@article{7359e723-7c16-413b-b32b-558bb9f031b9,
  abstract     = {{<p>Early diagnosis of colorectal cancer (CRC) is crucial for successful treatment and mortality reduction. In this regard, blood-based tests play an indispensable role. Current research is focused on molecules actively secreted by tumor cells into small extracellular vesicles (EVs). This four-phase study included 613 CRC patients, 446 controls, and 120 precancerous lesions. High-throughput transcriptome profiling of small EVs was performed on samples from 100 CRC patients and 50 controls, followed by extensive validation using reverse transcription quantitative polymerase chain reaction. Diagnostic panels were developed via logistic regression and further characterized by enrolling samples from gastric cancer patients, CRC patients before/after surgery, and samples of tumor tissues/adjacent mucosa. We identified 17 molecules significantly elevated in CRC, with the highest levels in metastatic cases. Additionally, seven of them differentiated controls from precancerous lesions. Two diagnostic panels were developed, enabling early CRC detection with high sensitivity and specificity, outperforming the fecal occult blood test. Furthermore, six molecules were differentially expressed between tumor tissue and mucosa, while seven EV-enriched molecules decreased significantly after surgery. These findings highlight EVs as key reservoirs of CRC-associated molecules and a promising source of biomarkers.</p>}},
  author       = {{Vychytilova-Faltejskova, Petra and Pavlikova, Marketa and Pifkova, Lucie and Jugas, Robin and Machackova, Tana and Radova, Lenka and Sachlova, Milana and Bartosova, Renata and Samoilenko, Tetiana and Feckova, Zuzana and Orlickova, Jana and Slamova, Erika and Ponechal Michu, Elleni and Al Tukmachi, Dagmar and Ruckova, Michaela and Vodinska, Martina and Kotoucek, Jan and Catela Ivkovic, Tina and Boudna, Marie and Bohovicova, Lucia and Stanek, Teodor and Halamkova, Jana and Svoboda, Martin and Prochazka, Vladimir and Grolich, Tomas and Kala, Zdenek and Slaby, Ondrej}},
  issn         = {{1574-7891}},
  keywords     = {{Humans; Colorectal Neoplasms/genetics; Extracellular Vesicles/metabolism; Male; Female; RNA, Messenger/genetics; Early Detection of Cancer/methods; Middle Aged; RNA, Long Noncoding/genetics; Gene Expression Profiling; Aged; Biomarkers, Tumor/genetics; Gene Expression Regulation, Neoplastic; Case-Control Studies}},
  language     = {{eng}},
  number       = {{11}},
  pages        = {{3445--3462}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{Molecular Oncology}},
  title        = {{Comprehensive profiling of lncRNAs and mRNAs enriched in small extracellular vesicles for early noninvasive detection of colorectal cancer : diagnostic panel assembly and extensive validation}},
  url          = {{http://dx.doi.org/10.1002/1878-0261.70086}},
  doi          = {{10.1002/1878-0261.70086}},
  volume       = {{19}},
  year         = {{2025}},
}