@article{759af967-b72e-4754-8875-d91852292b2f,
  abstract     = {{<p>Background: Expression of CD25, the IL-2 receptor alpha chain, on human mast cells is primarily associated with aberrant mast cells in clonal mast cell disorders. However, the regulation of CD25 expression in normal, mature tissue-resident mast cells remains poorly understood. Objective: IL-33 is a key modulator of immune responses, including in lung inflammatory conditions. Because mast cells are prominent IL-33 receptor–expressing cells, we investigated the effect of IL-33 on CD25 expression in purified human lung mast cells (HLMCs). Methods: Purified HLMCs were stimulated with IL-33 and the transcriptional responses measured by RNA sequencing. The expression of the IL-2 receptor subunits CD25 (IL2RA), CD122 (IL2RB), and CD132 (IL2RG) was quantified by real-time quantitative PCR and flow cytometry. IL2RA expression was further examined in publicly available single-cell RNA sequencing datasets, and in situ CD25 protein expression on HLMCs was assessed in human lung tissue by immunofluorescence staining. Results: IL-33 robustly induced the expression of CD25 and CD132 in HLMCs without a corresponding upregulation of CD122, resulting in absent IL-2–mediated signaling despite enhanced IL-2 binding via CD25. Single-cell RNA sequencing data identified IL2RA+ mast cells as a distinct subpopulation with enriched IL-33 response signatures and upregulation of genes linked to immune signaling and inflammatory pathways. CD25-positive HLMCs were also detected in situ, displaying substantial heterogeneity in expression levels and spatial distribution. Conclusions: CD25 expression in HLMCs is upregulated by IL-33 and is dynamically regulated in human lung tissues.</p>}},
  author       = {{Gong, Yitao and Atanasoai, Ionut and Siddhuraj, Premkumar and Johnsson, Anna Karin and Peng, Yueling and Al-Ameri, Mamdoh and Sachs, Erik and Vali, Kasra and Adner, Mikael and Säfholm, Jesper and Erjefält, Jonas S. and Nilsson, Gunnar P. and Rönnberg, Elin}},
  issn         = {{0091-6749}},
  keywords     = {{CD25; COPD; IL-2 receptor; IL-33; Lung mast cells; pulmonary emphysema}},
  language     = {{eng}},
  number       = {{6}},
  pages        = {{1442--1449}},
  publisher    = {{Elsevier}},
  series       = {{Journal of Allergy and Clinical Immunology}},
  title        = {{Expression of CD25 in human lung mast cells and its regulation by IL-33}},
  url          = {{http://dx.doi.org/10.1016/j.jaci.2026.03.003}},
  doi          = {{10.1016/j.jaci.2026.03.003}},
  volume       = {{157}},
  year         = {{2026}},
}