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Serotonin (5-HT) and 5-HT2A receptor agonists suppress lipolysis in primary rat adipose cells

Hansson, Björn LU ; Benavente, Anya Medina LU ; Fryklund, Claes; Fex, Malin LU and Stenkula, Karin G. LU (2016) In Biochemical and Biophysical Research Communications 474(2). p.357-363
Abstract

Serotonin (5-HT) is a biogenic monoamine that functions both as a neurotransmitter and a circulating hormone. Recently, the metabolic effects of 5-HT have gained interest and peripheral 5-HT has been proposed to influence lipid metabolism in various ways. Here, we investigated the metabolic effects of 5-HT in isolated, primary rat adipose cells. Incubation with 5-HT suppressed β-adrenergically stimulated glycerol release and decreased phosphorylation of protein kinase A (PKA)-dependent substrates, hormone sensitive lipase (Ser563) and perilipin (Ser522). The inhibitory effect of 5-HT on lipolysis enhanced the anti-lipolytic effect of insulin, but sustained in the presence of phosphodiesterase inhibitors, OPC3911 and... (More)

Serotonin (5-HT) is a biogenic monoamine that functions both as a neurotransmitter and a circulating hormone. Recently, the metabolic effects of 5-HT have gained interest and peripheral 5-HT has been proposed to influence lipid metabolism in various ways. Here, we investigated the metabolic effects of 5-HT in isolated, primary rat adipose cells. Incubation with 5-HT suppressed β-adrenergically stimulated glycerol release and decreased phosphorylation of protein kinase A (PKA)-dependent substrates, hormone sensitive lipase (Ser563) and perilipin (Ser522). The inhibitory effect of 5-HT on lipolysis enhanced the anti-lipolytic effect of insulin, but sustained in the presence of phosphodiesterase inhibitors, OPC3911 and isobuthylmethylxanthine (IBMX). The relative expression of 5-HT1A, -2B and -4 receptor class family were significantly higher in adipose tissue compared to adipose cells, whereas 5-HT1D, -2A and -7 were highly expressed in isolated adipose cells. Similar to 5-HT, 5-HT2 receptor agonists reduced lipolysis while 5-HT1 receptor agonists rather decreased non-stimulated and insulin-stimulated glucose uptake. Together, these data provide evidence of a direct effect of 5-HT on adipose cells, where 5-HT suppresses lipolysis and glucose uptake, which could contribute to altered systemic lipid- and glucose metabolism.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
5-HT, Adipose cells, Lipid metabolism, Lipolysis, Serotonin
in
Biochemical and Biophysical Research Communications
volume
474
issue
2
pages
7 pages
publisher
Elsevier
external identifiers
  • Scopus:84964607614
ISSN
0006-291X
DOI
10.1016/j.bbrc.2016.04.110
language
English
LU publication?
yes
id
8c193577-5382-451f-b749-d1d4dfc33e1f
date added to LUP
2016-07-04 12:03:30
date last changed
2016-09-27 14:14:26
@misc{8c193577-5382-451f-b749-d1d4dfc33e1f,
  abstract     = {<p>Serotonin (5-HT) is a biogenic monoamine that functions both as a neurotransmitter and a circulating hormone. Recently, the metabolic effects of 5-HT have gained interest and peripheral 5-HT has been proposed to influence lipid metabolism in various ways. Here, we investigated the metabolic effects of 5-HT in isolated, primary rat adipose cells. Incubation with 5-HT suppressed β-adrenergically stimulated glycerol release and decreased phosphorylation of protein kinase A (PKA)-dependent substrates, hormone sensitive lipase (Ser563) and perilipin (Ser522). The inhibitory effect of 5-HT on lipolysis enhanced the anti-lipolytic effect of insulin, but sustained in the presence of phosphodiesterase inhibitors, OPC3911 and isobuthylmethylxanthine (IBMX). The relative expression of 5-HT1A, -2B and -4 receptor class family were significantly higher in adipose tissue compared to adipose cells, whereas 5-HT1D, -2A and -7 were highly expressed in isolated adipose cells. Similar to 5-HT, 5-HT2 receptor agonists reduced lipolysis while 5-HT1 receptor agonists rather decreased non-stimulated and insulin-stimulated glucose uptake. Together, these data provide evidence of a direct effect of 5-HT on adipose cells, where 5-HT suppresses lipolysis and glucose uptake, which could contribute to altered systemic lipid- and glucose metabolism.</p>},
  author       = {Hansson, Björn and Benavente, Anya Medina and Fryklund, Claes and Fex, Malin and Stenkula, Karin G.},
  issn         = {0006-291X},
  keyword      = {5-HT,Adipose cells,Lipid metabolism,Lipolysis,Serotonin},
  language     = {eng},
  month        = {05},
  number       = {2},
  pages        = {357--363},
  publisher    = {ARRAY(0xab4f8e8)},
  series       = {Biochemical and Biophysical Research Communications},
  title        = {Serotonin (5-HT) and 5-HT2A receptor agonists suppress lipolysis in primary rat adipose cells},
  url          = {http://dx.doi.org/10.1016/j.bbrc.2016.04.110},
  volume       = {474},
  year         = {2016},
}