@misc{9236343,
  abstract     = {{Thioflavin T (ThT) is widely used as a fluorescent probe for amyloid fibrils, yet its fluorescence can also be enhanced in non-fibrillar systems. Here, the photophysical behaviour of ThT was investigated in sodium dodecyl sulfate (SDS), sucrose laurate (SL), sodium caprate (C10), and lanreotide-containing assemblies across acetate (pH 4.0), phosphate (pH 7.4), and Gly-Gly (pH 8.5) buffers using UV–visible absorption spectroscopy, steady-state fluorescence spectroscopy, time-correlated single-photon counting (TCSPC), and dynamic light scattering.
SDS and C10 produced concentration-dependent red shifts in the ThT absorption spectra of ~412 to 429 nm, while SL generated a red shift in the ThT absorption spectra of ~412 to 426 nm which was not expected and the strongest fluorescence enhancement. Lanreotide-containing systems displayed a distinct excitation shift from approximately 424 to 440 nm and enhanced fluorescence intensity. Despite these pronounced steady-state spectral changes, SDS, C10 and SL systems exhibited short fluorescence lifetimes (0.05–0.26 ns), indicating microenvironmental confinement rather than rigid amyloid-like immobilization. These findings demonstrate that ThT responds to a combination of electrostatic interactions, hydrophobic partitioning, and supramolecular organization, highlighting the importance of combining steady-state and time-resolved fluorescence measurements when interpreting ThT responses in complex molecular environments.}},
  author       = {{Coker, Ewura Abena}},
  language     = {{eng}},
  note         = {{Student Paper}},
  title        = {{Exploration of the Thioflavin T in complex systems for peptide drug delivery – Mechanistic Mapping of Thioflavin T Matrix Effects}},
  year         = {{2026}},
}