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Purification and partial characterization of rat liver soluble catechol- O-methyltransferase

Tilgmann, Carola LU and Kalkkinen, Nisse (1990) In FEBS Letters 264(1). p.95-99
Abstract

The rat liver soluble catechol-O-methyltransferase (EC 2.1.1.6.) has been purified utilizing a combination of conventional chromatography and HPLC. The purified enzyme has a molecular mass of 25 kDa, a pI of 5.1, and exists in two forms which differ in the nature of their intramolecular disulfide bonds. This difference causes these two protein forms to behave differently in reversed phase chromatography. © 1990.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Catechol-O-methyltransferase, soluble, Methylating enzyme
in
FEBS Letters
volume
264
issue
1
pages
5 pages
publisher
Elsevier
external identifiers
  • Scopus:0025355104
ISSN
0014-5793
DOI
10.1016/0014-5793(90)80774-D
language
English
LU publication?
yes
id
9a9cc68d-8609-4e43-9884-a599a5e42c52
date added to LUP
2016-04-11 13:26:35
date last changed
2016-07-27 09:35:17
@misc{9a9cc68d-8609-4e43-9884-a599a5e42c52,
  abstract     = {<p>The rat liver soluble catechol-O-methyltransferase (EC 2.1.1.6.) has been purified utilizing a combination of conventional chromatography and HPLC. The purified enzyme has a molecular mass of 25 kDa, a pI of 5.1, and exists in two forms which differ in the nature of their intramolecular disulfide bonds. This difference causes these two protein forms to behave differently in reversed phase chromatography. © 1990.</p>},
  author       = {Tilgmann, Carola and Kalkkinen, Nisse},
  issn         = {0014-5793},
  keyword      = {Catechol-O-methyltransferase, soluble,Methylating enzyme},
  language     = {eng},
  month        = {05},
  number       = {1},
  pages        = {95--99},
  publisher    = {ARRAY(0x8932b40)},
  series       = {FEBS Letters},
  title        = {Purification and partial characterization of rat liver soluble catechol- O-methyltransferase},
  url          = {http://dx.doi.org/10.1016/0014-5793(90)80774-D},
  volume       = {264},
  year         = {1990},
}