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Analysis by high-performance liquid chromatography of radioactively labeled carbohydrate components of proteoglycans

Lohmander, Stefan LU (1986) In Analytical Biochemistry 154(1). p.75-84
Abstract

Methods were developed for the separation of radioactively labeled carbohydrate components of proteoglycans by isocratic ion-moderated partition HPLC. Neutral sugars were separated after hydrolysis in trifluoroacetic acid with baseline separation between glucose, xylose, galactose, fucose, and mannose. N-Acetylneuraminic acid, N-acetylated hexosamines, glucose, galactose, and xylitol were likewise well separated from each other under isocratic elution conditions. Glucuronic acid, iduronic acid, and their lactones were separated after hydrolysis in formic acid and sulfuric acid. Glucosamine, galactosamine, galactosaminitol, and glucosaminitol were separated by HPLC on a cation exchanger with neutral buffer after hydrolysis in... (More)

Methods were developed for the separation of radioactively labeled carbohydrate components of proteoglycans by isocratic ion-moderated partition HPLC. Neutral sugars were separated after hydrolysis in trifluoroacetic acid with baseline separation between glucose, xylose, galactose, fucose, and mannose. N-Acetylneuraminic acid, N-acetylated hexosamines, glucose, galactose, and xylitol were likewise well separated from each other under isocratic elution conditions. Glucuronic acid, iduronic acid, and their lactones were separated after hydrolysis in formic acid and sulfuric acid. Glucosamine, galactosamine, galactosaminitol, and glucosaminitol were separated by HPLC on a cation exchanger with neutral buffer after hydrolysis in hydrochloric acid. The separation techniques also proved useful in fractionation of exoglycosidase digests of O- and N-linked oligosaccharides. Separations of aldoses, hexosamines, and uronic acids were adapted to sensitive photometric detection. © 1986.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
glycoproteins, HPLC carbohydrates, liquid scintillation counting, polysaccharides, protein processing, sugar analysis
in
Analytical Biochemistry
volume
154
issue
1
pages
10 pages
publisher
Elsevier
external identifiers
  • Scopus:0022445512
ISSN
0003-2697
DOI
10.1016/0003-2697(86)90498-7
language
English
LU publication?
yes
id
fc90eeeb-55b3-4487-93ce-ac0e74c492bc
date added to LUP
2016-05-04 12:59:00
date last changed
2016-11-20 04:33:11
@misc{fc90eeeb-55b3-4487-93ce-ac0e74c492bc,
  abstract     = {<p>Methods were developed for the separation of radioactively labeled carbohydrate components of proteoglycans by isocratic ion-moderated partition HPLC. Neutral sugars were separated after hydrolysis in trifluoroacetic acid with baseline separation between glucose, xylose, galactose, fucose, and mannose. N-Acetylneuraminic acid, N-acetylated hexosamines, glucose, galactose, and xylitol were likewise well separated from each other under isocratic elution conditions. Glucuronic acid, iduronic acid, and their lactones were separated after hydrolysis in formic acid and sulfuric acid. Glucosamine, galactosamine, galactosaminitol, and glucosaminitol were separated by HPLC on a cation exchanger with neutral buffer after hydrolysis in hydrochloric acid. The separation techniques also proved useful in fractionation of exoglycosidase digests of O- and N-linked oligosaccharides. Separations of aldoses, hexosamines, and uronic acids were adapted to sensitive photometric detection. © 1986.</p>},
  author       = {Lohmander, Stefan},
  issn         = {0003-2697},
  keyword      = {glycoproteins,HPLC carbohydrates,liquid scintillation counting,polysaccharides,protein processing,sugar analysis},
  language     = {eng},
  number       = {1},
  pages        = {75--84},
  publisher    = {ARRAY(0xa073458)},
  series       = {Analytical Biochemistry},
  title        = {Analysis by high-performance liquid chromatography of radioactively labeled carbohydrate components of proteoglycans},
  url          = {http://dx.doi.org/10.1016/0003-2697(86)90498-7},
  volume       = {154},
  year         = {1986},
}