Identification of sex pheromone components of the Hessian fly, <i>Mayetiola destructor</i>.
Andersson, Martin N; Haftmann, Jenny; Stuart, Jeffrey J; Cambron, Sue E, et al. (2009). Identification of sex pheromone components of the Hessian fly, <i>Mayetiola destructor</i>.. Journal of Chemical Ecology, 35,, 81 - 95
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Published
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English
Authors:
Andersson, Martin N
;
Haftmann, Jenny
;
Stuart, Jeffrey J
;
Cambron, Sue E
, et al.
Department:
Pheromone Group
Research Group:
Pheromone Group
Abstract:
Coupled gas chromatographic (GC)–electroantennographic
detection (EAD) analyses of ovipositor
extract of calling Hessian fly, Mayetiola destructor, females
revealed that seven compounds elicited responses from
male antennae. Four of the compounds—(2S)-tridec-2-yl
acetate, (2S,10Z)-10-tridecen-2-yl acetate, (2S,10E)-10-
tridecen-2-yl acetate, and (2S,10E)-10-tridecen-2-ol—were
identified previously in female extracts. Two new EADactive
compounds, (2S,8Z,10E)-8,10-tridecadien-2-yl acetate
and (2S,8E,10E)-8,10-tridecadien-2-yl acetate, were identified
by GC–mass spectroscopy (MS) and the use of synthetic
reference samples. In a Y-tube bioassay, a five-component
blend (1 ng (2S)-tridec-2-yl acetate, 10 ng (2S,10E)-10-
tridecen-2-yl acetate, 1 ng (2S,10E)-10-tridecen-2-ol, 1 ng
(2S,8Z,10E)-8,10-tridecadien-2-yl acetate, and 1 ng
(2S,8E,10E)-8,10-tridecadien-2-yl acetate) was as attractive
to male Hessian flies as a similar amount of female extract
(with respect to the main compound, (2S,10E)-10-tridecen-
2-yl acetate). The five-component blend was more attractive
to male flies than a three-component blend lacking the two
dienes. Furthermore, the five-component blend was more
attractive than a blend with the same compounds but that
contained one tenth the concentration of (2S,8E,10E)-8,
10-tridecadien-2-yl acetate (more accurately mimicking the
ratios found in female extract). This suggests that the ratios
emitted by females might deviate from those in gland
extracts. In a field-trapping experiment, the five-component
blend applied to polyethylene cap dispensers in a 100:10 μg
ratio between the main component and each of the other
blend components attracted a significant number of male
Hessian flies. Also, a small-plot field test demonstrated the
attractiveness of the five-component blend to male Hessian
flies and suggests that this pheromone blend may be useful
for monitoring and predicting Hessian fly outbreaks in
agricultural systems.
Keywords:
10E)-8 ;
8Z ;
10E)-10-Tridecen-2-ol ;
10E)-10-tridecen-2-yl acetate ;
(2S ;
(2S)-Tridec-2-yl acetate ;
Sex pheromone ;
Diptera ;
Cecidomyiidae ;
Mayetiola destructor ;
Hessian fly ;
10-Tridecadien-2-yl acetate ;
8E ;
GC–EAD ;
Y-tube bioassay ;
Field trapping ;
Biological Sciences ;
Zoology
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