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Epstein-Barr virus-induced transformation of human B lymphocytes : the effect of l-leucyl-l-leucine methyl ester on inhibitory T cell populations

Ohlin, M. LU ; Kristensson, K.; Carlsson, R. LU and Borrebaeck, C. A K LU (1992) In Immunology Letters 34(3). p.221-228
Abstract

Epstein-Barr virus-mediated transformation of human B lymphocytes is inhibited by human T lymphocytes as well as by interferon-γ. Removal of the inhibitory cell populations is essential in order to achieve successful transformation in vitro. Cells with the capacity to inhibit outgrowth of lymphoblastoid cell lines can be removed by pretreatment of peripheral blood mononuclear cells with l-leucyl-l-leucine methyl ester. This treatment eliminates monocytes, NK-cells and a CD8+ T cell subpopulation. We now show that such treatment also has toxic effects on other human T cell populations. In addition, CD4+ and/or CD8+ lymphocytes are demonstrated to contain effector cell activities which inhibit outgrowth of... (More)

Epstein-Barr virus-mediated transformation of human B lymphocytes is inhibited by human T lymphocytes as well as by interferon-γ. Removal of the inhibitory cell populations is essential in order to achieve successful transformation in vitro. Cells with the capacity to inhibit outgrowth of lymphoblastoid cell lines can be removed by pretreatment of peripheral blood mononuclear cells with l-leucyl-l-leucine methyl ester. This treatment eliminates monocytes, NK-cells and a CD8+ T cell subpopulation. We now show that such treatment also has toxic effects on other human T cell populations. In addition, CD4+ and/or CD8+ lymphocytes are demonstrated to contain effector cell activities which inhibit outgrowth of EBV-transformed B cells. This inhibitory activity is abolished after treatment of peripheral blood mononuclear cells or purified CD4+ T cells with l-leucyl-l-leucine methyl ester. No evidence was found for a selective toxicity against any subset within the CD4+ or CD8+ T cell populations. However, the capacity of the treated cells, both peripheral blood mononuclear cells and purified CD4+ T lymphocytes, to produce mRNA encoding IFN-γ, a protein previously shown to downregulate outgrowth of EBV-transformed B cells, was selectively impaired. The results obtained suggest a role for CD4+ T cells to inhibit EBV-induced transformation of B cells. © 1992.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
B lymphocyte, Epstein-Barr virus, Interferon, l-Leucyl-l-leucine methyl ester, T lymphocyte, Transformation
in
Immunology Letters
volume
34
issue
3
pages
8 pages
publisher
Elsevier
external identifiers
  • Scopus:0026739935
ISSN
0165-2478
DOI
10.1016/0165-2478(92)90217-C
language
English
LU publication?
yes
id
9e4f1de5-f578-4163-865b-9d9336693ad1
date added to LUP
2016-04-20 16:09:07
date last changed
2016-05-16 14:49:18
@misc{9e4f1de5-f578-4163-865b-9d9336693ad1,
  abstract     = {<p>Epstein-Barr virus-mediated transformation of human B lymphocytes is inhibited by human T lymphocytes as well as by interferon-γ. Removal of the inhibitory cell populations is essential in order to achieve successful transformation in vitro. Cells with the capacity to inhibit outgrowth of lymphoblastoid cell lines can be removed by pretreatment of peripheral blood mononuclear cells with l-leucyl-l-leucine methyl ester. This treatment eliminates monocytes, NK-cells and a CD8<sup>+</sup> T cell subpopulation. We now show that such treatment also has toxic effects on other human T cell populations. In addition, CD4<sup>+</sup> and/or CD8<sup>+</sup> lymphocytes are demonstrated to contain effector cell activities which inhibit outgrowth of EBV-transformed B cells. This inhibitory activity is abolished after treatment of peripheral blood mononuclear cells or purified CD4<sup>+</sup> T cells with l-leucyl-l-leucine methyl ester. No evidence was found for a selective toxicity against any subset within the CD4<sup>+</sup> or CD8<sup>+</sup> T cell populations. However, the capacity of the treated cells, both peripheral blood mononuclear cells and purified CD4<sup>+</sup> T lymphocytes, to produce mRNA encoding IFN-γ, a protein previously shown to downregulate outgrowth of EBV-transformed B cells, was selectively impaired. The results obtained suggest a role for CD4<sup>+</sup> T cells to inhibit EBV-induced transformation of B cells. © 1992.</p>},
  author       = {Ohlin, M. and Kristensson, K. and Carlsson, R. and Borrebaeck, C. A K},
  issn         = {0165-2478},
  keyword      = {B lymphocyte,Epstein-Barr virus,Interferon,l-Leucyl-l-leucine methyl ester,T lymphocyte,Transformation},
  language     = {eng},
  number       = {3},
  pages        = {221--228},
  publisher    = {ARRAY(0xba37750)},
  series       = {Immunology Letters},
  title        = {Epstein-Barr virus-induced transformation of human B lymphocytes : the effect of l-leucyl-l-leucine methyl ester on inhibitory T cell populations},
  url          = {http://dx.doi.org/10.1016/0165-2478(92)90217-C},
  volume       = {34},
  year         = {1992},
}