Ten polymorphic microsatellite markers for Hieracium s.s. (Asteraceae)
Jönsson, Jane; Wellenreuther, Maren; Tyler, Torbjörn (2010). Ten polymorphic microsatellite markers for Hieracium s.s. (Asteraceae). Conservation Genetics Resources, 2, (1), 295 - 300
|
Published
|
English
Authors:
Jönsson, Jane
;
Wellenreuther, Maren
;
Tyler, Torbjörn
Department:
MEMEG
Botanical collections
Molecular Ecology and Evolution Lab
Research Group:
Molecular Ecology and Evolution Lab
Abstract:
Eight novel microsatellite markers were developed for Hieracium s.s. (excluding Pilosella) using a modified FIASCO protocol, and two out of nine microsatellites that were originally developed for Pilosella officinarum were optimised for Hieracium s.s.. All ten microsatellites consistently amplified loci from a wide range of representatives from north and central European species of various sections of the genus. When tested on 19 individuals, nine microsatellites were found to be highly polymorphic with 6–29 alleles, while one microsatellite marker was monomorphic. Cross-amplification showed that six of the ten microsatellites also amplified products in one taxon of Pilosella, P. floribunda × officinarum.
Keywords:
microsatellite marker ;
FIASCO ;
Hieracium ;
Pilosella ;
genetic variation ;
cross-amplification ;
Biological Sciences ;
Botany
Cite this