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Pubertal mammary gland development : Elucidation of in vivo morphogenesis using Murine models

McBryan, Jean and Howlin, Jillian LU (2017) In Methods in Molecular Biology 1501. p.77-114
Abstract

During the past 25 years, the combination of increasingly sophisticated gene targeting technology with transplantation techniques has allowed researchers to address a wide array of questions about postnatal mammary gland development. These in turn have significantly contributed to our knowledge of other branched epithelial structures. This review chapter highlights a selection of the mouse models exhibiting a pubertal mammary gland phenotype with a focus on how they have contributed to our overall understanding of in vivo mammary morphogenesis. We discuss mouse models that have enabled us to assign functions to particular genes and proteins and, more importantly, have determined when and where these factors are required for completion... (More)

During the past 25 years, the combination of increasingly sophisticated gene targeting technology with transplantation techniques has allowed researchers to address a wide array of questions about postnatal mammary gland development. These in turn have significantly contributed to our knowledge of other branched epithelial structures. This review chapter highlights a selection of the mouse models exhibiting a pubertal mammary gland phenotype with a focus on how they have contributed to our overall understanding of in vivo mammary morphogenesis. We discuss mouse models that have enabled us to assign functions to particular genes and proteins and, more importantly, have determined when and where these factors are required for completion of ductal outgrowth and branch patterning. The reason for the success of the mouse mammary gland model is undoubtedly the suitability of the postnatal mammary gland to experimental manipulation. The gland itself is very amenable to investigation and the combination of genetic modification with accessibility to the tissue has allowed an impressive number of studies to inform biology. Excision of the rudimentary epithelial structure postnatally allows genetically modified tissue to be readily transplanted into wild type stroma or vice versa, and has thus defined the contribution of each compartment to particular phenotypes. Similarly, whole gland transplantation has been used to definitively discern local effects from indirect systemic effects of various growth factors and hormones. While appreciative of the power of these tools and techniques, we are also cognizant of some of their limitations, and we discuss some shortcomings and future strategies that can overcome them.

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author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Branch patterning, Breast, Cell polarity, Cell-cell adhesion, Development, Epithelial branching, Extracellular matrix, In vivo morphogenesis, Knockout, Mammary gland, Mammary gland transplantation, Mammary stem cells, Mouse models, Pubertal mammary gland development, Terminal endbud, Transgenic
in
Methods in Molecular Biology
volume
1501
pages
38 pages
publisher
Humana Press
external identifiers
  • Scopus:84994302392
ISSN
10643745
DOI
10.1007/978-1-4939-6475-8_3
language
English
LU publication?
yes
id
ffb81ece-a8d3-446c-b3bf-972a8687ca8c
date added to LUP
2017-03-20 14:38:37
date last changed
2017-03-20 14:38:37
@inbook{ffb81ece-a8d3-446c-b3bf-972a8687ca8c,
  abstract     = {<p>During the past 25 years, the combination of increasingly sophisticated gene targeting technology with transplantation techniques has allowed researchers to address a wide array of questions about postnatal mammary gland development. These in turn have significantly contributed to our knowledge of other branched epithelial structures. This review chapter highlights a selection of the mouse models exhibiting a pubertal mammary gland phenotype with a focus on how they have contributed to our overall understanding of in vivo mammary morphogenesis. We discuss mouse models that have enabled us to assign functions to particular genes and proteins and, more importantly, have determined when and where these factors are required for completion of ductal outgrowth and branch patterning. The reason for the success of the mouse mammary gland model is undoubtedly the suitability of the postnatal mammary gland to experimental manipulation. The gland itself is very amenable to investigation and the combination of genetic modification with accessibility to the tissue has allowed an impressive number of studies to inform biology. Excision of the rudimentary epithelial structure postnatally allows genetically modified tissue to be readily transplanted into wild type stroma or vice versa, and has thus defined the contribution of each compartment to particular phenotypes. Similarly, whole gland transplantation has been used to definitively discern local effects from indirect systemic effects of various growth factors and hormones. While appreciative of the power of these tools and techniques, we are also cognizant of some of their limitations, and we discuss some shortcomings and future strategies that can overcome them.</p>},
  author       = {McBryan, Jean and Howlin, Jillian},
  issn         = {10643745},
  keyword      = {Branch patterning,Breast,Cell polarity,Cell-cell adhesion,Development,Epithelial branching,Extracellular matrix,In vivo morphogenesis,Knockout,Mammary gland,Mammary gland transplantation,Mammary stem cells,Mouse models,Pubertal mammary gland development,Terminal endbud,Transgenic},
  language     = {eng},
  pages        = {77--114},
  publisher    = {Humana Press},
  series       = {Methods in Molecular Biology},
  title        = {Pubertal mammary gland development : Elucidation of in vivo morphogenesis using Murine models},
  url          = {http://dx.doi.org/10.1007/978-1-4939-6475-8_3},
  volume       = {1501},
  year         = {2017},
}