1.1 191 info:srw/schema/1/mods-v3.3xml journalArticle published yes 10 D. L. A. Van den Hove author G. Kenis author A. Brass author R. Opstelten author B. P. F. Rutten author Matteo Bruschettini author 49c78950-b068-4fa9-808c-1f91efc3b24b C. E. Blanco author K. P. Lesch author H. W. M. Steinbusch author J. Prickaerts author Adverse life events during pregnancy may impact upon the developing fetus, predisposing prenatally stressed offspring to the development of psychopathology. In the present study, we examined the effects of prenatal restraint stress (PS) on anxiety- and depression-related behavior in both male and female adult Sprague-Dawley rats. In addition, gene expression profiles within the hippocampus and frontal cortex (FC) were examined in order to gain more insight into the molecular mechanisms that mediate the behavioral effects of PS exposure. PS significantly increased anxiety-related behavior in male, but not female offspring. Likewise, depression-related behavior was increased in male PS rats only. Further, male PS offspring showed increased basal plasma corticosterone levels in adulthood, whereas both PS males and females had lower stress-induced corticosterone levels when compared to controls. Microarray-based profiling of the hippocampus and FC showed distinct sex-dependent changes in gene expression after PS. Biological processes and/or signal transduction cascades affected by PS included glutamatergic and GABAergic neurotransmission, mitogen-activated protein kinase (MAPK) signaling, neurotrophic factor signaling, phosphodiesterase (PDE)/ cyclic nucleotide signaling, glycogen synthase kinase 3 (GSK3) signaling, and insulin signaling. Further, the data indicated that epigenetic regulation is affected differentially in male and female PS offspring. These sex-specific alterations may, at least in part, explain the behavioral differences observed between both sexes, i.e. relative vulnerability versus resilience to PS in male versus female rats, respectively. These data reveal novel potential targets for antidepressant and mood stabilizing drug treatments including PDE inhibitors and histone deacetylase (HDAC) inhibitors. (C) 2012 Elsevier B.V. and ECNP. All rights reserved. https://portal.research.lu.se/en/publications/52c0cb16-8f04-4920-92b1-9af01d17d158 Elsevier 2013 eng Prenatal stress Depression Anxiety Microarray Resilience Epigenetics Neurosciences 1873-7862 7856168 000325833500010 84884289212 23199416 10.1016/j.euroneuro.2012.09.011 http://dx.doi.org/10.1016/j.euroneuro.2012.09.011 23 10 1226 1246 https://www.scopus.com/pages/publications/84884289212 yes 52c0cb16-8f04-4920-92b1-9af01d17d158 2016-04-01T10:31:24+02:00 2025-10-14T13:10:58+02:00 2016-04-01T10:31:24+02:00 1 info:srw/schema/1/mods-v3.3xml journalArticle published yes 11 Fabrizio Michetti author Matteo Bruschettini author 49c78950-b068-4fa9-808c-1f91efc3b24b Alessandro Frigiola author Raul Abella author Alessandro Giamberti author Nora Marchese author Salvatore Mangraviti author Giovanni Melioli author Amedeo Baldari author Pierluigi Bruschettini author Diego Gazzolo author Background: Neurological dysfunction is a key medical concern in professional sportsmen (PSM). We investigated whether saliva S100B concentrations in PSM and healthy controls are modified before and after training. Methods: We conducted a case-control-study in 75 patients (25 PSM vs 50 controls) in which S100B saliva concentrations were expressed as absolute values and percentage of change (%) from samples drawn before (T0) and after (T1) training. Results: No differences (P>0.05) between groups were found regarding clinical, monitoring and laboratory parameters. S100B both in PSM and controls was higher at T1 when compared to TO (P<0.01). In PSM, S100B was higher than controls (P<0.001) at TO and T1. S100B% at T0-T1 was higher (P<0.001) in PSM and in controls and between PSM and controls (P<0.001). Conclusions: Increased saliva S100B levels in PSM before and after training suggest a paracrine/autocrine protein's role connected to stressing activity, which becomes especially evident in PSMs. (C) 2010 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved. https://portal.research.lu.se/en/publications/5a294f45-2647-4600-9fc1-df3bb44936cc Elsevier 2011 eng Neurodegenerative disorders Saliva S100B protein Sport Medicinal Chemistry Neurology 1873-2933 7855486 000286959100018 79151480512 20970414 10.1016/j.clinbiochem.2010.10.007 http://dx.doi.org/10.1016/j.clinbiochem.2010.10.007 yes 44 2-3 245 247 https://www.scopus.com/pages/publications/79151480512 yes 5a294f45-2647-4600-9fc1-df3bb44936cc 2016-04-01T10:50:30+02:00 2025-10-14T11:34:56+02:00 2016-04-01T10:50:30+02:00 2 info:srw/schema/1/mods-v3.3xml journalArticle published yes 8 Sebastiano Barco author Francesco Maria Risso author Matteo Bruschettini author 49c78950-b068-4fa9-808c-1f91efc3b24b Roberto Bandettini author Luca A. Ramenghi author Gino Tripodi author Elio Castagnola author Giuliana Cangemi author Background Therapeutic drug monitoring (TDM) of antibiotics in children is of fundamental importance for effective patient management. The use of dried blood spot (DBS) offers a number of advantages over conventional blood collection. The aim of this study is to develop and validate a method to measure piperacillin/tazobactam in DBS. Results The analysis was performed by using LC-MS/MS operating in multiple reaction monitoring mode. The method has been validated by applying EMA guidelines and its suitability for TDM was evaluated by using samples from low birth weight neonates. Conclusion This paper describes a fast and cost-effective micromethod for the simultaneous determination of piperacillin/tazobactam levels on dried blood spot that is suitable for TDM in children. https://portal.research.lu.se/en/publications/0e4f2040-3a65-410d-b4bb-95ce30a7e813 Taylor & Francis 2014 eng Pediatrics 1757-6180 7855465 000346699600002 84918496205 10.4155/BIO.14.205 http://dx.doi.org/10.4155/BIO.14.205 yes 6 21 2795 2802 https://www.scopus.com/pages/publications/84918496205 yes 0e4f2040-3a65-410d-b4bb-95ce30a7e813 2016-04-01T10:53:01+02:00 2025-10-14T12:58:22+02:00 2016-04-01T10:53:01+02:00 3 info:srw/schema/1/mods-v3.3xml journalArticle published yes 9 Giuliana Cangemi author Simona Storti author Massimiliano Cantinotti author Antonio Fortunato author Michele Emdin author Matteo Bruschettini author 49c78950-b068-4fa9-808c-1f91efc3b24b Daniela Bugnone author Giovanni Melioli author Aldo Clerico author Background: Neutrophil gelatinase-associated lipocalin (NGAL) has been suggested as the most promising biomarker of acute kidney injury. However, there are no reliable data on analytical sensitivity and reference limits of urinary NGAL (uNGAL) assay in pediatric age. The aim of the present study is to evaluate the analytical sensitivity and the reference range of uNGAL measured in urine specimens of pediatric age with the fully automated platform ARCHITECT (R) i1000. Methods: A total of 333 urine samples were collected from 25 healthy newborns (16 males and 9 females; age 1-4 days) and 308 children (150 males and 147 females; mean age 80.7 months, range 0.63-248 months) and assayed for uNGAL by two different Italian centers (Department of Laboratory Medicine of the Fondazione Toscana G. Monasterio of Pisa and Massa and the Clinical Pathology Laboratory Unit of Istituto Giannina Gaslini of Genova). Results: The calculated limits of blank (LOB) and detection (LOD) values were 0.5 ng/mL and 0.95 ng/mL, respectively. The distribution of uNGAL values approximated a log-normal distribution (median 5.2 ng/mL, interquartile range 2.5-12.8 ng/mL, 99th percentile 117.6 ng/mL). uNGAL values of the 25 neonates were significantly higher than those of 308 children (neonates: mean 44.2 ng/mL, median 30.3 ng/mL, range 5.2-137.4 ng/mL; children: mean 10.2 ng/mL, median 4.6 ng/mL, range 0.2-146.7 ng/mL; p<0.0001). Conclusions: uNGAL assay shows a good analytical sensitivity and imprecision, which allows the measurement of uNGAL values around the cut-off value (i.e., 117.6 ng/mL) with an imprecision <5 CV%. The distribution of uNGAL values in pediatric age approximates a log-normal distribution, with values which are higher in neonates compared to children. https://portal.research.lu.se/en/publications/8d77ed5f-885a-4e61-afec-4dc466ff8147 De Gruyter 2013 eng reference values urine pediatric age NGAL creatinine Medicinal Chemistry Pediatrics 1434-6621 7855475 000318495900033 84882263723 23183760 10.1515/cclm-2012-0540 http://dx.doi.org/10.1515/cclm-2012-0540 yes 51 5 1101 1105 https://www.scopus.com/pages/publications/84882263723 yes 8d77ed5f-885a-4e61-afec-4dc466ff8147 2016-04-01T11:06:40+02:00 2025-10-14T08:59:12+02:00 2016-04-01T11:06:40+02:00 4 info:srw/schema/1/mods-v3.3xml journalArticle published yes 8 D Gazzolo author F Michetti author Matteo Bruschettini author 49c78950-b068-4fa9-808c-1f91efc3b24b N Marchese author M Lituania author S Mangraviti author E Pedrazzi author P Bruschettini author https://portal.research.lu.se/en/publications/592a91b5-ba91-403b-bd9f-4bd0271c8b46 Oxford University Press 2003 eng Pediatrics 0009-9147 7856403 000183088100023 0037683347 10.1373/49.6.967 http://dx.doi.org/10.1373/49.6.967 yes 49 6 967 970 https://www.scopus.com/pages/publications/0037683347 yes 592a91b5-ba91-403b-bd9f-4bd0271c8b46 2016-04-01T11:37:12+02:00 2025-10-14T09:48:56+02:00 2016-04-01T11:37:12+02:00 5 info:srw/schema/1/mods-v3.3xml journalArticle published yes 11 D Gazzolo author E Marinoni author R Di Iorio author M Lituania author M Marras author Matteo Bruschettini author 49c78950-b068-4fa9-808c-1f91efc3b24b P Bruschettini author R Frulio author F Michetti author F Petraglia author P Florio author Background: Intrauterine growth restriction (IUGR) is associated with perinatal mortality and with neurologic damage from intraventricular hemorrhage (IVH). We investigated whether S100B, a neural protein found in high concentrations after cell injury in the nervous system, is increased in serum of women whose pregnancies are complicated by IUGR and whose newborns develop IVH. We also explored the prognostic accuracy of maternal serum S100B for IVH in the newborn. Methods: We conducted a case-control study of 106 pregnancies complicated by IUGR, including a subgroup (n = 26) who developed IVH after birth, and 212 unaffected pregnancies matched for gestational age. Ultrasound examination, Doppler velocimetry patterns (in the utero-placental vessels and middle cerebral artery), and maternal blood collection were performed before birth; cerebral ultrasound and neurologic examinations were performed after birth. Results: S100B was higher (P < 0.001) in IUGR pregnancies complicated by IVH than in those that were not and in controls. At a cutoff of 0.72 mu g/L, sensitivity was 100% [95% confidence interval (95% CI), 87%-100%] and specificity was 99.3% (97.5%-99.9%) for prediction of IVH (area under the ROC curve, 0.999). The prevalence of IVH was 8.2% in the whole study population, 93% (95% CI, 83.6%-100%) in those with maternal S100B > 0.72 mu g/L, and 0% (0%-2.5%) in those with maternal S100B < 0.72 mu g/L. Conclusion: For prediction of IVH, measurements of maternal S100B may be useful at times before clinical, laboratory, and ultrasound patterns can identify risk of IVH. (c) 2006 American Association for Clinical Chemistry. https://portal.research.lu.se/en/publications/4c99cd3c-ffac-41a0-9c0d-4e17744a722b Oxford University Press 2006 eng Gynaecology, Obstetrics and Reproductive Medicine 0009-9147 7856209 000237339600006 33646370239 10.1373/clinchem.2005.060665 http://dx.doi.org/10.1373/clinchem.2005.060665 yes 52 5 819 826 https://www.scopus.com/pages/publications/33646370239 yes 4c99cd3c-ffac-41a0-9c0d-4e17744a722b 2016-04-01T11:41:08+02:00 2025-10-14T11:30:58+02:00 2016-04-01T11:41:08+02:00 6 info:srw/schema/1/mods-v3.3xml journalArticle published yes 8 DLA Van den Hove author CE Blanco author B Aendekerk author L Desbonnet author Matteo Bruschettini author 49c78950-b068-4fa9-808c-1f91efc3b24b HP Steinbusch author J Prickaerts author HWM Steinbusch author Chronic or repeated stress during critical periods of human fetal brain development has been associated with various learning, behavioral and/or mood disorders in later life. In this investigation, pregnant Fischer 344 rats was individually restrained three times a day for 45 min during the last week of gestation in transparent plastic cylinders while at the same time being exposed to bright light. Control pregnant females were left undisturbed in their home cages. Anxiety and depressive-like behavior was measured in the offspring at an age of 6 months using the open field test, the home cage emergence test and the forced swim test. Prenatally stressed rats spent more time in the corners and less time along the walls of an open field, while no difference in total distance moved was observed. In addition, prenatally stressed rats took more time to leave their home cage in the home cage emergence test. On the other hand, no differences in immobility were observed in the forced swim test. Moreover, prenatally stressed rats showed lower stress-induced plasma corticosterone levels compared with control rats. Prenatal stress (PS) had no effect on the number of 5-bromo-2-deoxyuridine-positive cells - used as a measure for cell proliferation - in the dentate gyrus of these rats. These data further support the idea that PS may perturb normal anxiety-related development. However, the present data also suggest that an adaptive or protective effect of PS should not be ignored. Genetic factors are likely to play a role in this respect. Copyright (c) 2005 S. Karger AG, Basel. https://portal.research.lu.se/en/publications/adfbd0b6-e11e-4eda-8001-1cb7fdcb3940 Karger 2005 eng depression anxiety neurogenesis hypothalamo-pituitary-adrenal axis pregnancy Neurology 1421-9859 7856300 000231701800005 24144438221 10.1159/000086711 http://dx.doi.org/10.1159/000086711 yes 27 5 313 320 https://www.scopus.com/pages/publications/24144438221 yes adfbd0b6-e11e-4eda-8001-1cb7fdcb3940 2016-04-01T11:41:42+02:00 2025-10-14T10:08:20+02:00 2016-04-01T11:41:42+02:00 7 info:srw/schema/1/mods-v3.3xml journalArticle published yes 6 D Gazzolo author Matteo Bruschettini author 49c78950-b068-4fa9-808c-1f91efc3b24b M Lituania author G Serra author E Gandullia author F Michetti author https://portal.research.lu.se/en/publications/76a07dfb-ca0d-4f42-9314-c8c2db0325c4 Oxford University Press 2001 eng Pediatrics 0009-9147 7856455 000168996300032 0035013087 47 6 1132 1133 https://www.scopus.com/pages/publications/0035013087 yes 76a07dfb-ca0d-4f42-9314-c8c2db0325c4 2016-04-01T11:41:45+02:00 2025-10-14T13:24:30+02:00 2016-04-01T11:41:45+02:00 8 info:srw/schema/1/mods-v3.3xml journalArticle published yes 8 D Gazzolo author D Grutzfeld author F Michetti author A Toesca author M Lituania author Matteo Bruschettini author 49c78950-b068-4fa9-808c-1f91efc3b24b A Dobrzanska author PL Bruschettini author https://portal.research.lu.se/en/publications/b23adc7b-2c45-43c9-9ea1-2b21bff89397 Oxford University Press 2004 eng Pediatrics 0009-9147 7856342 000221118800024 2142651546 10.1373/clinchem.2003.021048 http://dx.doi.org/10.1373/clinchem.2003.021048 yes 50 5 941 944 https://www.scopus.com/pages/publications/2142651546 yes b23adc7b-2c45-43c9-9ea1-2b21bff89397 2016-04-01T11:42:19+02:00 2025-10-14T10:12:40+02:00 2016-04-01T11:42:19+02:00 9 info:srw/schema/1/mods-v3.3xml journalArticle published yes 8 P Florio author F Michetti author Matteo Bruschettini author 49c78950-b068-4fa9-808c-1f91efc3b24b M Lituania author P Bruschettini author FM Severi author F Petraglia author D Gazzolo author Fetal death in the mid-trimester of pregnancy is unexplained and no reliable markers are available to identify at-risk women. We aimed to assess use of a fetoprotein and S100B concentrations in amniotic fluid as markers. We did a case-control study in 758 healthy women undergoing amniocentesis at mid-gestation, of whom 12 had a spontaneous intrauterine fetal death before 28 weeks, and 746 matched controls. Concentrations were corrected for gestational age by conversion to multiples of median (MoM) of healthy controls of the same gestational age. Concentrations of S100B, but not alpha fetoprotein, were significantly higher (p<0.0001) in women who later had spontaneous fetal death (median 4.431 MoM [95%CI 3.605-6.197]) than in controls (1.000 MoM [1.062-1.121]). Sensitivity, specificity, and positive and negative predictive values of S100B as a diagnostic test were 100%, suggesting that measurement of this protein at amniocentesis could be useful to identify at-risk women. https://portal.research.lu.se/en/publications/31dad38e-e504-4cce-b611-98e8298ee6cc Elsevier 2004 eng Gynaecology, Obstetrics and Reproductive Medicine 1474-547X 7856332 000222784900030 3242685259 10.1016/S0140-6736(04)16677-4 http://dx.doi.org/10.1016/S0140-6736(04)16677-4 yes 364 9430 270 272 https://www.scopus.com/pages/publications/3242685259 yes 31dad38e-e504-4cce-b611-98e8298ee6cc 2016-04-01T11:45:22+02:00 2025-10-14T13:15:03+02:00 2016-04-01T11:45:22+02:00 10 info:srw/schema/1/mods-v3.3xml conference abstract published yes 5 DSM Gazzolo author Matteo Bruschettini author 49c78950-b068-4fa9-808c-1f91efc3b24b G Buonocore author G Serra author F Michetti author Annual Meeting of the Pediatric-Academic-Societies https://portal.research.lu.se/en/publications/51a46dea-ab60-46f8-adc7-9870ff68d863 International Pediatric Foundation Inc. 2004 2004-05-04 eng Pediatrics 1530-0447 7856352 000220591102414 55 4 413 413 yes 51a46dea-ab60-46f8-adc7-9870ff68d863 2016-04-01T11:46:55+02:00 2025-04-04T14:25:16+02:00 2016-04-01T11:46:55+02:00 11 info:srw/schema/1/mods-v3.3xml journalArticle published yes 10 D Gazzolo author P Florio author S Ciotti author E Marinoni author R Di Iorio author Matteo Bruschettini author 49c78950-b068-4fa9-808c-1f91efc3b24b R Sacchi author G Serra author M Lituania author F Michetti author Prematurity is an important cause of perinatal death, and no reliable biochemical/biophysical markers exist to identify newborns with an increased mortality risk. We aimed to use S100B concentrations in urine as an early indicator of risk of neonatal death. We did a cross-sectional study using urine obtained from 165 preterm newborns, of whom I I suffered neonatal death within the first week, 121 displayed no overt neurologic syndrome, and 33 suffered neonatal hypoxia and intraventricular hemorrhage (IVH) but not ominous outcome. Urine S100B concentrations were determined at four time-points and corrected for gestational age by conversion to multiples of median (MoM) of healthy controls of the same gestational age. Ultrasound imaging was assessed within the first 72 h from birth. In infants that died within the first week, S100B levels in urine were already higher than controls at first urination and increased progressively between the 24 and 96-h time-points. Multiple logistic regression analysis showed a significant correlation between urine S100B protein concentrations and the occurrence of neonatal death. An S100B concentration cut-off of 12.93 MoM at first urination had a sensitivity of 100% and a specificity of 97.8% for predicting an ominous outcome. The positive predictive value was 78.6%, the negative predictive value was 100%. Measurement of urine S100B protein levels in preterm newborns could be useful to identify newborns at higher risk of neonatal death. https://portal.research.lu.se/en/publications/0f2175f0-cab3-4cd4-9eef-1efdc4de28e4 International Pediatric Foundation Inc. 2005 eng Pediatrics 1530-0447 7856234 000233416500006 33644801670 10.1203/01.pdr.0000185131.22985.30 http://dx.doi.org/10.1203/01.pdr.0000185131.22985.30 yes 58 6 1170 1174 https://www.scopus.com/pages/publications/33644801670 yes 0f2175f0-cab3-4cd4-9eef-1efdc4de28e4 2016-04-01T11:52:35+02:00 2025-10-14T09:09:43+02:00 2016-04-01T11:52:35+02:00 12 info:srw/schema/1/mods-v3.3xml journalArticle published yes 9 Matteo Bruschettini author 49c78950-b068-4fa9-808c-1f91efc3b24b DLA Van den Hove author S Timmers author M Welling author HP Steinbusch author J Prickaerts author D Gazzolo author CE Blanco author HWM Steinbusch author We investigated the effects of a single course of antenatal betamethasone on cognition- and anxiety-related behavior and synaptophysin and microtubule-associated protein 2 (MAP2) immunoreactivity in the adult rat hippocampus. On d 20 of gestation, pregnant rats were injected with either 1) 170 mu g/kg body weight of betamethasone ("clinically equivalent dose," equivalent to 12 mg twice, 24 h apart); 2) half this dose; or 3) vehicle. Cognition- and anxiety-related behavior of the offspring was analyzed at an age of 5 mo using the Morris water maze, object recognition task, and open field test. Subsequently, synaptophysin and MAP2 immunoreactivity were measured in the hippocampus. We report no detrimental effects of antenatal betamethasone on cognition- and anxiety-related behavior and synaptophysin immunoreactivity in the adult rat. On the other hand, MAP2 immunoreactivity was decreased by betamethasone in males, suggesting a permanent impairment in the hippocampus. Interestingly, the lower dose appears to have less influence in terms of growth restriction, known to be associated with an increased risk of disease in adulthood. Further research might elucidate whether the betamethasone effect on hippocampal neurons persists later in life and could affect the aging process increasing the risk for neuropathology of the adult. https://portal.research.lu.se/en/publications/19d6e6cf-3c86-40ff-a6c1-ec4571fd1559 International Pediatric Foundation Inc. 2006 eng Pediatrics 1530-0447 7856191 000238456800010 33748086489 10.1203/01.pdr.0000220349.41675.92 http://dx.doi.org/10.1203/01.pdr.0000220349.41675.92 yes 60 1 50 54 https://www.scopus.com/pages/publications/33748086489 yes 19d6e6cf-3c86-40ff-a6c1-ec4571fd1559 2016-04-01T11:57:16+02:00 2025-10-14T11:34:46+02:00 2016-04-01T11:57:16+02:00 13 info:srw/schema/1/mods-v3.3xml journalArticle published yes 7 D Gazzolo author Matteo Bruschettini author 49c78950-b068-4fa9-808c-1f91efc3b24b V Corvino author M Lituania author R Sarli author P Bruschettini author F Michetti author Background: The human chromosome 21 has been shown to contain the gene for the subunit of the S100B protein. The present case-control study was aimed at investigating whether overproduction of S100B protein is detectable in the amniotic fluid of foetuses with trisomy-21. Methods: Measurements of S 10013 in amniotic fluid samples from 14 pregnant women with trisomy-21 foetuses were compared with those obtained from 182 physiological pregnancies. S100B was measured in the samples using an immunoluminometric assay (LIA-mat Sangtec 100). Results: Our results showed that S100B protein amniotic fluid levels were significantly higher in trisomy-21 foetuses (0.83 +/- 0.21 mug/l) than in controls (0.51 +/- 0.22 mug/l) (p < 0.05). Conclusion: The present finding supports the notion that the expression of S100B is increased in trisomy-21 foetuses; it also constitutes a prerequisite basis for a possible involvement of the protein in pathogenic processes associated with trisomy-21, and/or for its potential employment as a diagnostic tool. (C) 2003 Elsevier Science B.V. All rights reserved. https://portal.research.lu.se/en/publications/04134fda-9211-4356-979a-a7b3014bfa66 Elsevier 2003 eng trisomy-21 ammotic fluid brain maturation foetus S100B protein Gynaecology, Obstetrics and Reproductive Medicine 0009-8981 7856420 000181953800008 0037375570 10.1016/S0009-8981(03)00010-X http://dx.doi.org/10.1016/S0009-8981(03)00010-X yes 330 1-2 131 133 https://www.scopus.com/pages/publications/0037375570 yes 04134fda-9211-4356-979a-a7b3014bfa66 2016-04-01T11:59:40+02:00 2025-10-14T09:15:51+02:00 2016-04-01T11:59:40+02:00 14 info:srw/schema/1/mods-v3.3xml journalArticle published yes 6 D Gazzolo author Matteo Bruschettini author 49c78950-b068-4fa9-808c-1f91efc3b24b M Lituania author G Serra author W Bonacci author F Michetti author https://portal.research.lu.se/en/publications/109fad93-3c80-4a9f-9fec-f3a65ec7d668 Oxford University Press 2001 eng Pediatrics 0009-9147 7856448 000171098600014 0034741555 47 10 1836 1838 https://www.scopus.com/pages/publications/0034741555 yes 109fad93-3c80-4a9f-9fec-f3a65ec7d668 2016-04-01T12:00:46+02:00 2025-10-14T10:56:37+02:00 2016-04-01T12:00:46+02:00 15 info:srw/schema/1/mods-v3.3xml journalArticle published yes 9 D Gazzolo author Matteo Bruschettini author 49c78950-b068-4fa9-808c-1f91efc3b24b R Di Iorio author E Marinoni author M Lituania author M Marras author R Sarli author PL Bruschettini author F Michetti author https://portal.research.lu.se/en/publications/925658b1-ac7a-41cf-8470-1999aa955970 Oxford University Press 2002 eng Gynaecology, Obstetrics and Reproductive Medicine 0009-9147 7856438 000174535300009 0036201285 48 4 647 650 https://www.scopus.com/pages/publications/0036201285 yes 925658b1-ac7a-41cf-8470-1999aa955970 2016-04-01T12:02:51+02:00 2025-10-14T09:43:01+02:00 2016-04-01T12:02:51+02:00 16 info:srw/schema/1/mods-v3.3xml journalArticle published yes 10 D Gazzolo author M Lituania author Matteo Bruschettini author 49c78950-b068-4fa9-808c-1f91efc3b24b S Ciotti author R Sacchi author G Serra author MG Calevo author V Corvino author G Buonocore author F Michetti author Objectives: S100B is an acidic calcium-binding protein of the EF-hand family present in the central nervous system, where it is concentrated in glial cells. It has been suggested to act as a cytokine with neurotrophic effects at physiological concentrations. Design and methods: S100B concentration was assessed in saliva by western blot analysis and an immunoluminometric assay. A reference curve of the protein was established in 216 preterm and term newborns. Results: S100B levels were significantly higher in saliva taken from the preterm group, and the highest S100B levels were found in newborns who were delivered in the earlier weeks of gestation, exhibiting a progressive decrease nearer to term. S100B concentration in saliva was correlated with gestational age (r = -0.69; P < 0.001). Conclusions: The present study offers data consistent with the putative neurotrophic role of S100B and suggests the usefulness of saliva in the clinical monitoring of S100B levels. (C) 2005 The Canadian Society of Clinical Chemists. All rights reserved. https://portal.research.lu.se/en/publications/90674a4e-c358-4a54-afb2-af1bd0d0c549 Elsevier 2005 eng S100B protein newborn saliva cytokine neurotrophic factor central nervous system Pediatrics 1873-2933 7856278 000227206700004 20844431858 10.1016/j.clinbiochem.2004.12.006 http://dx.doi.org/10.1016/j.clinbiochem.2004.12.006 yes 38 3 229 233 https://www.scopus.com/pages/publications/20844431858 yes 90674a4e-c358-4a54-afb2-af1bd0d0c549 2016-04-01T12:07:25+02:00 2025-10-14T11:34:29+02:00 2016-04-01T12:07:25+02:00 17 info:srw/schema/1/mods-v3.3xml journalArticle published yes 8 Daniel L. A. Van den Hove author Harry W. M. Steinbusch author Matteo Bruschettini author 49c78950-b068-4fa9-808c-1f91efc3b24b Diego Gazzolo author Rosanna Frulio author Arjan Scheepens author Jos Prickaerts author Carlos E. Blanco author Prenatal stress has been shown to disturb neonatal rat brain development. The astroglial-specific neurotrophic factor S100B is known to play an important role in normal brain development. In the present study, we investigated the effects of prenatal stress on S100B concentrations in the hippocampus of I-day-old Fischer 344 rats. Overall, prenatal stress resulted in a 25% reduction in hippocampal S100B content. Further, male hippocampal S100B content was negatively correlated with plasma corticosterone levels. Positive correlations were found between female S100B levels and fetal growth, and hippocampal brain-derived neurotrophic factor content. In conclusion, the observed reduction in neonatal hippocampal S100B levels, as a consequence of prenatal stress, may be involved in affecting postnatal brain development. https://portal.research.lu.se/en/publications/1195a05d-9419-404d-b150-983a19b22678 Lippincott Williams & Wilkins 2006 eng anxiety depression development pregnancy serotonin Neurology 1473-558X 7855513 000239213000026 33745475069 10.1097/01.wnr.0000223391.74575.c9 http://dx.doi.org/10.1097/01.wnr.0000223391.74575.c9 yes 17 10 1077 1080 https://www.scopus.com/pages/publications/33745475069 yes 1195a05d-9419-404d-b150-983a19b22678 2016-04-01T12:14:47+02:00 2025-10-14T13:26:50+02:00 2016-04-01T12:14:47+02:00 18 info:srw/schema/1/mods-v3.3xml journalArticle published yes 8 D Gazzolo author M Kornacka author Matteo Bruschettini author 49c78950-b068-4fa9-808c-1f91efc3b24b M Lituania author L Giovannini author G Serra author U Majewska author F Michetti author https://portal.research.lu.se/en/publications/c1cbc030-6037-421e-b6c7-ddb60dead235 Oxford University Press 2003 eng Pediatrics 0009-9147 7856393 000183789600035 0038610850 10.1373/49.7.1215 http://dx.doi.org/10.1373/49.7.1215 yes 49 7 1215 1218 https://www.scopus.com/pages/publications/0038610850 yes c1cbc030-6037-421e-b6c7-ddb60dead235 2016-04-01T12:21:41+02:00 2025-10-14T12:52:49+02:00 2016-04-01T12:21:41+02:00 19 info:srw/schema/1/mods-v3.3xml journalArticle published yes 5 Matteo Bruschettini author 49c78950-b068-4fa9-808c-1f91efc3b24b DLA van den Hove author D Gazzolo author HWM Steinbusch author CE Blanco author We investigated the effects of a single course of antenatal betamethasone on neonatal somatic and brain development. On day 20 of gestation, pregnant rats were injected with either with 170 mu g kg(-1) body weight of betamethasone ("clinically-equivalent dose," equivalent to 12 mg twice, 24 hours apart) or half this dose or vehicle. Pups (8-11 animals per experimental group per time-point per gender) were analyzed at 1 (P1), 2, and 21 days after birth. We report that betamethasone induced a significant dose-dependent decrease of somatic measurements in both genders. At PI cell proliferation was affected by the "clinically equivalent dose" only in the subventricular zone in both genders and in the hippocampus in males. In summary, we show for the first time that a lower dose (equivalent to 6 mg) induces fewer and less severe effects on somatic growth, whereas it does not affect cell proliferation within the brain. (c) 2006 Mosby, Inc. All rights reserved. https://portal.research.lu.se/en/publications/e8949203-28bb-4538-8163-c7b8000436e2 Elsevier 2006 eng pregnancy prenatal corticosteroids glucocorticoids neuroeenesis Fisher 344 rat Gynaecology, Obstetrics and Reproductive Medicine 1097-6868 7856202 000237331900024 33646116302 16579916 10.1016/j.ajog.2005.11.044 http://dx.doi.org/10.1016/j.ajog.2005.11.044 yes 194 5 1341 1346 https://www.scopus.com/pages/publications/33646116302 yes e8949203-28bb-4538-8163-c7b8000436e2 2016-04-01T12:22:07+02:00 2025-10-14T11:41:42+02:00 2016-04-01T12:22:07+02:00 20 1.1author exact 0000-0002-4775-872Xauthorexact0000-0002-4775-872X