Purification of Leaf Mitochondria from Arabidopsis thaliana Using Percoll Density Gradients
Tran, Huy Cuong; Van Aken, Olivier (2022). Purification of Leaf Mitochondria from Arabidopsis thaliana Using Percoll Density Gradients In . Van Aken, Olivier; Rasmusson, Allan G. (Eds.). Plant Mitochondria : Methods and Protocols, 2363,, 1 - 12: Humana Press
Book Chapter
|
Published
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English
Authors:
Tran, Huy Cuong
;
Van Aken, Olivier
Editors:
Van Aken, Olivier
;
Rasmusson, Allan G.
Department:
Molecular Cell Biology
Plant Biology
Research Group:
Plant Biology
Abstract:
The study of plant mitochondria often requires isolation of mitochondria from plant tissues in intact and functional form. Here, we describe an effective procedure of mitochondrial isolation from leaf tissues and whole seedlings of the model dicot species Arabidopsis thaliana by using differential centrifugation and continuous Percoll density gradients.
Keywords:
Arabidopsis ;
Density gradients ;
Mitochondria ;
Mitochondrial isolation ;
Biochemistry and Molecular Biology ;
Cell Biology
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