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Responding double-porous lipid membrane: Lyotropic phases in a polymer scaffold

Åberg, Christoffer LU ; Pairin, Cecile LU ; Costa, Fatima LU and Sparr, Emma LU (2008) In Biochimica et Biophysica Acta - Biomembranes 1778(2). p.549-558
Abstract
The large osmotic gradient over the outermost layer of human skin implies major structural changes along the gradient, which in turn affects transport. In particular, the possibility of phase changes introduces a non-linear element to the transport behaviour. We present a novel model membrane system to be used for studying these transport mechanisms, where we use a hydrophobic porous polymer membrane as a scaffold for lipid lyotropic phases. The polymer membrane provides mechanical robustness, but also prevents defects of the lipid lyotropic phases, and it can induce an orientation of anisotropic phases. We study the location, structure and phase behaviour of the confined phases. It is shown that this model membrane system allow for... (More)
The large osmotic gradient over the outermost layer of human skin implies major structural changes along the gradient, which in turn affects transport. In particular, the possibility of phase changes introduces a non-linear element to the transport behaviour. We present a novel model membrane system to be used for studying these transport mechanisms, where we use a hydrophobic porous polymer membrane as a scaffold for lipid lyotropic phases. The polymer membrane provides mechanical robustness, but also prevents defects of the lipid lyotropic phases, and it can induce an orientation of anisotropic phases. We study the location, structure and phase behaviour of the confined phases. It is shown that this model membrane system allow for accurate measurements of transport through lipid membranes in the presence of different osmotic gradients. A theoretical description is evaluated and shows that this phenomenon can be understood in terms of the proposed mechanism of phase changes. The novel double-porous lipid membrane constitutes a mechanically robust system for studies in aligned systems, which is generally very difficult to

achieve. This could have large implications for studies of transport processes in, e.g. skin and other biomembrane model systems. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Stratum corneum, Phase transformation, Diffusive transport, Responding membrane, Osmotic gradient, Permeability
in
Biochimica et Biophysica Acta - Biomembranes
volume
1778
issue
2
pages
549 - 558
publisher
Elsevier
external identifiers
  • pmid:18067852
  • wos:000253269700020
  • scopus:38349001116
ISSN
0005-2736
DOI
10.1016/j.bbamem.2007.10.020
language
English
LU publication?
yes
id
1c12d411-6806-412f-8d65-41fc761168a8 (old id 784242)
date added to LUP
2007-12-20 17:12:27
date last changed
2017-01-01 05:43:06
@article{1c12d411-6806-412f-8d65-41fc761168a8,
  abstract     = {The large osmotic gradient over the outermost layer of human skin implies major structural changes along the gradient, which in turn affects transport. In particular, the possibility of phase changes introduces a non-linear element to the transport behaviour. We present a novel model membrane system to be used for studying these transport mechanisms, where we use a hydrophobic porous polymer membrane as a scaffold for lipid lyotropic phases. The polymer membrane provides mechanical robustness, but also prevents defects of the lipid lyotropic phases, and it can induce an orientation of anisotropic phases. We study the location, structure and phase behaviour of the confined phases. It is shown that this model membrane system allow for accurate measurements of transport through lipid membranes in the presence of different osmotic gradients. A theoretical description is evaluated and shows that this phenomenon can be understood in terms of the proposed mechanism of phase changes. The novel double-porous lipid membrane constitutes a mechanically robust system for studies in aligned systems, which is generally very difficult to<br/><br>
achieve. This could have large implications for studies of transport processes in, e.g. skin and other biomembrane model systems.},
  author       = {Åberg, Christoffer and Pairin, Cecile and Costa, Fatima and Sparr, Emma},
  issn         = {0005-2736},
  keyword      = {Stratum corneum,Phase transformation,Diffusive transport,Responding membrane,Osmotic gradient,Permeability},
  language     = {eng},
  number       = {2},
  pages        = {549--558},
  publisher    = {Elsevier},
  series       = {Biochimica et Biophysica Acta - Biomembranes},
  title        = {Responding double-porous lipid membrane: Lyotropic phases in a polymer scaffold},
  url          = {http://dx.doi.org/10.1016/j.bbamem.2007.10.020},
  volume       = {1778},
  year         = {2008},
}