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Proton Nuclear Magnetic Resonance Relaxation in Aqueous Sugar Solutions : Can Low-Field Nuclear Magnetic Resonance Relaxation Measurements Differentiate between “Bound” and “Free” Water?

Gowda, Vasantha LU ; Argatov, Ivan ; Söderman, Olle LU and Kocherbitov, Vitaly LU (2026) In ACS Physical Chemistry Au 6(1). p.196-206
Abstract

Understanding water interactions in complex systems is crucial, as they play a key role in fields such as biochemistry, pharmaceutical formulations, and food science. Nuclear magnetic resonance (NMR) relaxation measurements have become one of the widely used methods to visualize various water characteristics owing to their noninvasive nature and ease of use. However, unambiguous data interpretation can be challenging and potentially misleading if not carefully analyzed. One such example is the observation of multiple relaxation times, which is often linked to different water types such as “bound” and “free”. In this paper, we present a new approach for the interpretation of proton NMR relaxation data using a second-order reaction... (More)

Understanding water interactions in complex systems is crucial, as they play a key role in fields such as biochemistry, pharmaceutical formulations, and food science. Nuclear magnetic resonance (NMR) relaxation measurements have become one of the widely used methods to visualize various water characteristics owing to their noninvasive nature and ease of use. However, unambiguous data interpretation can be challenging and potentially misleading if not carefully analyzed. One such example is the observation of multiple relaxation times, which is often linked to different water types such as “bound” and “free”. In this paper, we present a new approach for the interpretation of proton NMR relaxation data using a second-order reaction kinetics-based model. The case of first-order asymptotic analysis considering fast proton exchange is shown to be of particular relevance. The presented theory is tested using a series of sucrose–water and sucrose-D2O systems with varying sucrose content. The comparison of these systems reveals a biexponential behavior in both T1 and T2 relaxation times. These observations are interpreted by considering both nonexchangeable and exchangeable protons in the system, with the corresponding contribution coefficients following trends consistent with the concentrations of these proton types.

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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
low-field NMR, proton NMR relaxation, solute−solvent interactions, sucrose−water system, water dynamics
in
ACS Physical Chemistry Au
volume
6
issue
1
pages
11 pages
publisher
The American Chemical Society (ACS)
external identifiers
  • scopus:105029553313
  • pmid:41624726
ISSN
2694-2445
DOI
10.1021/acsphyschemau.5c00108
language
English
LU publication?
yes
id
a234a129-eb0c-4f99-b509-0f6ce528c649
date added to LUP
2026-03-02 11:33:40
date last changed
2026-06-09 01:05:44
@article{a234a129-eb0c-4f99-b509-0f6ce528c649,
  abstract     = {{<p>Understanding water interactions in complex systems is crucial, as they play a key role in fields such as biochemistry, pharmaceutical formulations, and food science. Nuclear magnetic resonance (NMR) relaxation measurements have become one of the widely used methods to visualize various water characteristics owing to their noninvasive nature and ease of use. However, unambiguous data interpretation can be challenging and potentially misleading if not carefully analyzed. One such example is the observation of multiple relaxation times, which is often linked to different water types such as “bound” and “free”. In this paper, we present a new approach for the interpretation of proton NMR relaxation data using a second-order reaction kinetics-based model. The case of first-order asymptotic analysis considering fast proton exchange is shown to be of particular relevance. The presented theory is tested using a series of sucrose–water and sucrose-D<sub>2</sub>O systems with varying sucrose content. The comparison of these systems reveals a biexponential behavior in both T<sub>1</sub> and T<sub>2</sub> relaxation times. These observations are interpreted by considering both nonexchangeable and exchangeable protons in the system, with the corresponding contribution coefficients following trends consistent with the concentrations of these proton types.</p>}},
  author       = {{Gowda, Vasantha and Argatov, Ivan and Söderman, Olle and Kocherbitov, Vitaly}},
  issn         = {{2694-2445}},
  keywords     = {{low-field NMR; proton NMR relaxation; solute−solvent interactions; sucrose−water system; water dynamics}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{196--206}},
  publisher    = {{The American Chemical Society (ACS)}},
  series       = {{ACS Physical Chemistry Au}},
  title        = {{Proton Nuclear Magnetic Resonance Relaxation in Aqueous Sugar Solutions : Can Low-Field Nuclear Magnetic Resonance Relaxation Measurements Differentiate between “Bound” and “Free” Water?}},
  url          = {{http://dx.doi.org/10.1021/acsphyschemau.5c00108}},
  doi          = {{10.1021/acsphyschemau.5c00108}},
  volume       = {{6}},
  year         = {{2026}},
}