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A wide spectrum of fastidious and ampicillin-susceptible bacteria dominate in animal-caused wounds

Gustavsson, O.; Johansson, A. V.; Monstein, H. J.; Nilsson, L. E. and Bredberg, A. LU (2016) In European Journal of Clinical Microbiology & Infectious Diseases 35(8). p.1315-1321
Abstract

The main purpose of this study was to assess the actual occurrence of Gram-negative oxidase-positive bacteria (GNOP) in human wounds caused by animals, mostly cat and dog bites and scratches, and with signs of infection. We report a prospective series of 92 wound samples. Routine culturing was combined with a procedure optimised for fastidious GNOP. All GNOP isolates were identified by 16S rDNA sequencing to the species level. We observed a more prominent role of GNOP, including at least 30 species mostly in the families Flavobacteriaceae, Neisseriaceae and Pasteurellaceae, and less of Staphylococcus aureus and streptococci. The antibiotic susceptibility pattern was investigated, as GNOP are associated with sudden onset of serious... (More)

The main purpose of this study was to assess the actual occurrence of Gram-negative oxidase-positive bacteria (GNOP) in human wounds caused by animals, mostly cat and dog bites and scratches, and with signs of infection. We report a prospective series of 92 wound samples. Routine culturing was combined with a procedure optimised for fastidious GNOP. All GNOP isolates were identified by 16S rDNA sequencing to the species level. We observed a more prominent role of GNOP, including at least 30 species mostly in the families Flavobacteriaceae, Neisseriaceae and Pasteurellaceae, and less of Staphylococcus aureus and streptococci. The antibiotic susceptibility pattern was investigated, as GNOP are associated with sudden onset of serious infections, making an early decision on antibiotic treatment vital. All GNOP isolates judged to be clinically relevant displayed susceptibility to ampicillin and meropenem, but resistance to oxacillin, clindamycin and gentamicin was frequent. Our findings emphasise the need to cover GNOP as recommended in guidelines, and not only common wound pathogens, when treating an animal-caused wound.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
European Journal of Clinical Microbiology & Infectious Diseases
volume
35
issue
8
pages
7 pages
publisher
Springer
external identifiers
  • Scopus:84969754391
ISSN
0934-9723
DOI
10.1007/s10096-016-2667-z
language
English
LU publication?
yes
id
e57c3724-1dd8-4f38-ab8c-a16b87b906ca
date added to LUP
2016-06-17 17:13:18
date last changed
2016-10-18 09:53:20
@misc{e57c3724-1dd8-4f38-ab8c-a16b87b906ca,
  abstract     = {<p>The main purpose of this study was to assess the actual occurrence of Gram-negative oxidase-positive bacteria (GNOP) in human wounds caused by animals, mostly cat and dog bites and scratches, and with signs of infection. We report a prospective series of 92 wound samples. Routine culturing was combined with a procedure optimised for fastidious GNOP. All GNOP isolates were identified by 16S rDNA sequencing to the species level. We observed a more prominent role of GNOP, including at least 30 species mostly in the families Flavobacteriaceae, Neisseriaceae and Pasteurellaceae, and less of Staphylococcus aureus and streptococci. The antibiotic susceptibility pattern was investigated, as GNOP are associated with sudden onset of serious infections, making an early decision on antibiotic treatment vital. All GNOP isolates judged to be clinically relevant displayed susceptibility to ampicillin and meropenem, but resistance to oxacillin, clindamycin and gentamicin was frequent. Our findings emphasise the need to cover GNOP as recommended in guidelines, and not only common wound pathogens, when treating an animal-caused wound.</p>},
  author       = {Gustavsson, O. and Johansson, A. V. and Monstein, H. J. and Nilsson, L. E. and Bredberg, A.},
  issn         = {0934-9723},
  language     = {eng},
  number       = {8},
  pages        = {1315--1321},
  publisher    = {ARRAY(0xaeb5028)},
  series       = {European Journal of Clinical Microbiology & Infectious Diseases},
  title        = {A wide spectrum of fastidious and ampicillin-susceptible bacteria dominate in animal-caused wounds},
  url          = {http://dx.doi.org/10.1007/s10096-016-2667-z},
  volume       = {35},
  year         = {2016},
}