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Seeing through the interface : Poly(ε-Caprolactone) surface modification of poly(glycerol-co-sebacic acid) membranes in adult porcine retinal explants

Taylor, Linnéa LU ; Arnér, Karin LU ; Kolewe, Martin; Pritchard, Christopher; Hendy, Gillian; Langer, Robert and Ghosh, Fredrik LU (2016) In Journal of Tissue Engineering and Regenerative Medicine
Abstract

The purpose of this study was to investigate the adhesion properties and tissue reactions in an in vitro model of nanofabricated membranes emulating the vitreous cortex. Electrospinning was performed for either 5, 10 or 15 min to create various thicknesses of poly(ε-caprolactone) (PCL) fibre mats on a poly(glycerol-co-sebacic acid) (PGS) surface. These were fused with adult porcine retinal explants, with the fibre side facing the inner retina, and cultured for 5 days. Adherence was assessed by macroscopic inspection, and morphological and immunohistochemical analysis was performed using haematoxylin and eosin (H&E) and markers for photoreceptors and Müller glia (recoverin, NeuN, vimentin and GFAP). TUNEL labelling was performed to... (More)

The purpose of this study was to investigate the adhesion properties and tissue reactions in an in vitro model of nanofabricated membranes emulating the vitreous cortex. Electrospinning was performed for either 5, 10 or 15 min to create various thicknesses of poly(ε-caprolactone) (PCL) fibre mats on a poly(glycerol-co-sebacic acid) (PGS) surface. These were fused with adult porcine retinal explants, with the fibre side facing the inner retina, and cultured for 5 days. Adherence was assessed by macroscopic inspection, and morphological and immunohistochemical analysis was performed using haematoxylin and eosin (H&E) and markers for photoreceptors and Müller glia (recoverin, NeuN, vimentin and GFAP). TUNEL labelling was performed to assess apoptosis. Five minute specimens displayed poor adherence with an overall structure, apoptosis and photoreceptor and ganglion cell morphology comparable to that of the culture controls, whereas 10 min specimens showed improved neuronal survival; 15 min composite explants adhered only at focal points, were thin and showed extensive degenerative damage. The physical composition of nanofibre meshes is important for adhesion to the inner retina and has a significant impact on neuronal and glial survival in vitro. The results bearing on research involving retinal transplantation are discussed.

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author
organization
publishing date
type
Contribution to journal
publication status
in press
subject
keywords
Biomechanics, Neuronal survival, PCL, PGS, Retina, Retinal cultures, Tissue stabilization
in
Journal of Tissue Engineering and Regenerative Medicine
publisher
John Wiley & Sons
external identifiers
  • Scopus:84964339414
ISSN
1932-6254
DOI
10.1002/term.2135
language
English
LU publication?
yes
id
f87cd2f3-44cd-4139-bb8f-20955128ddd2
date added to LUP
2016-06-01 12:48:52
date last changed
2016-11-08 15:05:33
@misc{f87cd2f3-44cd-4139-bb8f-20955128ddd2,
  abstract     = {<p>The purpose of this study was to investigate the adhesion properties and tissue reactions in an in vitro model of nanofabricated membranes emulating the vitreous cortex. Electrospinning was performed for either 5, 10 or 15 min to create various thicknesses of poly(ε-caprolactone) (PCL) fibre mats on a poly(glycerol-co-sebacic acid) (PGS) surface. These were fused with adult porcine retinal explants, with the fibre side facing the inner retina, and cultured for 5 days. Adherence was assessed by macroscopic inspection, and morphological and immunohistochemical analysis was performed using haematoxylin and eosin (H&amp;E) and markers for photoreceptors and Müller glia (recoverin, NeuN, vimentin and GFAP). TUNEL labelling was performed to assess apoptosis. Five minute specimens displayed poor adherence with an overall structure, apoptosis and photoreceptor and ganglion cell morphology comparable to that of the culture controls, whereas 10 min specimens showed improved neuronal survival; 15 min composite explants adhered only at focal points, were thin and showed extensive degenerative damage. The physical composition of nanofibre meshes is important for adhesion to the inner retina and has a significant impact on neuronal and glial survival in vitro. The results bearing on research involving retinal transplantation are discussed.</p>},
  author       = {Taylor, Linnéa and Arnér, Karin and Kolewe, Martin and Pritchard, Christopher and Hendy, Gillian and Langer, Robert and Ghosh, Fredrik},
  issn         = {1932-6254},
  keyword      = {Biomechanics,Neuronal survival,PCL,PGS,Retina,Retinal cultures,Tissue stabilization},
  language     = {eng},
  publisher    = {ARRAY(0xb485138)},
  series       = {Journal of Tissue Engineering and Regenerative Medicine},
  title        = {Seeing through the interface : Poly(ε-Caprolactone) surface modification of poly(glycerol-co-sebacic acid) membranes in adult porcine retinal explants},
  url          = {http://dx.doi.org/10.1002/term.2135},
  year         = {2016},
}