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Is nitric oxide (NO) produced by invertebrate neurons?

Elofsson, Rolf LU ; Carlberg, Mats; Moroz, Leonid; Nezlin, Leonid and Sakharov, Dmitri A (1993) In NeuroReport 4. p.279-282
Abstract
NADPH-DIAPHORASE (NADPHd) is known to be identical to nitric oxide (NO) synthase in the mammalian nervous system, and is therefore used as a marker of NO-producing neurones. Using the histochemical reaction for NADPHd, we searched for such neurones in a selection of invertebrates. Special emphasis was given to molluscs. No selective neuronal staining was found in representatives of coelenterates, turbellarians, nematodes and urochordates. In all annelids, arthropods and molluscs examined, with the exception of a chiton, specific neurones were selectively stained. The reaction was particularly strong in pulmonate molluscs where scattered positive neurones were found in various ganglia and clustered symmetrically in the paired buccal... (More)
NADPH-DIAPHORASE (NADPHd) is known to be identical to nitric oxide (NO) synthase in the mammalian nervous system, and is therefore used as a marker of NO-producing neurones. Using the histochemical reaction for NADPHd, we searched for such neurones in a selection of invertebrates. Special emphasis was given to molluscs. No selective neuronal staining was found in representatives of coelenterates, turbellarians, nematodes and urochordates. In all annelids, arthropods and molluscs examined, with the exception of a chiton, specific neurones were selectively stained. The reaction was particularly strong in pulmonate molluscs where scattered positive neurones were found in various ganglia and clustered symmetrically in the paired buccal ganglia. Biochemical assay of NO synthase in osphradia of the gastropod mollusc Lymnaea stagnalis revealed a formation of citrullin that was inhibited by the specific NO synthase N[omega]-nitro-L-arginine (NO2Arg). Both histochemical and biochemical methods indicate that NO can be used as a signal molecule by specific neurones in advanced invertebrates. (Less)
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Contribution to journal
publication status
published
subject
keywords
NADPH-diaphorase
in
NeuroReport
volume
4
pages
279 - 282
publisher
Lippincott Williams & Wilkins
ISSN
1473-558X
language
English
LU publication?
yes
id
ce774af1-0cc5-4ea9-b93b-827de1e1d36d
date added to LUP
2016-11-03 19:16:52
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2016-11-21 14:25:12
@misc{ce774af1-0cc5-4ea9-b93b-827de1e1d36d,
  abstract     = {NADPH-DIAPHORASE (NADPHd) is known to be identical to nitric oxide (NO) synthase in the mammalian nervous system, and is therefore used as a marker of NO-producing neurones. Using the histochemical reaction for NADPHd, we searched for such neurones in a selection of invertebrates. Special emphasis was given to molluscs. No selective neuronal staining was found in representatives of coelenterates, turbellarians, nematodes and urochordates. In all annelids, arthropods and molluscs examined, with the exception of a chiton, specific neurones were selectively stained. The reaction was particularly strong in pulmonate molluscs where scattered positive neurones were found in various ganglia and clustered symmetrically in the paired buccal ganglia. Biochemical assay of NO synthase in osphradia of the gastropod mollusc Lymnaea stagnalis revealed a formation of citrullin that was inhibited by the specific NO synthase N[omega]-nitro-L-arginine (NO2Arg). Both histochemical and biochemical methods indicate that NO can be used as a signal molecule by specific neurones in advanced invertebrates.},
  author       = {Elofsson, Rolf and Carlberg, Mats and Moroz, Leonid and Nezlin, Leonid and Sakharov, Dmitri A },
  issn         = {1473-558X},
  keyword      = {NADPH-diaphorase},
  language     = {eng},
  pages        = {279--282},
  publisher    = {ARRAY(0xd61b020)},
  series       = {NeuroReport},
  title        = {Is nitric oxide (NO) produced by invertebrate neurons?},
  volume       = {4},
  year         = {1993},
}