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Design, visualization, and utilization of enzyme microstructures built on solid surfaces

Gaspar, Szilveszter LU ; Schuhmann, W; Laurell, T and Csöregi, Elisabeth LU (2002) In Reviews in Analytical Chemistry 21(4). p.245-266
Abstract
Immobilized bioelements offer additional sensitivity and/or selectivity in analytical techniques, in biotechnological devices, and in different medical applications. Great research interest has recently been focused in all these fields on miniaturization techniques, special attention being accorded to the patterning of biomolecules (e.g. enzymes) on solid surfaces with micrometer resolution. Novel microanalysis systems consider the use of enzyme microstructures as e.g. part of mu- Total Analysis System platforms as biochemical microreactors or detection units. This mini-review highlights recent advances in the creation of enzyme microstructures by indirect methods (photolithography, microcontact printing. etc.) and active placement methods... (More)
Immobilized bioelements offer additional sensitivity and/or selectivity in analytical techniques, in biotechnological devices, and in different medical applications. Great research interest has recently been focused in all these fields on miniaturization techniques, special attention being accorded to the patterning of biomolecules (e.g. enzymes) on solid surfaces with micrometer resolution. Novel microanalysis systems consider the use of enzyme microstructures as e.g. part of mu- Total Analysis System platforms as biochemical microreactors or detection units. This mini-review highlights recent advances in the creation of enzyme microstructures by indirect methods (photolithography, microcontact printing. etc.) and active placement methods (electrospraying, microdispensing, etc.). Some key visualization techniques of enzyme microstructures (fluorescence microscopy, scanning electrochemical microscopy, etc.) are also mentioned together with examples of their applications or application possibilities. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
enzyme microstructures, scanning electrochemical microscopy, microdispenser
in
Reviews in Analytical Chemistry
volume
21
issue
4
pages
245 - 266
publisher
Freund Publishing House Ltd
external identifiers
  • wos:000182163400001
  • scopus:0036973144
ISSN
0048-752X
language
English
LU publication?
yes
id
1ea2e033-e9ff-41a1-a5d9-6c9405de3e59 (old id 313243)
date added to LUP
2007-08-20 14:30:47
date last changed
2017-01-01 06:53:38
@article{1ea2e033-e9ff-41a1-a5d9-6c9405de3e59,
  abstract     = {Immobilized bioelements offer additional sensitivity and/or selectivity in analytical techniques, in biotechnological devices, and in different medical applications. Great research interest has recently been focused in all these fields on miniaturization techniques, special attention being accorded to the patterning of biomolecules (e.g. enzymes) on solid surfaces with micrometer resolution. Novel microanalysis systems consider the use of enzyme microstructures as e.g. part of mu- Total Analysis System platforms as biochemical microreactors or detection units. This mini-review highlights recent advances in the creation of enzyme microstructures by indirect methods (photolithography, microcontact printing. etc.) and active placement methods (electrospraying, microdispensing, etc.). Some key visualization techniques of enzyme microstructures (fluorescence microscopy, scanning electrochemical microscopy, etc.) are also mentioned together with examples of their applications or application possibilities.},
  author       = {Gaspar, Szilveszter and Schuhmann, W and Laurell, T and Csöregi, Elisabeth},
  issn         = {0048-752X},
  keyword      = {enzyme microstructures,scanning electrochemical microscopy,microdispenser},
  language     = {eng},
  number       = {4},
  pages        = {245--266},
  publisher    = {Freund Publishing House Ltd},
  series       = {Reviews in Analytical Chemistry},
  title        = {Design, visualization, and utilization of enzyme microstructures built on solid surfaces},
  volume       = {21},
  year         = {2002},
}