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Epigenetic and Transcriptional Alterations in Human Adipose Tissue of Polycystic Ovary Syndrome

Kokosar, Milana; Benrick, Anna; Perfilyev, Alexander LU ; Fornes, Romina; Nilsson, Emma A LU ; Maliqueo, Manuel; Behre, Carl Johan; Sazonova, Antonina; Ohlsson, Claes and Ling, Charlotte LU , et al. (2016) In Scientific Reports 6.
Abstract

Genetic and epigenetic factors may predispose women to polycystic ovary syndrome (PCOS), a common heritable disorder of unclear etiology. Here we investigated differences in genome-wide gene expression and DNA methylation in adipose tissue from 64 women with PCOS and 30 controls. In total, 1720 unique genes were differentially expressed (Q < 0.05). Six out of twenty selected genes with largest expression difference (CYP1B1, GPT), genes linked to PCOS (RAB5B) or type 2 diabetes (PPARG, SVEP1), and methylation (DMAP1) were replicated in a separate case-control study. In total, 63,213 sites (P < 0.05) and 440 sites (Q < 0.15) were differently methylated. Thirty differentially expressed genes had corresponding changes in 33... (More)

Genetic and epigenetic factors may predispose women to polycystic ovary syndrome (PCOS), a common heritable disorder of unclear etiology. Here we investigated differences in genome-wide gene expression and DNA methylation in adipose tissue from 64 women with PCOS and 30 controls. In total, 1720 unique genes were differentially expressed (Q < 0.05). Six out of twenty selected genes with largest expression difference (CYP1B1, GPT), genes linked to PCOS (RAB5B) or type 2 diabetes (PPARG, SVEP1), and methylation (DMAP1) were replicated in a separate case-control study. In total, 63,213 sites (P < 0.05) and 440 sites (Q < 0.15) were differently methylated. Thirty differentially expressed genes had corresponding changes in 33 different DNA methylation sites. Moreover, a total number of 1913 pairs of differentially expressed "gene-CpG" probes were significantly correlated after correction for multiple testing and corresponded with 349 unique genes. In conclusion, we identified a large number of genes and pathways that are affected in adipose tissue from women with PCOS. We also identified specific DNA methylation pathways that may affect mRNA expression. Together, these novel findings show that women with PCOS have multiple transcriptional and epigenetic changes in adipose tissue that are relevant for development of the disease.

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Scientific Reports
volume
6
publisher
Nature Publishing Group
external identifiers
  • WOS:000372037500001
  • Scopus:84962566936
ISSN
2045-2322
DOI
10.1038/srep22883
language
English
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yes
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a7fd6fb0-1654-412a-b194-b3c70bad506f
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2016-04-12 15:04:17
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2016-11-14 09:42:44
@misc{a7fd6fb0-1654-412a-b194-b3c70bad506f,
  abstract     = {<p>Genetic and epigenetic factors may predispose women to polycystic ovary syndrome (PCOS), a common heritable disorder of unclear etiology. Here we investigated differences in genome-wide gene expression and DNA methylation in adipose tissue from 64 women with PCOS and 30 controls. In total, 1720 unique genes were differentially expressed (Q &lt; 0.05). Six out of twenty selected genes with largest expression difference (CYP1B1, GPT), genes linked to PCOS (RAB5B) or type 2 diabetes (PPARG, SVEP1), and methylation (DMAP1) were replicated in a separate case-control study. In total, 63,213 sites (P &lt; 0.05) and 440 sites (Q &lt; 0.15) were differently methylated. Thirty differentially expressed genes had corresponding changes in 33 different DNA methylation sites. Moreover, a total number of 1913 pairs of differentially expressed "gene-CpG" probes were significantly correlated after correction for multiple testing and corresponded with 349 unique genes. In conclusion, we identified a large number of genes and pathways that are affected in adipose tissue from women with PCOS. We also identified specific DNA methylation pathways that may affect mRNA expression. Together, these novel findings show that women with PCOS have multiple transcriptional and epigenetic changes in adipose tissue that are relevant for development of the disease.</p>},
  author       = {Kokosar, Milana and Benrick, Anna and Perfilyev, Alexander and Fornes, Romina and Nilsson, Emma A and Maliqueo, Manuel and Behre, Carl Johan and Sazonova, Antonina and Ohlsson, Claes and Ling, Charlotte and Stener-Victorin, Elisabet},
  issn         = {2045-2322},
  language     = {eng},
  publisher    = {ARRAY(0x9e07cf8)},
  series       = {Scientific Reports},
  title        = {Epigenetic and Transcriptional Alterations in Human Adipose Tissue of Polycystic Ovary Syndrome},
  url          = {http://dx.doi.org/10.1038/srep22883},
  volume       = {6},
  year         = {2016},
}