Advanced

Plasma proteomics in CML patients before and after initiation of tyrosine kinase inhibitor therapy reveals induced Th1 immunity and loss of angiogenic stimuli

Söderlund, Stina; Christiansson, Lisa; Persson, Inger; Hjorth-Hansen, Henrik; Richter, Johan LU ; Simonsson, Bengt; Mustjoki, Satu; Olsson-Strömberg, Ulla and Loskog, Angelica (2016) In Leukemia Research 50. p.95-103
Abstract

Background and aims The simultaneous measurement of many proteins is now possible using multiplex assays. In this pilot study we investigated a total of 124 proteins in plasma from chronic myeloid leukemia (CML) patients with the purpose of identifying proteins that are differently expressed at diagnosis and after tyrosine kinase inhibitor (TKI) treatment initiation. Methods Samples were taken from 14 CML patients at diagnosis and after three months of TKI treatment (imatinib or dasatinib). Samples were analyzed by Mesoscale Discovery, Myriad RBM MAP technology and Olink Proseek. Results Multiple plasma proteins were differentially expressed before and after initiation of TKI therapy. Protein patterns demonstrated a possible shift... (More)

Background and aims The simultaneous measurement of many proteins is now possible using multiplex assays. In this pilot study we investigated a total of 124 proteins in plasma from chronic myeloid leukemia (CML) patients with the purpose of identifying proteins that are differently expressed at diagnosis and after tyrosine kinase inhibitor (TKI) treatment initiation. Methods Samples were taken from 14 CML patients at diagnosis and after three months of TKI treatment (imatinib or dasatinib). Samples were analyzed by Mesoscale Discovery, Myriad RBM MAP technology and Olink Proseek. Results Multiple plasma proteins were differentially expressed before and after initiation of TKI therapy. Protein patterns demonstrated a possible shift towards Th1-immunity and reduced angiogenic stimuli. Further, some plasma proteins were identified that can be of potential interest to study further for biologic, prognostic or therapeutic significance such as E-selectin, uPAR, growth hormone and carbonic anhydrase IX. Conclusions Plasma proteomics seems feasible and useful in CML patients, both for studying patterns of protein expression and for identifying single proteins differentially expressed before and after treatment. Plasma proteomics may be useful to map disease activity and biological processes. Hence, plasma proteomics can be used to understand drug mechanisms and treatment responses in CML.

(Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Angiogenesis, Chronic myeloid leukemia, Proteomics, Th1, Tyrosine kinase inhibitor
in
Leukemia Research
volume
50
pages
9 pages
publisher
Elsevier
external identifiers
  • Scopus:84989344889
ISSN
0145-2126
DOI
10.1016/j.leukres.2016.09.019
language
English
LU publication?
yes
id
f140f042-d9bd-4b77-ba2a-e4f973477e3e
date added to LUP
2016-10-18 12:03:13
date last changed
2016-11-08 07:47:50
@misc{f140f042-d9bd-4b77-ba2a-e4f973477e3e,
  abstract     = {<p>Background and aims The simultaneous measurement of many proteins is now possible using multiplex assays. In this pilot study we investigated a total of 124 proteins in plasma from chronic myeloid leukemia (CML) patients with the purpose of identifying proteins that are differently expressed at diagnosis and after tyrosine kinase inhibitor (TKI) treatment initiation. Methods Samples were taken from 14 CML patients at diagnosis and after three months of TKI treatment (imatinib or dasatinib). Samples were analyzed by Mesoscale Discovery, Myriad RBM MAP technology and Olink Proseek. Results Multiple plasma proteins were differentially expressed before and after initiation of TKI therapy. Protein patterns demonstrated a possible shift towards Th1-immunity and reduced angiogenic stimuli. Further, some plasma proteins were identified that can be of potential interest to study further for biologic, prognostic or therapeutic significance such as E-selectin, uPAR, growth hormone and carbonic anhydrase IX. Conclusions Plasma proteomics seems feasible and useful in CML patients, both for studying patterns of protein expression and for identifying single proteins differentially expressed before and after treatment. Plasma proteomics may be useful to map disease activity and biological processes. Hence, plasma proteomics can be used to understand drug mechanisms and treatment responses in CML.</p>},
  author       = {Söderlund, Stina and Christiansson, Lisa and Persson, Inger and Hjorth-Hansen, Henrik and Richter, Johan and Simonsson, Bengt and Mustjoki, Satu and Olsson-Strömberg, Ulla and Loskog, Angelica},
  issn         = {0145-2126},
  keyword      = {Angiogenesis,Chronic myeloid leukemia,Proteomics,Th1,Tyrosine kinase inhibitor},
  language     = {eng},
  month        = {11},
  pages        = {95--103},
  publisher    = {ARRAY(0xa0ddb60)},
  series       = {Leukemia Research},
  title        = {Plasma proteomics in CML patients before and after initiation of tyrosine kinase inhibitor therapy reveals induced Th1 immunity and loss of angiogenic stimuli},
  url          = {http://dx.doi.org/10.1016/j.leukres.2016.09.019},
  volume       = {50},
  year         = {2016},
}