributions.

Results: The precursor ion of tamoxifen (m/z 372.233) displayed heterogeneous signal intensity distributions in his‐ tological compartments of tumor tissue... (More)
Background: Tamoxifen is used in endocrine treatment of breast cancer to inhibit estrogen signaling. A set of strati‐ ed ER‐positive and ER‐negative tumor sections was subjected to manual deposition of tamoxifen solution in order to investigate its spatial distribution upon exposure to interaction within thin tissue sections.

Methods: The localization of tamoxifen in tumor sections was assessed by matrix assisted laser deposition/ioniza‐ tion mass spectrometry imaging. The images of extracted ion maps were analyzed for comparison of signal intensity distributions.

Results: The precursor ion of tamoxifen (m/z 372.233) displayed heterogeneous signal intensity distributions in his‐ tological compartments of tumor tissue sections. The levels of tamoxifen in tumor cells compared with stroma were higher in ER‐positive tissues, whereas ER‐negative tissue sections showed lower signal intensities in tumor cells.

Conclusions: The experimental model was successfully applied on frozen tumor samples allowing for di erentiation between ER groups based on distribution of tamoxifen. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Human breast cancer, Tamoxifen treatment, MALDI‐mass spectrometry imaging, Estrogen receptor strati cation, Drug localization, Drug quanti cation
in
Clinical and Translational Medicine
volume
5
issue
1
publisher
Springer
external identifiers
  • PMID:26965929
ISSN
2001-1326
DOI
10.1186/s40169-016-0090-9
language
English
LU publication?
yes
id
ce36ea2a-ef55-4590-9549-a564db881831 (old id 8837479)
date added to LUP
2016-03-15 14:36:15
date last changed
2016-11-18 12:10:45
@misc{ce36ea2a-ef55-4590-9549-a564db881831,
  abstract     = {Background: Tamoxifen is used in endocrine treatment of breast cancer to inhibit estrogen signaling. A set of strati‐ ed ER‐positive and ER‐negative tumor sections was subjected to manual deposition of tamoxifen solution in order to investigate its spatial distribution upon exposure to interaction within thin tissue sections.<br/><br>
Methods: The localization of tamoxifen in tumor sections was assessed by matrix assisted laser deposition/ioniza‐ tion mass spectrometry imaging. The images of extracted ion maps were analyzed for comparison of signal intensity distributions.<br/><br>
Results: The precursor ion of tamoxifen (m/z 372.233) displayed heterogeneous signal intensity distributions in his‐ tological compartments of tumor tissue sections. The levels of tamoxifen in tumor cells compared with stroma were higher in ER‐positive tissues, whereas ER‐negative tissue sections showed lower signal intensities in tumor cells.<br/><br>
Conclusions: The experimental model was successfully applied on frozen tumor samples allowing for di erentiation between ER groups based on distribution of tamoxifen.},
  author       = {Végvári, Ákos and Shavkunov, Alexander S and Fehniger, Thomas and Grabau, Dorthe and Niméus, Emma and Marko-Varga, György},
  issn         = {2001-1326},
  keyword      = {Human breast cancer,Tamoxifen treatment,MALDI‐mass spectrometry imaging,Estrogen receptor strati cation,Drug localization,Drug quanti cation},
  language     = {eng},
  number       = {1},
  publisher    = {ARRAY(0xc2b1998)},
  series       = {Clinical and Translational Medicine},
  title        = {Localization of Tamoxifen in Human Breast Tumor by MALDI Mass Spectrometry Imaging},
  url          = {http://dx.doi.org/10.1186/s40169-016-0090-9},
  volume       = {5},
  year         = {2016},
}