@misc{9240516,
  abstract     = {{High grade serous ovarian cancer (HGSOC) is accompanied by an immune-rich but heterogenous tumor microenvironment. Although tumor associated tertiary lymphoid structures (TLS) are linked to favourable antitumor immunity, B cells may also adopt spatial distribution patterns favourable for immune suppression. This study evaluated whether the abundance and spatial distribution patterns of CD20+ B cells were associated with overall survival in HGSOC. Eight tissue microarray (TMA) slides were analyzed by chromogenic immunohistochemistry (IHC) for CD20 and CD3. Cells were segmented in QuPath using a optimised Cellpose-SAM model and further classified based on their marker intensities. Binary Moran’s Index quantified the CD20+ cell autocorrelation, while a random labelling adjusted Ripley cross-L metric summarised CD20-CD3 spatial interactions. Image derived variables were collected and aggregated on a patient basis and evaluated using Cox proportional hazard regression models. CD20 abundance was not associated with overall survival after clinical adjustment. In contrast, higher CD20+ cell clustering was associated with poorer overall survival. Each 0.1-unit increase in patient-level mean binary Moran's I corresponded to an adjusted hazard ratio of 1.26 (95% CI, 1.00-1.57; p = 0.046). Interaction of CD20-CD3 cells was not independently prognostic. These findings indicate that the spatial distribution of B cells might be more informative than their abundance alone and support future phenotypic analysis of regulatory B cells in spatial studies of ovarian cancer.}},
  author       = {{Choudhary, Arnab}},
  language     = {{eng}},
  note         = {{Student Paper}},
  title        = {{Prognostic Impact of Spatial Distribution of B-cells in High-grade Serous Ovarian Cancer (HGSOC)}},
  year         = {{2026}},
}