@article{aa2bd17d-b04c-4d0f-9947-6ddd3c6f3341,
  abstract     = {{<p>Colorectal carcinoma (CRC) results from the accumulation of genetic mutations and alterations in signaling pathways. KRAS is mutated in 40% of CRC cases and is involved in increased tumor cells proliferation and survival. Although KRAS mutations are a dominant event in CRC tumorigenesis, increased wild-type KRAS expression has a similar effect on accelerated tumor growth. In this study, we investigated the KRAS status in correlation with clinicopathological features in sporadic CRC and more importantly the role of let-7a-5p and miR-544a-3p in the regulation of wild-type KRAS protein expression in the tumor center (T1) and invasive tumor front (T2). Analysis showed that 39.1% of tumor samples had KRAS mutations. In wild-type KRAS tumors, 62.0% were positive for KRAS protein expression and there was a higher percentage of KRAS-positive tumor cells and a higher intensity of immunohistochemical reaction in T2 than in T1 samples. This could not be attributed to differences in KRAS mRNA levels, suggesting regulation via miR-544a-3p expression which was significantly decreased in T2 samples. Furthermore, we demonstrated that tumor samples carrying the KRAS-LCS6 variant allele had significantly higher protein expression of the wild-type KRAS. Our results suggest the role of the KRAS-LCS6 polymorphism and miR-544a-3p expression in the regulation of wild-type KRAS protein expression in sporadic CRC.</p>}},
  author       = {{Marinović, Sonja and Škrtić, Anita and Catela Ivković, Tina and Poljak, Mirko and Kapitanović, Sanja}},
  issn         = {{0914-7470}},
  keywords     = {{Colon adenocarcinoma; Immunohistochemistry; KRAS; Let-7a; miR-544a}},
  language     = {{eng}},
  number       = {{5}},
  pages        = {{1455--1465}},
  publisher    = {{Springer}},
  series       = {{Human Cell}},
  title        = {{Regulation of KRAS protein expression by miR-544a and KRAS-LCS6 polymorphism in wild-type KRAS sporadic colon adenocarcinoma}},
  url          = {{http://dx.doi.org/10.1007/s13577-021-00576-2}},
  doi          = {{10.1007/s13577-021-00576-2}},
  volume       = {{34}},
  year         = {{2021}},
}