Purification and partial sequence analysis of the soluble catechol-o-methyltransferase from human placenta : Comparison to the rat liver enzyme
Tilgmann, Carola LU
and Kalkkinen, Nisse
(1991)
In Biochemical and Biophysical Research Communications
174(2).
p.995-1002
- Abstract
Catechol-o-methyltransferase from human placenta was purified 1400-fold by hydroxyapatite adsorption, ammonium sulfate precipitation, gel filtration, high performance anion- exchange and reversed-phase chromatography. The purified enzyme has an apparent molecular weight of 26.000, an isoelectric point of 5,3 and is activated ten-fold in the presence of 20mM cysteine. The enzyme shows primary structure homology to the corresponding rat liver soluble enzyme, based on the sequenced tryptic peptides.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/a7f41c5e-3775-467d-bf38-3d2560470f90
- author
- Tilgmann, Carola
LU
and Kalkkinen, Nisse
- publishing date
- 1991-01-31
- type
- Contribution to journal
- publication status
- published
- in
- Biochemical and Biophysical Research Communications
- volume
- 174
- issue
- 2
- pages
- 8 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:0025978802
- pmid:1993083
- ISSN
- 0006-291X
- DOI
- 10.1016/0006-291X(91)91517-G
- language
- English
- LU publication?
- no
- id
- a7f41c5e-3775-467d-bf38-3d2560470f90
- date added to LUP
- 2016-04-11 13:25:49
- date last changed
- 2024-01-04 01:06:12
@article{a7f41c5e-3775-467d-bf38-3d2560470f90,
abstract = {{<p>Catechol-o-methyltransferase from human placenta was purified 1400-fold by hydroxyapatite adsorption, ammonium sulfate precipitation, gel filtration, high performance anion- exchange and reversed-phase chromatography. The purified enzyme has an apparent molecular weight of 26.000, an isoelectric point of 5,3 and is activated ten-fold in the presence of 20mM cysteine. The enzyme shows primary structure homology to the corresponding rat liver soluble enzyme, based on the sequenced tryptic peptides.</p>}},
author = {{Tilgmann, Carola and Kalkkinen, Nisse}},
issn = {{0006-291X}},
language = {{eng}},
month = {{01}},
number = {{2}},
pages = {{995--1002}},
publisher = {{Elsevier}},
series = {{Biochemical and Biophysical Research Communications}},
title = {{Purification and partial sequence analysis of the soluble catechol-o-methyltransferase from human placenta : Comparison to the rat liver enzyme}},
url = {{http://dx.doi.org/10.1016/0006-291X(91)91517-G}},
doi = {{10.1016/0006-291X(91)91517-G}},
volume = {{174}},
year = {{1991}},
}