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In vivo Quantification of Absolute Liver Metabolite Concentratins by 31P NMR Spectroscopy

Ressner, Marcus (2000)
Medical Physics Programme
Abstract (Swedish)
Phosphorus magnetic resonance spectroscopy (31P NMR) is a non-invasive technique for determining concentrations of intracellular metabolites linked to energy metabolism of human tissues. Inorganic phosphate (Pi) and adenosine triphosphate (ATP) are found in most tis-sues, while tissue specific metabolites such as creatine phosphate (PCr) and metabolites linked to lipid metabolism can be observed in organs such as the liver. During recent years, 31P NMR spectroscopy has been found to be valuable for studies of the biochemical composition of human tissues, and it is therefore of considerable value for diagnostics and prognosis in a wide variety of diseases. The purpose of this study was to investigate and set-up an in vivo quantification... (More)
Phosphorus magnetic resonance spectroscopy (31P NMR) is a non-invasive technique for determining concentrations of intracellular metabolites linked to energy metabolism of human tissues. Inorganic phosphate (Pi) and adenosine triphosphate (ATP) are found in most tis-sues, while tissue specific metabolites such as creatine phosphate (PCr) and metabolites linked to lipid metabolism can be observed in organs such as the liver. During recent years, 31P NMR spectroscopy has been found to be valuable for studies of the biochemical composition of human tissues, and it is therefore of considerable value for diagnostics and prognosis in a wide variety of diseases. The purpose of this study was to investigate and set-up an in vivo quantification method for 31P NMR of human liver metabolite concentrations; a method that would be easy to operate and to use routinely in a clinical environment. Furthermore, the overall aim of the project was to characterise the metabolic conditions in patients with liver cirrhosis. This included quantification of metabolite concentra-tions, improving the signal processing, and designing a prototype protocol for clinical measurements. The relatively low sensitivity of phosphorous NMR spectroscopy, together with the fact that the phosphorus concentration in the tissue examined often is in the mM range implied relatively long acquisition times in order to obtain sufficient signal-to-noise ratio. The method used in this study incorporated volume calculations from surface coil images and phantom measurements, as well as the development of a coil specific sensitivity map to correct for variations of the selected volume due to B11 inhomogeneity. Spectral analysis were performed exclusively in the time domain using MRUI and the VARPRO non-linear least square algorithm. The absolute concentrations of liver metabolites for healthy volunteers found in this study were for phosphomonoesters (PME) = 1.7, Pi = 2.0, phosphodi-esters (PDE) = 5.5, and ATP = 3.6 mM. (Less)
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author
Ressner, Marcus
supervisor
organization
year
type
H2 - Master's Degree (Two Years)
subject
keywords
MRI
language
English
id
2156893
date added to LUP
2011-09-13 14:51:34
date last changed
2011-09-13 14:51:34
@misc{2156893,
  abstract     = {Phosphorus magnetic resonance spectroscopy (31P NMR) is a non-invasive technique for determining concentrations of intracellular metabolites linked to energy metabolism of human tissues. Inorganic phosphate (Pi) and adenosine triphosphate (ATP) are found in most tis-sues, while tissue specific metabolites such as creatine phosphate (PCr) and metabolites linked to lipid metabolism can be observed in organs such as the liver. During recent years, 31P NMR spectroscopy has been found to be valuable for studies of the biochemical composition of human tissues, and it is therefore of considerable value for diagnostics and prognosis in a wide variety of diseases. The purpose of this study was to investigate and set-up an in vivo quantification method for 31P NMR of human liver metabolite concentrations; a method that would be easy to operate and to use routinely in a clinical environment. Furthermore, the overall aim of the project was to characterise the metabolic conditions in patients with liver cirrhosis. This included quantification of metabolite concentra-tions, improving the signal processing, and designing a prototype protocol for clinical measurements. The relatively low sensitivity of phosphorous NMR spectroscopy, together with the fact that the phosphorus concentration in the tissue examined often is in the mM range implied relatively long acquisition times in order to obtain sufficient signal-to-noise ratio. The method used in this study incorporated volume calculations from surface coil images and phantom measurements, as well as the development of a coil specific sensitivity map to correct for variations of the selected volume due to B11 inhomogeneity. Spectral analysis were performed exclusively in the time domain using MRUI and the VARPRO non-linear least square algorithm. The absolute concentrations of liver metabolites for healthy volunteers found in this study were for phosphomonoesters (PME) = 1.7, Pi = 2.0, phosphodi-esters (PDE) = 5.5, and ATP = 3.6 mM.},
  author       = {Ressner, Marcus},
  keyword      = {MRI},
  language     = {eng},
  note         = {Student Paper},
  title        = {In vivo Quantification of Absolute Liver Metabolite Concentratins by 31P NMR Spectroscopy},
  year         = {2000},
}