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Biofilm formation enhances fomite survival of Streptococcus pneumoniae and Streptococcus pyogenes

Marks, Laura R; Reddinger, Ryan M and Hakansson, Anders P LU (2014) In Infection and Immunity 82(3). p.6-1141
Abstract

Both Streptococcus pyogenes and Streptococcus pneumoniae are widely thought to rapidly die outside the human host, losing infectivity following desiccation in the environment. However, to date, all literature investigating the infectivity of desiccated streptococci has used broth-grown, planktonic populations. In this study, we examined the impact of biofilm formation on environmental survival of clinical and laboratory isolates of S. pyogenes and S. pneumoniae as both organisms are thought to colonize the human host as biofilms. Results clearly demonstrate that while planktonic cells that are desiccated rapidly lose viability both on hands and abiotic surfaces, such as plastic, biofilm bacteria remain viable over extended periods of... (More)

Both Streptococcus pyogenes and Streptococcus pneumoniae are widely thought to rapidly die outside the human host, losing infectivity following desiccation in the environment. However, to date, all literature investigating the infectivity of desiccated streptococci has used broth-grown, planktonic populations. In this study, we examined the impact of biofilm formation on environmental survival of clinical and laboratory isolates of S. pyogenes and S. pneumoniae as both organisms are thought to colonize the human host as biofilms. Results clearly demonstrate that while planktonic cells that are desiccated rapidly lose viability both on hands and abiotic surfaces, such as plastic, biofilm bacteria remain viable over extended periods of time outside the host and remain infectious in a murine colonization model. To explore the level and extent of streptococcal fomite contamination that children might be exposed to naturally, direct bacteriologic cultures of items in a day care center were conducted, which demonstrated high levels of viable streptococci of both species. These findings raise the possibility that streptococci may survive in the environment and be transferred from person to person via fomites contaminated with oropharyngeal secretions containing biofilm streptococci.

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author
publishing date
type
Contribution to journal
publication status
published
keywords
Animals, Biofilms, Cell Line, Tumor, Cells, Cultured, Female, Fomites, Humans, Mice, Mice, Inbred BALB C, Streptococcus pneumoniae, Streptococcus pyogenes
in
Infection and Immunity
volume
82
issue
3
pages
6 pages
publisher
American Society for Microbiology
external identifiers
  • Scopus:84894227485
ISSN
1098-5522
DOI
10.1128/IAI.01310-13
language
English
LU publication?
no
id
bf4f28fa-9be8-4f88-ba64-48f89fb4fed5
date added to LUP
2016-05-21 10:48:13
date last changed
2016-11-20 04:33:46
@misc{bf4f28fa-9be8-4f88-ba64-48f89fb4fed5,
  abstract     = {<p>Both Streptococcus pyogenes and Streptococcus pneumoniae are widely thought to rapidly die outside the human host, losing infectivity following desiccation in the environment. However, to date, all literature investigating the infectivity of desiccated streptococci has used broth-grown, planktonic populations. In this study, we examined the impact of biofilm formation on environmental survival of clinical and laboratory isolates of S. pyogenes and S. pneumoniae as both organisms are thought to colonize the human host as biofilms. Results clearly demonstrate that while planktonic cells that are desiccated rapidly lose viability both on hands and abiotic surfaces, such as plastic, biofilm bacteria remain viable over extended periods of time outside the host and remain infectious in a murine colonization model. To explore the level and extent of streptococcal fomite contamination that children might be exposed to naturally, direct bacteriologic cultures of items in a day care center were conducted, which demonstrated high levels of viable streptococci of both species. These findings raise the possibility that streptococci may survive in the environment and be transferred from person to person via fomites contaminated with oropharyngeal secretions containing biofilm streptococci.</p>},
  author       = {Marks, Laura R and Reddinger, Ryan M and Hakansson, Anders P},
  issn         = {1098-5522},
  keyword      = {Animals,Biofilms,Cell Line, Tumor,Cells, Cultured,Female,Fomites,Humans,Mice,Mice, Inbred BALB C,Streptococcus pneumoniae,Streptococcus pyogenes},
  language     = {eng},
  number       = {3},
  pages        = {6--1141},
  publisher    = {ARRAY(0x9d98960)},
  series       = {Infection and Immunity},
  title        = {Biofilm formation enhances fomite survival of Streptococcus pneumoniae and Streptococcus pyogenes},
  url          = {http://dx.doi.org/10.1128/IAI.01310-13},
  volume       = {82},
  year         = {2014},
}