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Genomics of the wine yeast Dekkera bruxellensis

Ling, Zhihao (2012) BINP31 20112
Degree Projects in Bioinformatics
Abstract
Abstract

The yeast Dekkera/Brettanomyces bruxellensis is a distant relative of baker’s yeast Saccharomyces cerevisiae and either has positive or negative effects to beverage flavor development. D. bruxellensis and S. cerevisiae display several similar traits which play very important roles in food industry, such as production of high ethanol levels. In our lab, we determined and annotated the 13.4 Mb genome sequence of the D. bruxellensis strain Y879 (CBS2499) and investigated the genetic background of several “food-relevant” properties of this yeast as well we studied its evolution history and metabolic pathways, such as carbon metabolism. Surprisingly, we found out that D. bruxellensis is closely related to Pichia (Komagataella)... (More)
Abstract

The yeast Dekkera/Brettanomyces bruxellensis is a distant relative of baker’s yeast Saccharomyces cerevisiae and either has positive or negative effects to beverage flavor development. D. bruxellensis and S. cerevisiae display several similar traits which play very important roles in food industry, such as production of high ethanol levels. In our lab, we determined and annotated the 13.4 Mb genome sequence of the D. bruxellensis strain Y879 (CBS2499) and investigated the genetic background of several “food-relevant” properties of this yeast as well we studied its evolution history and metabolic pathways, such as carbon metabolism. Surprisingly, we found out that D. bruxellensis is closely related to Pichia (Komagataella) pastoris. Interestingly, we only found a low copy number of low affinity sugar transporters in D. bruxellensis. We also studied the main pathway of glucose repression, where Mig1p and Hxk2p play important roles. Just like in S. cerevisiae, the promoters of TCA genes in D. bruxellensis also had many Mig1p binding motifs. Further analysis on aroma related genes showed that the presence of aroma gene duplicates vary among species. However, some of them are duplicated in the species known to have intensive aromatic profile. Finally, the genome sequence of D. bruxellensis was used to build a database employing a special version of Yeast Genome Annotation Pipline (YGAP), a synteny-based pipeline for the automated annotation of yeast genomes. (Less)
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author
Ling, Zhihao
supervisor
organization
course
BINP31 20112
year
type
H2 - Master's Degree (Two Years)
subject
language
English
id
3632990
date added to LUP
2013-04-11 16:26:44
date last changed
2013-04-11 16:26:44
@misc{3632990,
  abstract     = {Abstract

The yeast Dekkera/Brettanomyces bruxellensis is a distant relative of baker’s yeast Saccharomyces cerevisiae and either has positive or negative effects to beverage flavor development. D. bruxellensis and S. cerevisiae display several similar traits which play very important roles in food industry, such as production of high ethanol levels. In our lab, we determined and annotated the 13.4 Mb genome sequence of the D. bruxellensis strain Y879 (CBS2499) and investigated the genetic background of several “food-relevant” properties of this yeast as well we studied its evolution history and metabolic pathways, such as carbon metabolism. Surprisingly, we found out that D. bruxellensis is closely related to Pichia (Komagataella) pastoris. Interestingly, we only found a low copy number of low affinity sugar transporters in D. bruxellensis. We also studied the main pathway of glucose repression, where Mig1p and Hxk2p play important roles. Just like in S. cerevisiae, the promoters of TCA genes in D. bruxellensis also had many Mig1p binding motifs. Further analysis on aroma related genes showed that the presence of aroma gene duplicates vary among species. However, some of them are duplicated in the species known to have intensive aromatic profile. Finally, the genome sequence of D. bruxellensis was used to build a database employing a special version of Yeast Genome Annotation Pipline (YGAP), a synteny-based pipeline for the automated annotation of yeast genomes.},
  author       = {Ling, Zhihao},
  language     = {eng},
  note         = {Student Paper},
  title        = {Genomics of the wine yeast Dekkera bruxellensis},
  year         = {2012},
}