Capto™ Resins for Chromatography of DNA : A Minor Difference in Ligand Composition Greatly Influences the Separation of Guanidyl-Containing Fragments
(2016) In Chromatographia 79(19-20). p.1277-1282- Abstract
The differences in chromatographic behaviour of individual deoxynucleotides as well as small single-stranded and double-stranded DNA molecules have been examined for two resins from the Capto family: Capto Adhere and Capto Q ImpRes. Capto Adhere carries a multimodal ligand which combines strong anion with aromatic recognition, while Capto Q ImpRes is a strong anion exchanger with a chemically similar ligand, but without a phenyl group. The intrinsic differences between single- and double-stranded DNAs are related to charge, hydrophobicity, size and three-dimensional structure. These variations in biophysical properties have been utilized for comparative separations on these two resins. All deoxynucleotides and DNAs tested bound strongly... (More)
The differences in chromatographic behaviour of individual deoxynucleotides as well as small single-stranded and double-stranded DNA molecules have been examined for two resins from the Capto family: Capto Adhere and Capto Q ImpRes. Capto Adhere carries a multimodal ligand which combines strong anion with aromatic recognition, while Capto Q ImpRes is a strong anion exchanger with a chemically similar ligand, but without a phenyl group. The intrinsic differences between single- and double-stranded DNAs are related to charge, hydrophobicity, size and three-dimensional structure. These variations in biophysical properties have been utilized for comparative separations on these two resins. All deoxynucleotides and DNAs tested bound strongly to the chromatographic materials and could be eluted by a linear gradient of increasing NaCl concentration. Capto Q ImpRes provided a recognition for guanylate bases when samples of deoxynucleotides or poly(dG) were examined. This recognition was not observed for Capto Adhere. Another pronounced difference between the resins was observed in the inverted elution of ss- and dsDNA, where ssDNA eluted at 2.88 M NaCl on Capto Adhere, while on Capto Q ImpRes ssDNA eluted already at 1.47 M NaCl. This behaviour can be linked to the presence of the more hydrophobic phenyl group in Capto Adhere, leading to stronger retention of ssDNA molecules, which have a more hydrophobic character due to a higher degree of base exposure.
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- author
- Matos, Tiago LU ; Mohamed, Elsayed T. ; Queiroz, João A. and Bülow, Leif LU
- organization
- publishing date
- 2016-10-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Double-stranded DNA, Ion-exchange chromatography, Nucleotide, Oligodeoxynucleotide, Plasmid, Single-stranded DNA
- in
- Chromatographia
- volume
- 79
- issue
- 19-20
- pages
- 6 pages
- publisher
- Vieweg Verlag
- external identifiers
-
- scopus:84980007094
- wos:000387326400006
- ISSN
- 0009-5893
- DOI
- 10.1007/s10337-016-3148-3
- language
- English
- LU publication?
- yes
- id
- 9a20ff31-e682-4655-8710-3532a455686a
- date added to LUP
- 2016-10-17 10:33:32
- date last changed
- 2024-03-07 14:09:22
@article{9a20ff31-e682-4655-8710-3532a455686a,
abstract = {{<p>The differences in chromatographic behaviour of individual deoxynucleotides as well as small single-stranded and double-stranded DNA molecules have been examined for two resins from the Capto family: Capto Adhere and Capto Q ImpRes. Capto Adhere carries a multimodal ligand which combines strong anion with aromatic recognition, while Capto Q ImpRes is a strong anion exchanger with a chemically similar ligand, but without a phenyl group. The intrinsic differences between single- and double-stranded DNAs are related to charge, hydrophobicity, size and three-dimensional structure. These variations in biophysical properties have been utilized for comparative separations on these two resins. All deoxynucleotides and DNAs tested bound strongly to the chromatographic materials and could be eluted by a linear gradient of increasing NaCl concentration. Capto Q ImpRes provided a recognition for guanylate bases when samples of deoxynucleotides or poly(dG) were examined. This recognition was not observed for Capto Adhere. Another pronounced difference between the resins was observed in the inverted elution of ss- and dsDNA, where ssDNA eluted at 2.88 M NaCl on Capto Adhere, while on Capto Q ImpRes ssDNA eluted already at 1.47 M NaCl. This behaviour can be linked to the presence of the more hydrophobic phenyl group in Capto Adhere, leading to stronger retention of ssDNA molecules, which have a more hydrophobic character due to a higher degree of base exposure.</p>}},
author = {{Matos, Tiago and Mohamed, Elsayed T. and Queiroz, João A. and Bülow, Leif}},
issn = {{0009-5893}},
keywords = {{Double-stranded DNA; Ion-exchange chromatography; Nucleotide; Oligodeoxynucleotide; Plasmid; Single-stranded DNA}},
language = {{eng}},
month = {{10}},
number = {{19-20}},
pages = {{1277--1282}},
publisher = {{Vieweg Verlag}},
series = {{Chromatographia}},
title = {{Capto™ Resins for Chromatography of DNA : A Minor Difference in Ligand Composition Greatly Influences the Separation of Guanidyl-Containing Fragments}},
url = {{http://dx.doi.org/10.1007/s10337-016-3148-3}},
doi = {{10.1007/s10337-016-3148-3}},
volume = {{79}},
year = {{2016}},
}