Respiration of [C-14] alanine by the ectomycorrhizal fungus Paxillus involutus
Chalot, Michel; Brun, Annick; Finlay, Roger D.; Söderström, Bengt (1994). Respiration of [C-14] alanine by the ectomycorrhizal fungus Paxillus involutus. FEMS Microbiology Letters, 121, (1), 87 - 91
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Published
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English
Authors:
Chalot, Michel
;
Brun, Annick
;
Finlay, Roger D.
;
Söderström, Bengt
Department:
MEMEG
Microbial Ecology
Research Group:
Microbial Ecology
Abstract:
The ectomycorrhizal fungus Paxillus involutus efficiently took up exogenously supplied [C-14]alanine and rapidly converted it to pyruvate, citrate, succinate, fumarate and to CO2, thus providing direct evidence for the utilisation of alanine as a respiratory substrate. [C-14]alanine was further actively metabolised to glutamate, glutamine and aspartate. Exposure to aminooxyacetate completely suppressed (CO2)-C-14 evolution and greatly reduced the flow of carbon from [C-14]alanine to tricarboxylic acid cycle intermediates and amino acids, suggesting that alanine aminotransferase plays a pivotal role in alanine metabolism in Paxillus involutus.
Keywords:
Amino acid respiration ;
Aminooxyacetate ;
[14C]alanine ;
Ectomycorrhizal fungus ;
Paxillus involutus ;
TCA cycle
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