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Purification of F.VIII:C by antigen-antibody chromatography

Ljung, R. LU and Holmberg, Lars LU (1978) In Thrombosis Research 12(4). p.667-675
Abstract

Purification of F.VIII:C devoid of F.VIII:Ag was achieved by antigen-antibody chromatography. The antibody used neutralized VIIIR:Ag but not VIII:C in liquid phase but extracted both VIII:Ag and VIII:C from plasma when bound to Sepharose. VIII:C was eluted with calcium-containing buffer. When plasma was used as starting material VIII:C was obtained free from VIIIR:Ag but contaminated with some other proteins. When a well-defined pure F.VIII preparation was used as starting material the VIII:C active fractions contained no immunoradiometrically detectable VIIIR:Ag, no VIIIR:RCF and no detectable protein. When stabilized with albumin VIII:C could be frozen and thawed with retained activity and could be activated with thrombin. © 1978.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Thrombosis Research
volume
12
issue
4
pages
9 pages
publisher
Elsevier
external identifiers
  • Scopus:0018150726
ISSN
0049-3848
DOI
10.1016/0049-3848(78)90256-6
language
English
LU publication?
yes
id
98f17493-c0fd-48e8-83e1-0824ada050e2
date added to LUP
2016-10-26 13:52:29
date last changed
2016-11-27 04:43:40
@misc{98f17493-c0fd-48e8-83e1-0824ada050e2,
  abstract     = {<p>Purification of F.VIII:C devoid of F.VIII:Ag was achieved by antigen-antibody chromatography. The antibody used neutralized VIIIR:Ag but not VIII:C in liquid phase but extracted both VIII:Ag and VIII:C from plasma when bound to Sepharose. VIII:C was eluted with calcium-containing buffer. When plasma was used as starting material VIII:C was obtained free from VIIIR:Ag but contaminated with some other proteins. When a well-defined pure F.VIII preparation was used as starting material the VIII:C active fractions contained no immunoradiometrically detectable VIIIR:Ag, no VIIIR:RCF and no detectable protein. When stabilized with albumin VIII:C could be frozen and thawed with retained activity and could be activated with thrombin. © 1978.</p>},
  author       = {Ljung, R. and Holmberg, Lars},
  issn         = {0049-3848},
  language     = {eng},
  number       = {4},
  pages        = {667--675},
  publisher    = {ARRAY(0xa1e80e8)},
  series       = {Thrombosis Research},
  title        = {Purification of F.VIII:C by antigen-antibody chromatography},
  url          = {http://dx.doi.org/10.1016/0049-3848(78)90256-6},
  volume       = {12},
  year         = {1978},
}