Advanced

WIDE-BANDWIDTH TIME OF FLIGHT SPECTROSCOPY OF TURBID MEDIA

Subash, Arman Ahamed LU (2012) In Lund Reports in Atomic Physics PHYM01 20112
Atomic Physics
Abstract
Wide bandwidth time-of-flight spectrometer (TOFS) developed in the Group of Biophotonics,Lund University, is a unique tool which is capable to deliver continues absorption/scattering spectra of turbid samples in a singularly broad wavelength range from 600nm up to 1400nm. The main focus of this thesis work is to calibrate and optimize the performance of the instrumental setup of this system for biomedical & pharmaceutical applications ensuring the capability to produce results with high accuracy and repeatability. In this spectroscopy, the precision of the measurements heavily relies on precise timing calibration of the system and accurate determination of the instrumental response function (IRF). A new double path optical scheme was... (More)
Wide bandwidth time-of-flight spectrometer (TOFS) developed in the Group of Biophotonics,Lund University, is a unique tool which is capable to deliver continues absorption/scattering spectra of turbid samples in a singularly broad wavelength range from 600nm up to 1400nm. The main focus of this thesis work is to calibrate and optimize the performance of the instrumental setup of this system for biomedical & pharmaceutical applications ensuring the capability to produce results with high accuracy and repeatability. In this spectroscopy, the precision of the measurements heavily relies on precise timing calibration of the system and accurate determination of the instrumental response function (IRF). A new double path optical scheme was implemented in this system, for adding Time Reference Pulse to circumvent the uncertainty occurred in the results due to apparent source and detector temporal drifts and finite resolution of the TCSPC detection. This technique brings success to maintain high measurement precision (uncertainty less than 1 %). The accurate calibration of the system was performed by measuring the reduced scattering coefficient (μ’s) of pure Intralipid-20% and absorption coefficient (μa) of Indian ink solution. For performance assessment of the system, different types of experiments including verification of repeatability, verification of linearity in performance, verification of the consistency of the experimental data along with theoretical model were executed preparing different type of solid and liquid phantoms. All these experiments showed acceptable results. New type of epoxy phantom was developed with BG36 filter crashed powder and TiO2 for providing remarkably different absorptions at different wavelengths which is very effective for calibration of the system. Limited resolution effects of the system for finite source resolution and dispersion in probe pulse were also explored using this phantom. Time-of-flight spectroscopy (TOFS) was also implemented in order to measure Active Pharmaceutical Ingredient (API) of the pharmaceutical tablets. Continuous scattering and absorption spectra of real tablets were acquired at NIR region with the resolution/step of 4 nm. The estimated API concentrations by PLS regression are within 10% of the reference values. (Less)
Please use this url to cite or link to this publication:
author
Subash, Arman Ahamed LU
supervisor
organization
alternative title
WIDE-BANDWIDTH TIME OF FLIGHT SPECTROSCOPY OF TURBID MEDIA
course
PHYM01 20112
year
type
H2 - Master's Degree (Two Years)
subject
keywords
WIDE-BANDWIDTH, TIME OF FLIGHT, SPECTROSCOPY, TURBID
publication/series
Lund Reports in Atomic Physics
report number
LRAP-458
language
English
id
3053369
date added to LUP
2012-11-28 22:35:11
date last changed
2012-11-28 22:35:11
@misc{3053369,
  abstract     = {Wide bandwidth time-of-flight spectrometer (TOFS) developed in the Group of Biophotonics,Lund University, is a unique tool which is capable to deliver continues absorption/scattering spectra of turbid samples in a singularly broad wavelength range from 600nm up to 1400nm. The main focus of this thesis work is to calibrate and optimize the performance of the instrumental setup of this system for biomedical & pharmaceutical applications ensuring the capability to produce results with high accuracy and repeatability. In this spectroscopy, the precision of the measurements heavily relies on precise timing calibration of the system and accurate determination of the instrumental response function (IRF). A new double path optical scheme was implemented in this system, for adding Time Reference Pulse to circumvent the uncertainty occurred in the results due to apparent source and detector temporal drifts and finite resolution of the TCSPC detection. This technique brings success to maintain high measurement precision (uncertainty less than 1 %). The accurate calibration of the system was performed by measuring the reduced scattering coefficient (μ’s) of pure Intralipid-20% and absorption coefficient (μa) of Indian ink solution. For performance assessment of the system, different types of experiments including verification of repeatability, verification of linearity in performance, verification of the consistency of the experimental data along with theoretical model were executed preparing different type of solid and liquid phantoms. All these experiments showed acceptable results. New type of epoxy phantom was developed with BG36 filter crashed powder and TiO2 for providing remarkably different absorptions at different wavelengths which is very effective for calibration of the system. Limited resolution effects of the system for finite source resolution and dispersion in probe pulse were also explored using this phantom. Time-of-flight spectroscopy (TOFS) was also implemented in order to measure Active Pharmaceutical Ingredient (API) of the pharmaceutical tablets. Continuous scattering and absorption spectra of real tablets were acquired at NIR region with the resolution/step of 4 nm. The estimated API concentrations by PLS regression are within 10% of the reference values.},
  author       = {Subash, Arman Ahamed},
  keyword      = {WIDE-BANDWIDTH,TIME OF FLIGHT,SPECTROSCOPY,TURBID},
  language     = {eng},
  note         = {Student Paper},
  series       = {Lund Reports in Atomic Physics},
  title        = {WIDE-BANDWIDTH TIME OF FLIGHT SPECTROSCOPY OF TURBID MEDIA},
  year         = {2012},
}