Ion exchange chromatography of glucosamine and galactosamine in microgram amounts with quantitative determination and specific radioactivity assay
(1972) In Biochimica et Biophysica Acta. General Subjects 264(3). p.411-417- Abstract
A rapid method for the separation and quantitative determination, including radioassay, of glucosamine and galactosamine in microgram amounts is described. It is based upon the use of a column of Aminex A-5 ion exchange resin eluted with a phosphate buffer at pH 7.00 and 60 °C. From each fraction half the volume is used for a scaled down Elson-Morgan procedure and other half for liquid scintillation counting. Amounts of about 0.01-1 μmole of hexosamine may be separated and determined.
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https://lup.lub.lu.se/record/586a4d48-6d98-4dda-aab3-ca3ab2befddc
- author
- Lohmander, Stefan LU
- organization
- publishing date
- 1972-05-16
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biochimica et Biophysica Acta. General Subjects
- volume
- 264
- issue
- 3
- pages
- 7 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:0015512384
- pmid:5027625
- ISSN
- 0304-4165
- DOI
- 10.1016/0304-4165(72)90003-7
- language
- English
- LU publication?
- yes
- id
- 586a4d48-6d98-4dda-aab3-ca3ab2befddc
- date added to LUP
- 2016-05-04 12:36:47
- date last changed
- 2024-01-04 02:33:20
@article{586a4d48-6d98-4dda-aab3-ca3ab2befddc, abstract = {{<p>A rapid method for the separation and quantitative determination, including radioassay, of glucosamine and galactosamine in microgram amounts is described. It is based upon the use of a column of Aminex A-5 ion exchange resin eluted with a phosphate buffer at pH 7.00 and 60 °C. From each fraction half the volume is used for a scaled down Elson-Morgan procedure and other half for liquid scintillation counting. Amounts of about 0.01-1 μmole of hexosamine may be separated and determined.</p>}}, author = {{Lohmander, Stefan}}, issn = {{0304-4165}}, language = {{eng}}, month = {{05}}, number = {{3}}, pages = {{411--417}}, publisher = {{Elsevier}}, series = {{Biochimica et Biophysica Acta. General Subjects}}, title = {{Ion exchange chromatography of glucosamine and galactosamine in microgram amounts with quantitative determination and specific radioactivity assay}}, url = {{http://dx.doi.org/10.1016/0304-4165(72)90003-7}}, doi = {{10.1016/0304-4165(72)90003-7}}, volume = {{264}}, year = {{1972}}, }