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Altered gene expression associated with apoptosis in a pre-B-leukemic cell line following cross-linking of MHC class I

Nilsson, Nina LU ; Wallén-Öhman, Marie; Ohlin, Mats LU and Borrebaeck, C. A K LU (1997) In Experimental Cell Research 231(1). p.190-197
Abstract

The major histocompatibility complex class I (MHC-I) has recently been shown not only to present antigens to the immune system but also to mediate transmembrane signaling, resulting in activation, inactivation, or apoptosis. Such signaling has been observed in both normal and malignant cells of the B and T cell lineage. Cross-linking of MHC-I on the pre-B- acute-lymphocytic cell line KM-3 induces an apoptotic process, which becomes evident after approximately 12 h. In order to better understand the mechanisms regulating this apoptotic process, we have investigated both gene expression and the effect of cross-linking on certain intracellular events. Differential display PCR was used to isolate two gene fragments whose level of expression... (More)

The major histocompatibility complex class I (MHC-I) has recently been shown not only to present antigens to the immune system but also to mediate transmembrane signaling, resulting in activation, inactivation, or apoptosis. Such signaling has been observed in both normal and malignant cells of the B and T cell lineage. Cross-linking of MHC-I on the pre-B- acute-lymphocytic cell line KM-3 induces an apoptotic process, which becomes evident after approximately 12 h. In order to better understand the mechanisms regulating this apoptotic process, we have investigated both gene expression and the effect of cross-linking on certain intracellular events. Differential display PCR was used to isolate two gene fragments whose level of expression was associated with the induction of apoptosis as they were downregulated in KM-3 cells following MHC-I cross-linking. These genes encode novel molecules whose function remains to be elucidated. It was further demonstrated that the apoptotic process was not accompanied by changes in [Ca2+](i), the level of activation of NF-κB, or changes in protein kinase C activity and that the initiation of apoptosis could be prevented by phorbol ester treatment. It is thus suggested that multiple, fine-tuned molecular events determine the outcome of cross-linking of MHC-I in this pre-B-lymphocytic cell line.

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author
organization
publishing date
type
Contribution to journal
publication status
published
in
Experimental Cell Research
volume
231
issue
1
pages
8 pages
publisher
Academic Press
external identifiers
  • Scopus:0031585863
ISSN
0014-4827
DOI
10.1006/excr.1996.3454
language
English
LU publication?
yes
id
ebd7ca8f-4384-4f6c-9534-75200537ab12
date added to LUP
2016-04-19 14:07:08
date last changed
2016-04-22 12:54:52
@misc{ebd7ca8f-4384-4f6c-9534-75200537ab12,
  abstract     = {<p>The major histocompatibility complex class I (MHC-I) has recently been shown not only to present antigens to the immune system but also to mediate transmembrane signaling, resulting in activation, inactivation, or apoptosis. Such signaling has been observed in both normal and malignant cells of the B and T cell lineage. Cross-linking of MHC-I on the pre-B- acute-lymphocytic cell line KM-3 induces an apoptotic process, which becomes evident after approximately 12 h. In order to better understand the mechanisms regulating this apoptotic process, we have investigated both gene expression and the effect of cross-linking on certain intracellular events. Differential display PCR was used to isolate two gene fragments whose level of expression was associated with the induction of apoptosis as they were downregulated in KM-3 cells following MHC-I cross-linking. These genes encode novel molecules whose function remains to be elucidated. It was further demonstrated that the apoptotic process was not accompanied by changes in [Ca<sup>2+</sup>](i), the level of activation of NF-κB, or changes in protein kinase C activity and that the initiation of apoptosis could be prevented by phorbol ester treatment. It is thus suggested that multiple, fine-tuned molecular events determine the outcome of cross-linking of MHC-I in this pre-B-lymphocytic cell line.</p>},
  author       = {Nilsson, Nina and Wallén-Öhman, Marie and Ohlin, Mats and Borrebaeck, C. A K},
  issn         = {0014-4827},
  language     = {eng},
  month        = {02},
  number       = {1},
  pages        = {190--197},
  publisher    = {ARRAY(0x8aa4e18)},
  series       = {Experimental Cell Research},
  title        = {Altered gene expression associated with apoptosis in a pre-B-leukemic cell line following cross-linking of MHC class I},
  url          = {http://dx.doi.org/10.1006/excr.1996.3454},
  volume       = {231},
  year         = {1997},
}