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Stromelysin, tissue inhibitor of metalloproteinases and proteoglycan fragments in human knee joint fluid after injury

Lohmander, L. S. LU ; Hoerrner, L. A.; Dahlberg, L. LU ; Roos, H. LU ; Bjornsson, S. and Lark, M. W. (1993) In Journal of Rheumatology 20(8). p.1362-1368
Abstract

Objective. To determine in a cross sectional study the concentrations of stromelysin, tissue inhibitor of metalloproteinases (TIMP), and proteoglycan fragments in knee synovial fluid (SF) at different times after injury to cruciate ligament or meniscus. Methods. Joint fluid samples were obtained from patients with knee injury diagnosed by arthroscopy. Concentrations of stromelysin-1 and TIMP-1 were determined by immunoassay with monoclonal and polyclonal antibodies. Cartilage proteoglycan fragments were quantified by immunoassay with polyclonal antibodies or by dye precipitation. Results. Average concentrations of stromelysin increased 40-fold in association with injury, and after about 6 months decreased to a plateau level about... (More)

Objective. To determine in a cross sectional study the concentrations of stromelysin, tissue inhibitor of metalloproteinases (TIMP), and proteoglycan fragments in knee synovial fluid (SF) at different times after injury to cruciate ligament or meniscus. Methods. Joint fluid samples were obtained from patients with knee injury diagnosed by arthroscopy. Concentrations of stromelysin-1 and TIMP-1 were determined by immunoassay with monoclonal and polyclonal antibodies. Cartilage proteoglycan fragments were quantified by immunoassay with polyclonal antibodies or by dye precipitation. Results. Average concentrations of stromelysin increased 40-fold in association with injury, and after about 6 months decreased to a plateau level about 10-fold increased compared to a reference group with healthy knees. TIMP and proteoglycan levels also increased in similar temporal patterns, but less markedly. Increased average SF levels of these markers were maintained for at least 17 years after injury. SF from knees with injury contained a 1.5 to 2.5 molar excess of stromelysin over TIMP, while reference joint fluids contained a 2-fold molar excess of TIMP over stromelysin. Conclusion. The persistent changes in SF markers after joint injury may be associated with the cartilage destruction and frequent development of posttraumatic osteoarthritis in this group of patients.

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author
organization
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type
Contribution to journal
publication status
published
subject
keywords
INJURY, JOINT CARTILAGE, METALLOPROTEINASE, OSTEOARTHRITIS, SYNOVIAL FLUID
in
Journal of Rheumatology
volume
20
issue
8
pages
7 pages
publisher
J Rheumatol Publ Co
external identifiers
  • Scopus:0027427719
ISSN
0315-162X
language
English
LU publication?
yes
id
c56fb193-8886-4dc3-8a53-a49c0f309150
date added to LUP
2016-05-04 18:27:59
date last changed
2016-08-02 16:04:20
@misc{c56fb193-8886-4dc3-8a53-a49c0f309150,
  abstract     = {<p>Objective. To determine in a cross sectional study the concentrations of stromelysin, tissue inhibitor of metalloproteinases (TIMP), and proteoglycan fragments in knee synovial fluid (SF) at different times after injury to cruciate ligament or meniscus. Methods. Joint fluid samples were obtained from patients with knee injury diagnosed by arthroscopy. Concentrations of stromelysin-1 and TIMP-1 were determined by immunoassay with monoclonal and polyclonal antibodies. Cartilage proteoglycan fragments were quantified by immunoassay with polyclonal antibodies or by dye precipitation. Results. Average concentrations of stromelysin increased 40-fold in association with injury, and after about 6 months decreased to a plateau level about 10-fold increased compared to a reference group with healthy knees. TIMP and proteoglycan levels also increased in similar temporal patterns, but less markedly. Increased average SF levels of these markers were maintained for at least 17 years after injury. SF from knees with injury contained a 1.5 to 2.5 molar excess of stromelysin over TIMP, while reference joint fluids contained a 2-fold molar excess of TIMP over stromelysin. Conclusion. The persistent changes in SF markers after joint injury may be associated with the cartilage destruction and frequent development of posttraumatic osteoarthritis in this group of patients.</p>},
  author       = {Lohmander, L. S. and Hoerrner, L. A. and Dahlberg, L. and Roos, H. and Bjornsson, S. and Lark, M. W.},
  issn         = {0315-162X},
  keyword      = {INJURY,JOINT CARTILAGE,METALLOPROTEINASE,OSTEOARTHRITIS,SYNOVIAL FLUID},
  language     = {eng},
  number       = {8},
  pages        = {1362--1368},
  publisher    = {ARRAY(0x92b4468)},
  series       = {Journal of Rheumatology},
  title        = {Stromelysin, tissue inhibitor of metalloproteinases and proteoglycan fragments in human knee joint fluid after injury},
  volume       = {20},
  year         = {1993},
}