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Immunological alteration and changes of gut microbiota after dextran sulfate sodium (DSS) administration in mice.

Håkansson, A; Tormo-Badia, Neivis LU ; Baridi, Ajoeb LU ; Xu, J; Molin, G; Hagslätt, M-L; Karlsson, C; Jeppsson, Bengt LU ; Cilio, Corrado LU and Ahrné, S (2015) In Clinical and Experimental Medicine 15(1). p.107-120
Abstract
Ulcerative colitis (UC) is characterized by chronic inflammation of the colonic mucosa. Administration of dextran sulfate sodium (DSS) to animals is a frequently used model to mimic human colitis. Deregulation of the immune response to the enteric microflora or pathogens as well as increased intestinal permeability have been proposed as disease-driving mechanisms. To enlarge the understanding of the pathogenesis, we have studied the effect of DSS on the immune system and gut microbiota in mice. Intestinal inflammation was verified through histological evaluation and myeloperoxidase activity. Immunological changes were assessed by flow cytometry in spleen, Peyer's patches and mesenteric lymph nodes and through multiplex cytokine profiling.... (More)
Ulcerative colitis (UC) is characterized by chronic inflammation of the colonic mucosa. Administration of dextran sulfate sodium (DSS) to animals is a frequently used model to mimic human colitis. Deregulation of the immune response to the enteric microflora or pathogens as well as increased intestinal permeability have been proposed as disease-driving mechanisms. To enlarge the understanding of the pathogenesis, we have studied the effect of DSS on the immune system and gut microbiota in mice. Intestinal inflammation was verified through histological evaluation and myeloperoxidase activity. Immunological changes were assessed by flow cytometry in spleen, Peyer's patches and mesenteric lymph nodes and through multiplex cytokine profiling. In addition, quantification of the total amount of bacteria on colonic mucosa as well as the total amount of lactobacilli, Akkermansia, Desulfovibrio and Enterobacteriaceae was performed by the use of quantitative PCR. Diversity and community structure were analysed by terminal restriction fragment length polymorphism (T-RFLP) patterns, and principal component analysis was utilized on immunological and T-RFLP patterns. DSS-induced colitis show clinical and histological similarities to UC. The composition of the colonic microflora was profoundly changed and correlated with several alterations of the immune system. The results demonstrate a relationship between multiple immunological changes and alterations of the gut microbiota after DSS administration. These data highlight and improve the definition of the immunological basis of the disease and suggest a role for dysregulation of the gut microbiota in the pathogenesis of colitis. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Clinical and Experimental Medicine
volume
15
issue
1
pages
107 - 120
publisher
Springer
external identifiers
  • pmid:24414342
  • wos:000348977100012
  • scopus:84902262539
ISSN
1591-9528
DOI
10.1007/s10238-013-0270-5
language
English
LU publication?
yes
id
4cf3c45f-72c3-4bce-a34f-9208f274152d (old id 4291579)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/24414342?dopt=Abstract
date added to LUP
2014-02-06 23:33:53
date last changed
2017-10-01 03:16:26
@article{4cf3c45f-72c3-4bce-a34f-9208f274152d,
  abstract     = {Ulcerative colitis (UC) is characterized by chronic inflammation of the colonic mucosa. Administration of dextran sulfate sodium (DSS) to animals is a frequently used model to mimic human colitis. Deregulation of the immune response to the enteric microflora or pathogens as well as increased intestinal permeability have been proposed as disease-driving mechanisms. To enlarge the understanding of the pathogenesis, we have studied the effect of DSS on the immune system and gut microbiota in mice. Intestinal inflammation was verified through histological evaluation and myeloperoxidase activity. Immunological changes were assessed by flow cytometry in spleen, Peyer's patches and mesenteric lymph nodes and through multiplex cytokine profiling. In addition, quantification of the total amount of bacteria on colonic mucosa as well as the total amount of lactobacilli, Akkermansia, Desulfovibrio and Enterobacteriaceae was performed by the use of quantitative PCR. Diversity and community structure were analysed by terminal restriction fragment length polymorphism (T-RFLP) patterns, and principal component analysis was utilized on immunological and T-RFLP patterns. DSS-induced colitis show clinical and histological similarities to UC. The composition of the colonic microflora was profoundly changed and correlated with several alterations of the immune system. The results demonstrate a relationship between multiple immunological changes and alterations of the gut microbiota after DSS administration. These data highlight and improve the definition of the immunological basis of the disease and suggest a role for dysregulation of the gut microbiota in the pathogenesis of colitis.},
  author       = {Håkansson, A and Tormo-Badia, Neivis and Baridi, Ajoeb and Xu, J and Molin, G and Hagslätt, M-L and Karlsson, C and Jeppsson, Bengt and Cilio, Corrado and Ahrné, S},
  issn         = {1591-9528},
  language     = {eng},
  number       = {1},
  pages        = {107--120},
  publisher    = {Springer},
  series       = {Clinical and Experimental Medicine},
  title        = {Immunological alteration and changes of gut microbiota after dextran sulfate sodium (DSS) administration in mice.},
  url          = {http://dx.doi.org/10.1007/s10238-013-0270-5},
  volume       = {15},
  year         = {2015},
}