Epstein-Barr virus binding to virus-carrying cell lines is enhanced in the presence of C3 and C3d
(1984) In Journal of General Virology 65 ( Pt 3). p.13-507- Abstract
- The relationship between the receptors for the Epstein-Barr virus (EBV) and the C3d fragment of complement was investigated at the molecular level. In the presence of cell-bound C3, virus binding was enhanced in EBV genome-carrying lines. An identical effect could be elicited by C3d at one-quarter the weight amount; C3b and methylamine-treated C3 had no effect on virus binding. The minimum concentration of C3 which produced significant enhancement was 25 micrograms/ml. Virus binding increases were observed only after 20 min of complement-cell co-incubation. The response was not noted with EBV-negative lines and was independent of virus strain assayed (B95-8 and P3HR-1). These studies suggest that the binding sites for the two moieties are... (More)
- The relationship between the receptors for the Epstein-Barr virus (EBV) and the C3d fragment of complement was investigated at the molecular level. In the presence of cell-bound C3, virus binding was enhanced in EBV genome-carrying lines. An identical effect could be elicited by C3d at one-quarter the weight amount; C3b and methylamine-treated C3 had no effect on virus binding. The minimum concentration of C3 which produced significant enhancement was 25 micrograms/ml. Virus binding increases were observed only after 20 min of complement-cell co-incubation. The response was not noted with EBV-negative lines and was independent of virus strain assayed (B95-8 and P3HR-1). These studies suggest that the binding sites for the two moieties are distinct, although they both involve the same cell surface complex. The two receptors are believed to display cooperativity. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3965074
- author
- Wells, A. ; Koide, N. ; Eggertsen, G. ; Lundwall, Åke LU ; Godal, T. ; Steen, H. B. and Klein, G.
- publishing date
- 1984
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Complement/*analysis, Receptors, Kinetics, Humans, Human/*metabolism, Herpesvirus 4, Complement C3d, Cell Line, Complement C3/*metabolism, Virus/*analysis, Research Support, Non-U.S. Gov't, U.S. Gov't, P.H.S.
- in
- Journal of General Virology
- volume
- 65 ( Pt 3)
- pages
- 13 - 507
- publisher
- Microbiology Society
- external identifiers
-
- scopus:0021355720
- ISSN
- 1465-2099
- language
- English
- LU publication?
- no
- id
- e2c03fa6-0d1e-4b7f-bfbd-ade9ae01db80 (old id 3965074)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=6321638
- date added to LUP
- 2016-04-04 14:05:49
- date last changed
- 2021-01-03 11:23:10
@article{e2c03fa6-0d1e-4b7f-bfbd-ade9ae01db80, abstract = {{The relationship between the receptors for the Epstein-Barr virus (EBV) and the C3d fragment of complement was investigated at the molecular level. In the presence of cell-bound C3, virus binding was enhanced in EBV genome-carrying lines. An identical effect could be elicited by C3d at one-quarter the weight amount; C3b and methylamine-treated C3 had no effect on virus binding. The minimum concentration of C3 which produced significant enhancement was 25 micrograms/ml. Virus binding increases were observed only after 20 min of complement-cell co-incubation. The response was not noted with EBV-negative lines and was independent of virus strain assayed (B95-8 and P3HR-1). These studies suggest that the binding sites for the two moieties are distinct, although they both involve the same cell surface complex. The two receptors are believed to display cooperativity.}}, author = {{Wells, A. and Koide, N. and Eggertsen, G. and Lundwall, Åke and Godal, T. and Steen, H. B. and Klein, G.}}, issn = {{1465-2099}}, keywords = {{Complement/*analysis; Receptors; Kinetics; Humans; Human/*metabolism; Herpesvirus 4; Complement C3d; Cell Line; Complement C3/*metabolism; Virus/*analysis; Research Support; Non-U.S. Gov't; U.S. Gov't; P.H.S.}}, language = {{eng}}, pages = {{13--507}}, publisher = {{Microbiology Society}}, series = {{Journal of General Virology}}, title = {{Epstein-Barr virus binding to virus-carrying cell lines is enhanced in the presence of C3 and C3d}}, url = {{http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=6321638}}, volume = {{65 ( Pt 3)}}, year = {{1984}}, }