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Performance of two immunoassays for the determination of atrazine in sea water samples as compared with on-line solid phase extraction-liquid chromatography-diode array detection

Gascón, Jordi; Oubiña, Anna; Ferrer, Imma; Önnerfjord, Patrik LU ; Marko-Varga, György LU ; Hammock, Bruce D.; Marco, Maria Pilar and Barceló, Damià (1996) In Analytica Chimica Acta 330(1). p.41-51
Abstract

Two immunoassay formats, magnetic particles-based assay (Atrazine RaPID assay and Atrazine High-Sensitivity RaPID assay) and microtiter plate based assay (Department of Entomology and Environmental Toxicology, University of California in Davis) were evaluated for the determination of atrazine in sea water samples. The results obtained were compared and validated with those obtained by using on-line solid phase extraction followed by liquid chromatography-diode array detection (on-line SPE-LC-DAD). The correlation between both techniques was good when analyzing levels of atrazine ranging from 0.01 to 5 μg/l in samples showing salt concentration values varying from 0 to 35 g/l and pH values from 2 to 10. One of these immunoassays... (More)

Two immunoassay formats, magnetic particles-based assay (Atrazine RaPID assay and Atrazine High-Sensitivity RaPID assay) and microtiter plate based assay (Department of Entomology and Environmental Toxicology, University of California in Davis) were evaluated for the determination of atrazine in sea water samples. The results obtained were compared and validated with those obtained by using on-line solid phase extraction followed by liquid chromatography-diode array detection (on-line SPE-LC-DAD). The correlation between both techniques was good when analyzing levels of atrazine ranging from 0.01 to 5 μg/l in samples showing salt concentration values varying from 0 to 35 g/l and pH values from 2 to 10. One of these immunoassays (Atrazine High-Sensitivity RaPID assay) was employed to directly analyze atrazine in real estuarine and coastal water samples. The same samples were analyzed after filtration and C18 Empore disks extraction.

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Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
keywords
Atrazine, Chromatography, Immunoassay, Sea water, Solid-phase extraction, Waters
in
Analytica Chimica Acta
volume
330
issue
1
pages
41 - 51
publisher
Elsevier
external identifiers
  • Scopus:0030606899
ISSN
0003-2670
DOI
10.1016/0003-2670(96)00143-2
language
English
LU publication?
yes
id
d54d0add-cb22-4075-ac26-eaaec33c07ee
date added to LUP
2016-10-14 11:38:45
date last changed
2016-11-29 08:58:49
@misc{d54d0add-cb22-4075-ac26-eaaec33c07ee,
  abstract     = {<p>Two immunoassay formats, magnetic particles-based assay (Atrazine RaPID assay and Atrazine High-Sensitivity RaPID assay) and microtiter plate based assay (Department of Entomology and Environmental Toxicology, University of California in Davis) were evaluated for the determination of atrazine in sea water samples. The results obtained were compared and validated with those obtained by using on-line solid phase extraction followed by liquid chromatography-diode array detection (on-line SPE-LC-DAD). The correlation between both techniques was good when analyzing levels of atrazine ranging from 0.01 to 5 μg/l in samples showing salt concentration values varying from 0 to 35 g/l and pH values from 2 to 10. One of these immunoassays (Atrazine High-Sensitivity RaPID assay) was employed to directly analyze atrazine in real estuarine and coastal water samples. The same samples were analyzed after filtration and C<sub>18</sub> Empore disks extraction.</p>},
  author       = {Gascón, Jordi and Oubiña, Anna and Ferrer, Imma and Önnerfjord, Patrik and Marko-Varga, György and Hammock, Bruce D. and Marco, Maria Pilar and Barceló, Damià},
  issn         = {0003-2670},
  keyword      = {Atrazine,Chromatography,Immunoassay,Sea water,Solid-phase extraction,Waters},
  language     = {eng},
  month        = {08},
  number       = {1},
  pages        = {41--51},
  publisher    = {ARRAY(0xabc0008)},
  series       = {Analytica Chimica Acta},
  title        = {Performance of two immunoassays for the determination of atrazine in sea water samples as compared with on-line solid phase extraction-liquid chromatography-diode array detection},
  url          = {http://dx.doi.org/10.1016/0003-2670(96)00143-2},
  volume       = {330},
  year         = {1996},
}