Receptor for detection of a Type II sex pheromone in the winter moth <i>Operophtera brumata</i>

Abstract:
How signal diversity evolves under stabilizing selection in a pheromone-based mate recognition
system is a conundrum. Female moths produce two major types of sex pheromones, i.e., long-chain
acetates, alcohols and aldehydes (Type I) and polyenic hydrocarbons and epoxides (Type II), along
different biosynthetic pathways. Little is known on how male pheromone receptor (PR) genes evolved
to perceive the different pheromones. We report the identification of the first PR tuned to Type II
pheromones, namely ObruOR1 from the winter moth, Operophtera brumata (Geometridae). ObruOR1
clusters together with previously ligand-unknown orthologues in the PR subfamily for the ancestral
Type I pheromones, suggesting that O. brumata did not evolve a new type of PR to match the novel Type
II signal but recruited receptors within an existing PR subfamily. AsegOR3, the ObruOR1 orthologue
previously cloned from the noctuid Agrotis segetum that has Type I acetate pheromone components,
responded significantly to another Type II hydrocarbon, suggesting that a common ancestor with Type
I pheromones had receptors for both types of pheromones, a preadaptation for detection of Type II sex
pheromone.

Abstract in Undetermined

How signal diversity evolves under stabilizing selection in a pheromone-based mate recognition system is a conundrum. Female moths produce two major types of sex pheromones, i.e., long-chain acetates, alcohols and aldehydes (Type I) and polyenic hydrocarbons and epoxides (Type II), along different biosynthetic pathways. Little is known on how male pheromone receptor (PR) genes evolved to perceive the different pheromones. We report the identification of the first PR tuned to Type II pheromones, namely ObruOR1 from the winter moth, Operophtera brumata (Geometridae). ObruOR1 clusters together with previously ligand-unknown orthologues in the PR subfamily for the ancestral Type I pheromones, suggesting that O. brumata did not evolve a new type of PR to match the novel Type II signal but recruited receptors within an existing PR subfamily. AsegOR3, the ObruOR1 orthologue previously cloned from the noctuid Agrotis segetum that has Type I acetate pheromone components, responded significantly to another Type II hydrocarbon, suggesting that a common ancestor with Type I pheromones had receptors for both types of pheromones, a preadaptation for detection of Type II sex pheromone.