@misc{9237032,
  abstract     = {{The most prevalent method of peptide production, solid-phase peptide synthesis (SPPS), generates large amounts of hazardous solvent waste, typically DMF. This waste, combined with the use of the insidious PFAS chemical TFA for side chain PG removal and final resin cleavage, has prompted research into PFAS-free and aqueous solid-phase peptide synthesis (ASPPS) of linear peptides. With the growth of branched chain GLP-1 medications, ASPPS exploration of complex peptides is essential.
The aim of this project was combining ASPPS of a ε-Lys peptide, containing the side chain found in the GLP-1 peptide tirzepatide, with TFA-free cleavage. pNZ was successfully utilized as the side chain PG showing full conversion under mild fully aqueous conditions. The target peptide was successfully synthesized with a purity of 40%, demonstrating the feasibility of fully aqueous and PFAS-free synthesis of ε-Lys branched peptides in ASPPS.}},
  author       = {{Nilsson, Olov}},
  language     = {{eng}},
  note         = {{Student Paper}},
  title        = {{Aqueous solid-phase peptide synthesis (ASPPS) approaches to complex peptides}},
  year         = {{2026}},
}