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Xyloside-primed chondroitin sulfate/dermatan sulfate from breast carcinoma cells with a defined disaccharide composition has cytotoxic effects in vitro

Persson, Andrea LU ; Tykesson, Emil LU ; Westergren-Thorsson, Gunilla LU ; Malmström, Anders LU ; Ellervik, Ulf LU and Mani, Katrin LU (2016) In Journal of Biological Chemistry 291(28). p.14871-14882
Abstract

We have previously reported that the xyloside 2-(6-hydroxynaphthyl) β-D-xylopyranoside (XylNapOH), in contrast to 2-naphthyl β-D-xylopyranoside (XylNap), specifically reduces tumor growth both in vitro and in vivo. Although there are indications that this could be mediated by the xyloside-primed glycosaminoglycans (GAGs) and that these differ in composition depending on xyloside and cell type, detailed knowledge regarding a structure-function relationship is lacking. In this study, we isolated XylNapOH- and XylNap-primed GAGs from a breast carcinoma cell line, HCC70, and a breast fibroblast cell line, CCD-1095Sk, and demonstrated that both XylNapOH- and XylNap-primed chondroitin sulfate/dermatan sulfate (CS/DS) GAGs derived from HCC70... (More)

We have previously reported that the xyloside 2-(6-hydroxynaphthyl) β-D-xylopyranoside (XylNapOH), in contrast to 2-naphthyl β-D-xylopyranoside (XylNap), specifically reduces tumor growth both in vitro and in vivo. Although there are indications that this could be mediated by the xyloside-primed glycosaminoglycans (GAGs) and that these differ in composition depending on xyloside and cell type, detailed knowledge regarding a structure-function relationship is lacking. In this study, we isolated XylNapOH- and XylNap-primed GAGs from a breast carcinoma cell line, HCC70, and a breast fibroblast cell line, CCD-1095Sk, and demonstrated that both XylNapOH- and XylNap-primed chondroitin sulfate/dermatan sulfate (CS/DS) GAGs derived from HCC70 cells had a cytotoxic effect on HCC70 cells and CCD-1095Sk cells. The cytotoxic effect appeared to be mediated by induction of apoptosis and was inhibited in a concentration-dependent manner by the XylNap-primed heparan sulfate (HS) GAGs. In contrast, neither the CS/DS nor the HS derived from CCD-1095Sk cells primed on XylNapOH or XylNap had any effect on the growth of HCC70 cells or CCD-105Sk cells. These observations were related to the disaccharide composition of the XylNapOH- and XylNap-primed GAGs, which differed considerably between the two cell lines, but was similar when the GAGs were derived from the same cell line. To our knowledge, this is the first report on cytotoxic effects mediated by CS/DS.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Biological Chemistry
volume
291
issue
28
pages
14871 - 14882
publisher
American Society for Biochemistry and Molecular Biology
external identifiers
  • Scopus:84978999372
ISSN
1083-351X
DOI
10.1074/jbc.M116.716829
language
English
LU publication?
yes
id
cad5b9c1-847b-4b81-9f15-0bb77f3ec537
date added to LUP
2016-06-08 11:41:02
date last changed
2016-10-23 04:43:49
@misc{cad5b9c1-847b-4b81-9f15-0bb77f3ec537,
  abstract     = {<p>We have previously reported that the xyloside 2-(6-hydroxynaphthyl) β-D-xylopyranoside (XylNapOH), in contrast to 2-naphthyl β-D-xylopyranoside (XylNap), specifically reduces tumor growth both in vitro and in vivo. Although there are indications that this could be mediated by the xyloside-primed glycosaminoglycans (GAGs) and that these differ in composition depending on xyloside and cell type, detailed knowledge regarding a structure-function relationship is lacking. In this study, we isolated XylNapOH- and XylNap-primed GAGs from a breast carcinoma cell line, HCC70, and a breast fibroblast cell line, CCD-1095Sk, and demonstrated that both XylNapOH- and XylNap-primed chondroitin sulfate/dermatan sulfate (CS/DS) GAGs derived from HCC70 cells had a cytotoxic effect on HCC70 cells and CCD-1095Sk cells. The cytotoxic effect appeared to be mediated by induction of apoptosis and was inhibited in a concentration-dependent manner by the XylNap-primed heparan sulfate (HS) GAGs. In contrast, neither the CS/DS nor the HS derived from CCD-1095Sk cells primed on XylNapOH or XylNap had any effect on the growth of HCC70 cells or CCD-105Sk cells. These observations were related to the disaccharide composition of the XylNapOH- and XylNap-primed GAGs, which differed considerably between the two cell lines, but was similar when the GAGs were derived from the same cell line. To our knowledge, this is the first report on cytotoxic effects mediated by CS/DS.</p>},
  author       = {Persson, Andrea and Tykesson, Emil and Westergren-Thorsson, Gunilla and Malmström, Anders and Ellervik, Ulf and Mani, Katrin},
  issn         = {1083-351X},
  language     = {eng},
  number       = {28},
  pages        = {14871--14882},
  publisher    = {ARRAY(0xe340e58)},
  series       = {Journal of Biological Chemistry},
  title        = {Xyloside-primed chondroitin sulfate/dermatan sulfate from breast carcinoma cells with a defined disaccharide composition has cytotoxic effects in vitro},
  url          = {http://dx.doi.org/10.1074/jbc.M116.716829},
  volume       = {291},
  year         = {2016},
}