Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Concurrent acquired inhibitors to factor VIII and IX, a laboratory artifact : A case report

Anžej Doma, Sasa ; Hillarp, Andreas LU ; Pajič, Tadey ; Andoljšek, Dusan ; Černelč, Peter and Preložnik Zupan, Irena (2016) In Biochemia Medica 26(2). p.279-284
Abstract

Acquired inhibitors to coagulation factors other than factor VIII are extremely rare. We describe a case of a 59-year-old woman with abnormal bleeding, diagnosed with concurrent inhibitor antibodies to factor VIII and IX by Bethesda testing. We demonstrate that anti-FVIII antibodies of a very high titre are capable of disturbing the aPTT-based Bethesda assay, resulting in falsely-positive antibodies to factor IX. The case also illustrates the usefulness of the immunological assay (ELISA) in complementing the inhibitor diagnosis.

Please use this url to cite or link to this publication:
author
; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Autoantibody, Blood coagulation factor, Enzyme-linked immunosorbent assay, Inhibitor
in
Biochemia Medica
volume
26
issue
2
pages
279 - 284
publisher
Biochemia Medica, Editorial Office
external identifiers
  • scopus:84973456477
  • pmid:27346976
  • wos:000377907400020
ISSN
1330-0962
DOI
10.11613/BM.2016.033
language
English
LU publication?
no
id
e61fc36b-e931-4094-9f44-66b768544295
date added to LUP
2016-07-12 15:02:48
date last changed
2024-03-07 09:33:55
@article{e61fc36b-e931-4094-9f44-66b768544295,
  abstract     = {{<p>Acquired inhibitors to coagulation factors other than factor VIII are extremely rare. We describe a case of a 59-year-old woman with abnormal bleeding, diagnosed with concurrent inhibitor antibodies to factor VIII and IX by Bethesda testing. We demonstrate that anti-FVIII antibodies of a very high titre are capable of disturbing the aPTT-based Bethesda assay, resulting in falsely-positive antibodies to factor IX. The case also illustrates the usefulness of the immunological assay (ELISA) in complementing the inhibitor diagnosis.</p>}},
  author       = {{Anžej Doma, Sasa and Hillarp, Andreas and Pajič, Tadey and Andoljšek, Dusan and Černelč, Peter and Preložnik Zupan, Irena}},
  issn         = {{1330-0962}},
  keywords     = {{Autoantibody; Blood coagulation factor; Enzyme-linked immunosorbent assay; Inhibitor}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{279--284}},
  publisher    = {{Biochemia Medica, Editorial Office}},
  series       = {{Biochemia Medica}},
  title        = {{Concurrent acquired inhibitors to factor VIII and IX, a laboratory artifact : A case report}},
  url          = {{http://dx.doi.org/10.11613/BM.2016.033}},
  doi          = {{10.11613/BM.2016.033}},
  volume       = {{26}},
  year         = {{2016}},
}