1.1 2 info:srw/schema/1/mods-v3.3xml journalArticle published yes 5 Nguyen Tran Nguyen author Johan Hofkens author Ivan Scheblykin author d5fad9e8-59a6-42f4-9d92-56b857f3de06 Mikalai Kruk author Wim Dehaen author Chemical Physics v1000658 department Lund Laser Centre, LLC v1000184 department Several dendritic zinc(II)-porphyrins bearing carbazole units at the terminals have been prepared through click reaction of azide-substituted Zn-porphyrin precursors and carbazole-based alkynes under [Cu(NCCH3)(4)][PF6] catalysis. This family of new dendritic metalloporphyrins shows dual luminescence from both the upper S-2 and the lowest S-1 singlet states. The observed trends in the spectroscopic data and the photophysical properties of the dendrimers have been rationalized in terms of the type of meso-spacer between the macrocycle and dendritic shell. The key feature of the meso-spacer is proposed to be the degree of hindrance towards aryl ring rotation relative to the mean porphyrin plane. Based on the observed differences in the S1S0 fluorescence quantum yield on going from four- to five-coordinate dendrimers, it was shown that dendrimer architectures with all four meso-aryl spacers sterically hindered, are most appropriate to monitor processes related to axial ligation of the dendrimer core, since they provide larger luminescence response differences between the four-coordinate and five-coordinate forms. The blue S2S0 fluorescence quantum yield has been measured and the observed trend has been rationalized in terms of the S2S1 energy gap law. No significant differences were observed between the compounds either with different rotational degree of freedom of the meso-spacers or with different ligation states of the dendrimer core. https://portal.research.lu.se/en/publications/af334aa0-4dd6-4bd9-84e4-3a36ec08e182 Wiley-VCH Verlag 2014 eng Porphyrinoids Dendrimers Luminescence Photochemistry Fluorescence Click chemistry Atom and Molecular Physics and Optics Physical Chemistry (including Surface- and Colloid Chemistry) 1434-193X 4414302 000332161900022 84897658957 10.1002/ejoc.201301158 http://dx.doi.org/10.1002/ejoc.201301158 yes 2014 8 1766 1777 https://www.scopus.com/pages/publications/84897658957 The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Chemical Physics (S) (011001060) af334aa0-4dd6-4bd9-84e4-3a36ec08e182 2016-04-01T09:48:18+02:00 2026-05-29T11:56:18+02:00 2016-04-01T09:48:18+02:00 1 info:srw/schema/1/mods-v3.3xml journalArticle published yes 4 Dianxing Sun author Christine Roesler author Karin Kidd-Ljunggren author d22cb2ee-78e2-464a-b8ff-168dfff0135b Michael Nassal author Infection Medicine (BMC) v1000442 department Background & Aims: RNA interference (RNAi) may offer new treatment options for chronic hepatitis B. Replicating via an RNA intermediate, hepatitis B virus (HBV) is known to be principally vulnerable to RNAi. However, beyond delivery, the relevant issues of potential off-target effects, target site conservation in circulating HBV strains, and efficacy of RNAi itself have not systematically been addressed, nor can the different existing data be quantitatively compared. The aim of this study was to provide such information. Methods: To focus on the intracellular RNAi process itself and minimise other variables affecting overall RNAi efficacy, we used a robust co-transfection system to quantitatively assess the relative potencies of 21 small-hairpin (sh) RNA vectors, targeting conserved sites throughout the HBV genome, against viral RNAs, proteins, nucleocapsids, and secreted virions under standardised conditions. Results: The approach enabled a distinct efficacy ranking, with the six most potent shRNAs achieving >= 95% reductions in virion formation, sequence-specifically and without detectable interferon induction, yet by differentially affecting different steps. Efficacy correlated poorly with predictions and was not principally abolished by target structure. Sequence comparisons suggest that truly conserved, RNAi-targetable sequences comprise less than 500 nucleotides of the circulating HBV genomes. Conclusions: The HBV genome can harbour only a finite number of optimal target sites, but current predictions are poorly suited to constrain the number of possible candidates. However, the small size of the highly conserved sequence space suggests experimental identification as a viable option. (c) 2010 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved. https://portal.research.lu.se/en/publications/8643cf22-fb90-4d49-ac9d-c2c2d81d6b88 Elsevier 2010 eng HBV conservation Antiviral RNAi Hepatitis B virus RNA interference Structured RNAi targets Gastroenterology and Hepatology 0168-8278 1657419 000279705800008 77952425411 20400195 10.1016/j.jhep.2009.10.038 http://dx.doi.org/10.1016/j.jhep.2009.10.038 yes 52 6 817 826 https://www.scopus.com/pages/publications/77952425411 The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Division of Infection Medicine (SUS) (013008000) 8643cf22-fb90-4d49-ac9d-c2c2d81d6b88 2016-04-01T09:48:18+02:00 2025-10-14T09:16:01+02:00 2016-04-01T09:48:18+02:00 2 1.1id any "4414302 8643cf22-fb90-4d49-ac9d-c2c2d81d6b88"idany4414302 8643cf22-fb90-4d49-ac9d-c2c2d81d6b88