Dose-dependent stimulation of bone induction by basic fibroblast growth factor in rats
Aspenberg, Per LU ; Thorngren, Karl Göran LU and Lohmander, L. Stefan LU
(1991)
In Acta Orthopaedica
62(5).
p.481-484
- Abstract
Implantation of demineralized bone matrix in rodents elicits a series of cellular events leading to the formation of new bone inside and adjacent to the implant. This process is believed to be initiated by an inductive protein present in bone matrix, and local growth factors may further regulate the process. We have previously shown that local application of recombinant human basic fibroblast growth factor (bFGF) in a carboxymethyl cellulose gel to demineralized bone matrix implants increases the bone yield as measured by calcium content 3 weeks after implantation in rats. We now report that this increase was seen at 3 and 4 weeks, but not earlier or later. Further, the stimulatory effect was seen with doses from 3 to 75 ng per implant.... (More)
Implantation of demineralized bone matrix in rodents elicits a series of cellular events leading to the formation of new bone inside and adjacent to the implant. This process is believed to be initiated by an inductive protein present in bone matrix, and local growth factors may further regulate the process. We have previously shown that local application of recombinant human basic fibroblast growth factor (bFGF) in a carboxymethyl cellulose gel to demineralized bone matrix implants increases the bone yield as measured by calcium content 3 weeks after implantation in rats. We now report that this increase was seen at 3 and 4 weeks, but not earlier or later. Further, the stimulatory effect was seen with doses from 3 to 75 ng per implant. A dose of 0.6 or 380 ng did not increase the bone yield, and 1,900 ng had a marked inhibitory effect. This narrow dosage optimum may reflect the complex actions of the growth factor.
(Less)
- author
- Aspenberg, Per
LU
; Thorngren, Karl Göran
LU
and Lohmander, L. Stefan
LU
- publishing date
- 1991
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Acta Orthopaedica
- volume
- 62
- issue
- 5
- pages
- 4 pages
- publisher
- Taylor & Francis
- external identifiers
-
- scopus:0026014659
- pmid:1835244
- ISSN
- 1745-3674
- DOI
- 10.3109/17453679108996650
- language
- English
- LU publication?
- no
- id
- b76a7f46-7f42-45c1-aa49-0588ecbb2966
- date added to LUP
- 2016-05-04 18:19:22
- date last changed
- 2024-01-04 02:47:15
@article{b76a7f46-7f42-45c1-aa49-0588ecbb2966,
abstract = {{<p>Implantation of demineralized bone matrix in rodents elicits a series of cellular events leading to the formation of new bone inside and adjacent to the implant. This process is believed to be initiated by an inductive protein present in bone matrix, and local growth factors may further regulate the process. We have previously shown that local application of recombinant human basic fibroblast growth factor (bFGF) in a carboxymethyl cellulose gel to demineralized bone matrix implants increases the bone yield as measured by calcium content 3 weeks after implantation in rats. We now report that this increase was seen at 3 and 4 weeks, but not earlier or later. Further, the stimulatory effect was seen with doses from 3 to 75 ng per implant. A dose of 0.6 or 380 ng did not increase the bone yield, and 1,900 ng had a marked inhibitory effect. This narrow dosage optimum may reflect the complex actions of the growth factor.</p>}},
author = {{Aspenberg, Per and Thorngren, Karl Göran and Lohmander, L. Stefan}},
issn = {{1745-3674}},
language = {{eng}},
number = {{5}},
pages = {{481--484}},
publisher = {{Taylor & Francis}},
series = {{Acta Orthopaedica}},
title = {{Dose-dependent stimulation of bone induction by basic fibroblast growth factor in rats}},
url = {{http://dx.doi.org/10.3109/17453679108996650}},
doi = {{10.3109/17453679108996650}},
volume = {{62}},
year = {{1991}},
}