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Ubiquinone-1 induces external deamino-NADH oxidation in potato tuber mitochondria

Møller, Ian M.; Roberts, Thomas H. and Rasmusson, Allan G. LU (1996) In Plant Physiology 112(1). p.75-78
Abstract

The addition of ubiquinone-1 (UQ-1) induced Ca2+-independent oxidation of deamino-NADH and NADH by intact potato (Solanum tuberosum L. cv Bintje) tuber mitochondria. The induced oxidation was coupled to the generation of a membrane potential. Measurements of NAD+-malate dehydrogenase activity indicated that the permeability of the inner mitochondrial membrane to NADH and deamino-NADH was not altered by the addition of UQ-1. We conclude that UQ-1-induced external deamino-NADH oxidation is due to a change in specificity of the external rotenone-insensitive NADH dehydrogenase. The addition of UQ-1 also induced rotenone-insensitive oxidation of deamino-NADH by inside-out submitochondrial particles, but whether this was... (More)

The addition of ubiquinone-1 (UQ-1) induced Ca2+-independent oxidation of deamino-NADH and NADH by intact potato (Solanum tuberosum L. cv Bintje) tuber mitochondria. The induced oxidation was coupled to the generation of a membrane potential. Measurements of NAD+-malate dehydrogenase activity indicated that the permeability of the inner mitochondrial membrane to NADH and deamino-NADH was not altered by the addition of UQ-1. We conclude that UQ-1-induced external deamino-NADH oxidation is due to a change in specificity of the external rotenone-insensitive NADH dehydrogenase. The addition of UQ-1 also induced rotenone-insensitive oxidation of deamino-NADH by inside-out submitochondrial particles, but whether this was due to a change in the specificity of the internal rotenone-insensitive NAD(P)H dehydrogenase or to a bypass in complex I could not be determined.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Plant Physiology
volume
112
issue
1
pages
4 pages
publisher
American Society of Plant Biologists
external identifiers
  • Scopus:0029911102
ISSN
0032-0889
language
English
LU publication?
yes
id
97f7d801-5855-42d4-9c7b-65619637cc08
alternative location
http://www.plantphysiol.org/content/112/1/75.full.pdf+html
date added to LUP
2016-05-31 21:33:23
date last changed
2016-08-10 14:14:37
@misc{97f7d801-5855-42d4-9c7b-65619637cc08,
  abstract     = {<p>The addition of ubiquinone-1 (UQ-1) induced Ca<sup>2+</sup>-independent oxidation of deamino-NADH and NADH by intact potato (Solanum tuberosum L. cv Bintje) tuber mitochondria. The induced oxidation was coupled to the generation of a membrane potential. Measurements of NAD<sup>+</sup>-malate dehydrogenase activity indicated that the permeability of the inner mitochondrial membrane to NADH and deamino-NADH was not altered by the addition of UQ-1. We conclude that UQ-1-induced external deamino-NADH oxidation is due to a change in specificity of the external rotenone-insensitive NADH dehydrogenase. The addition of UQ-1 also induced rotenone-insensitive oxidation of deamino-NADH by inside-out submitochondrial particles, but whether this was due to a change in the specificity of the internal rotenone-insensitive NAD(P)H dehydrogenase or to a bypass in complex I could not be determined.</p>},
  author       = {Møller, Ian M. and Roberts, Thomas H. and Rasmusson, Allan G.},
  issn         = {0032-0889},
  language     = {eng},
  number       = {1},
  pages        = {75--78},
  publisher    = {ARRAY(0xb300960)},
  series       = {Plant Physiology},
  title        = {Ubiquinone-1 induces external deamino-NADH oxidation in potato tuber mitochondria},
  volume       = {112},
  year         = {1996},
}