Serotonin (5-HT) and 5-HT2A receptor agonists suppress lipolysis in primary rat adipose cells
(2016) In Biochemical and Biophysical Research Communications 474(2). p.357-363- Abstract
Serotonin (5-HT) is a biogenic monoamine that functions both as a neurotransmitter and a circulating hormone. Recently, the metabolic effects of 5-HT have gained interest and peripheral 5-HT has been proposed to influence lipid metabolism in various ways. Here, we investigated the metabolic effects of 5-HT in isolated, primary rat adipose cells. Incubation with 5-HT suppressed β-adrenergically stimulated glycerol release and decreased phosphorylation of protein kinase A (PKA)-dependent substrates, hormone sensitive lipase (Ser563) and perilipin (Ser522). The inhibitory effect of 5-HT on lipolysis enhanced the anti-lipolytic effect of insulin, but sustained in the presence of phosphodiesterase inhibitors, OPC3911 and... (More)
Serotonin (5-HT) is a biogenic monoamine that functions both as a neurotransmitter and a circulating hormone. Recently, the metabolic effects of 5-HT have gained interest and peripheral 5-HT has been proposed to influence lipid metabolism in various ways. Here, we investigated the metabolic effects of 5-HT in isolated, primary rat adipose cells. Incubation with 5-HT suppressed β-adrenergically stimulated glycerol release and decreased phosphorylation of protein kinase A (PKA)-dependent substrates, hormone sensitive lipase (Ser563) and perilipin (Ser522). The inhibitory effect of 5-HT on lipolysis enhanced the anti-lipolytic effect of insulin, but sustained in the presence of phosphodiesterase inhibitors, OPC3911 and isobuthylmethylxanthine (IBMX). The relative expression of 5-HT1A, -2B and -4 receptor class family were significantly higher in adipose tissue compared to adipose cells, whereas 5-HT1D, -2A and -7 were highly expressed in isolated adipose cells. Similar to 5-HT, 5-HT2 receptor agonists reduced lipolysis while 5-HT1 receptor agonists rather decreased non-stimulated and insulin-stimulated glucose uptake. Together, these data provide evidence of a direct effect of 5-HT on adipose cells, where 5-HT suppresses lipolysis and glucose uptake, which could contribute to altered systemic lipid- and glucose metabolism.
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- author
- Hansson, Björn LU ; Benavente, Anya Medina LU ; Fryklund, Claes ; Fex, Malin LU and Stenkula, Karin G. LU
- organization
- publishing date
- 2016-05-27
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- 5-HT, Adipose cells, Lipid metabolism, Lipolysis, Serotonin
- in
- Biochemical and Biophysical Research Communications
- volume
- 474
- issue
- 2
- pages
- 7 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:84964607614
- pmid:27109474
- wos:000376221700021
- ISSN
- 0006-291X
- DOI
- 10.1016/j.bbrc.2016.04.110
- language
- English
- LU publication?
- yes
- id
- 8c193577-5382-451f-b749-d1d4dfc33e1f
- date added to LUP
- 2016-07-04 12:03:30
- date last changed
- 2024-04-05 01:49:21
@article{8c193577-5382-451f-b749-d1d4dfc33e1f,
abstract = {{<p>Serotonin (5-HT) is a biogenic monoamine that functions both as a neurotransmitter and a circulating hormone. Recently, the metabolic effects of 5-HT have gained interest and peripheral 5-HT has been proposed to influence lipid metabolism in various ways. Here, we investigated the metabolic effects of 5-HT in isolated, primary rat adipose cells. Incubation with 5-HT suppressed β-adrenergically stimulated glycerol release and decreased phosphorylation of protein kinase A (PKA)-dependent substrates, hormone sensitive lipase (Ser563) and perilipin (Ser522). The inhibitory effect of 5-HT on lipolysis enhanced the anti-lipolytic effect of insulin, but sustained in the presence of phosphodiesterase inhibitors, OPC3911 and isobuthylmethylxanthine (IBMX). The relative expression of 5-HT1A, -2B and -4 receptor class family were significantly higher in adipose tissue compared to adipose cells, whereas 5-HT1D, -2A and -7 were highly expressed in isolated adipose cells. Similar to 5-HT, 5-HT2 receptor agonists reduced lipolysis while 5-HT1 receptor agonists rather decreased non-stimulated and insulin-stimulated glucose uptake. Together, these data provide evidence of a direct effect of 5-HT on adipose cells, where 5-HT suppresses lipolysis and glucose uptake, which could contribute to altered systemic lipid- and glucose metabolism.</p>}},
author = {{Hansson, Björn and Benavente, Anya Medina and Fryklund, Claes and Fex, Malin and Stenkula, Karin G.}},
issn = {{0006-291X}},
keywords = {{5-HT; Adipose cells; Lipid metabolism; Lipolysis; Serotonin}},
language = {{eng}},
month = {{05}},
number = {{2}},
pages = {{357--363}},
publisher = {{Elsevier}},
series = {{Biochemical and Biophysical Research Communications}},
title = {{Serotonin (5-HT) and 5-HT2A receptor agonists suppress lipolysis in primary rat adipose cells}},
url = {{http://dx.doi.org/10.1016/j.bbrc.2016.04.110}},
doi = {{10.1016/j.bbrc.2016.04.110}},
volume = {{474}},
year = {{2016}},
}