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Dose-dependent stimulation of bone induction by basic fibroblast growth factor in rats

Aspenberg, Per LU ; Thorngren, Karl Göran LU and Lohmander, L. Stefan LU (1991) In Acta Orthopaedica 62(5). p.481-484
Abstract

Implantation of demineralized bone matrix in rodents elicits a series of cellular events leading to the formation of new bone inside and adjacent to the implant. This process is believed to be initiated by an inductive protein present in bone matrix, and local growth factors may further regulate the process. We have previously shown that local application of recombinant human basic fibroblast growth factor (bFGF) in a carboxymethyl cellulose gel to demineralized bone matrix implants increases the bone yield as measured by calcium content 3 weeks after implantation in rats. We now report that this increase was seen at 3 and 4 weeks, but not earlier or later. Further, the stimulatory effect was seen with doses from 3 to 75 ng per implant.... (More)

Implantation of demineralized bone matrix in rodents elicits a series of cellular events leading to the formation of new bone inside and adjacent to the implant. This process is believed to be initiated by an inductive protein present in bone matrix, and local growth factors may further regulate the process. We have previously shown that local application of recombinant human basic fibroblast growth factor (bFGF) in a carboxymethyl cellulose gel to demineralized bone matrix implants increases the bone yield as measured by calcium content 3 weeks after implantation in rats. We now report that this increase was seen at 3 and 4 weeks, but not earlier or later. Further, the stimulatory effect was seen with doses from 3 to 75 ng per implant. A dose of 0.6 or 380 ng did not increase the bone yield, and 1,900 ng had a marked inhibitory effect. This narrow dosage optimum may reflect the complex actions of the growth factor. © 1991 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Acta Orthopaedica
volume
62
issue
5
pages
4 pages
publisher
Taylor & Francis
external identifiers
  • Scopus:0026014659
ISSN
1745-3674
DOI
10.3109/17453679108996650
language
English
LU publication?
no
id
b76a7f46-7f42-45c1-aa49-0588ecbb2966
date added to LUP
2016-05-04 18:19:22
date last changed
2016-11-08 15:05:27
@misc{b76a7f46-7f42-45c1-aa49-0588ecbb2966,
  abstract     = {<p>Implantation of demineralized bone matrix in rodents elicits a series of cellular events leading to the formation of new bone inside and adjacent to the implant. This process is believed to be initiated by an inductive protein present in bone matrix, and local growth factors may further regulate the process. We have previously shown that local application of recombinant human basic fibroblast growth factor (bFGF) in a carboxymethyl cellulose gel to demineralized bone matrix implants increases the bone yield as measured by calcium content 3 weeks after implantation in rats. We now report that this increase was seen at 3 and 4 weeks, but not earlier or later. Further, the stimulatory effect was seen with doses from 3 to 75 ng per implant. A dose of 0.6 or 380 ng did not increase the bone yield, and 1,900 ng had a marked inhibitory effect. This narrow dosage optimum may reflect the complex actions of the growth factor. © 1991 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.</p>},
  author       = {Aspenberg, Per and Thorngren, Karl Göran and Lohmander, L. Stefan},
  issn         = {1745-3674},
  language     = {eng},
  number       = {5},
  pages        = {481--484},
  publisher    = {ARRAY(0x9502c18)},
  series       = {Acta Orthopaedica},
  title        = {Dose-dependent stimulation of bone induction by basic fibroblast growth factor in rats},
  url          = {http://dx.doi.org/10.3109/17453679108996650},
  volume       = {62},
  year         = {1991},
}