Investigating the role of miR-152 and miR-130a in Glucose-Stimulated Insulin Secretion
(2013) MOBN19 20122Degree Projects in Molecular Biology
- Abstract
- Investigating the role of miRNAs in Glucose-Stimulated Insulin Secretion
MicroRNAs have emerged as important players in many aspects of fundamental biological processes including regulation of pancreatic β-cell functions. Failure in beta cell functions, specifically with regards to glucose-stimulated insulin secretion (GSIS), contributes in the pathogenesis of type-2 diabetes (T2D). Previous studies in a T2D rodent model, Goto-Kakizaki (GK) rat, implicated dysregulated microRNAs as contributing factors in impaired GSIS.
Here, we investigated two such miRNAs in two clonal beta cell lines which display diametrically opposite phenotypes: INS1-832/13 which readily responds to glucose stimulation and secretes high amount of insulin and... (More) - Investigating the role of miRNAs in Glucose-Stimulated Insulin Secretion
MicroRNAs have emerged as important players in many aspects of fundamental biological processes including regulation of pancreatic β-cell functions. Failure in beta cell functions, specifically with regards to glucose-stimulated insulin secretion (GSIS), contributes in the pathogenesis of type-2 diabetes (T2D). Previous studies in a T2D rodent model, Goto-Kakizaki (GK) rat, implicated dysregulated microRNAs as contributing factors in impaired GSIS.
Here, we investigated two such miRNAs in two clonal beta cell lines which display diametrically opposite phenotypes: INS1-832/13 which readily responds to glucose stimulation and secretes high amount of insulin and INS1-832/2, which exhibit very low insulin secretion upon glucose stimulation.
Quantification of miRNAs using qRT-PCR shows that both miRNAs are up-regulated in INS1-832/2 cells reflecting the findings in the pancreatic islets of T2D model GK rat. Overexpression of both miRNAs in INS1-832/13 cells reduces insulin secretion by down-regulating important metabolic enzymes necessary for β-cell function during GSIS.
We conclude that these miRNAs play important roles in cellular functions necessary for insulin secretion.
Advisor: Jonathan Esguerra
Master´s Degree Project 45 credits in Molecular Biology, Molecular Genetics 2013
Department of Biology, Lund University (Less)
Please use this url to cite or link to this publication:
http://lup.lub.lu.se/student-papers/record/3631998
- author
- Ofori, Jones
- supervisor
- organization
- course
- MOBN19 20122
- year
- 2013
- type
- H2 - Master's Degree (Two Years)
- subject
- language
- English
- id
- 3631998
- date added to LUP
- 2013-04-09 12:29:03
- date last changed
- 2013-04-10 09:26:55
@misc{3631998,
abstract = {{Investigating the role of miRNAs in Glucose-Stimulated Insulin Secretion
MicroRNAs have emerged as important players in many aspects of fundamental biological processes including regulation of pancreatic β-cell functions. Failure in beta cell functions, specifically with regards to glucose-stimulated insulin secretion (GSIS), contributes in the pathogenesis of type-2 diabetes (T2D). Previous studies in a T2D rodent model, Goto-Kakizaki (GK) rat, implicated dysregulated microRNAs as contributing factors in impaired GSIS.
Here, we investigated two such miRNAs in two clonal beta cell lines which display diametrically opposite phenotypes: INS1-832/13 which readily responds to glucose stimulation and secretes high amount of insulin and INS1-832/2, which exhibit very low insulin secretion upon glucose stimulation.
Quantification of miRNAs using qRT-PCR shows that both miRNAs are up-regulated in INS1-832/2 cells reflecting the findings in the pancreatic islets of T2D model GK rat. Overexpression of both miRNAs in INS1-832/13 cells reduces insulin secretion by down-regulating important metabolic enzymes necessary for β-cell function during GSIS.
We conclude that these miRNAs play important roles in cellular functions necessary for insulin secretion.
Advisor: Jonathan Esguerra
Master´s Degree Project 45 credits in Molecular Biology, Molecular Genetics 2013
Department of Biology, Lund University}},
author = {{Ofori, Jones}},
language = {{eng}},
note = {{Student Paper}},
title = {{Investigating the role of miR-152 and miR-130a in Glucose-Stimulated Insulin Secretion}},
year = {{2013}},
}