@misc{9244518,
  abstract     = {{Ovarian cancer is one of the most lethal gynecological-related cancers and has a
high recurrence rate. Its heterogeneity and immunosuppressive landscape pose challenges when assessing treatment options. Novel immunotherapy strategies have potential to overcome its challenges with heterogeneity and immunosuppression but have not been successful yet. Utilizing the cGAS-STING pathway is promising for
immunosuppressive cancers, particularly using STING signaling as a method to prime the immune system. However, suitable models are required to evaluate STING signaling for ovarian cancer, especially considering its multiple histotypes. In this study, two histotypes were selected: low grade serous ovarian cancer (LGSOC) and high grade serous ovarian cancer (HGSOC). Four cell lines were assessed as potential models for STING signaling: HEY and ES2 representing low grade serous ovarian cancer-like models and OVCAR3 (BRCA wildtype) and KURAMOCHI (BRCA2 mutation) representing high grade serous ovarian cancer-like models. Western Blots demonstrate that STING expression is only present in HEY and KURAMOCHI. Furthermore, downstream targets TBK-1 is minimally expressed in all cell lines while IRF-3 shows clear bands. Though inconclusive, these results suggest that the variation in STING expression between cell lines may be attributed to baseline STING expression rather than activation of the cGAS-STING pathway. Variations in expression between the BRCA2 mutated vs wildtype high grade serous ovarian cancer-like model suggest a need for further subtype categorization. The low grade serous and high grade serous ovarian cancer models demonstrate heterogeneity within them and therefore cell line models should be validated based on baseline STING signaling instead of the histotype they reflect.}},
  author       = {{Sampermans, Isis Elena Maria}},
  language     = {{eng}},
  note         = {{Student Paper}},
  title        = {{Investigating STING-directed therapy for ovarian cancer using in-vitro models.}},
  year         = {{2026}},
}